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Use of mir-27b compound as marker of chronic pain and use in preparation of medicine for treating chronic pain

A mir-27b, compound technology, applied in the field of biomedical materials, can solve problems such as the use of which has not yet been reported, and achieve the effects of low cost, lower thermal shock threshold, and simple process

Inactive Publication Date: 2016-04-13
XUZHOU MEDICAL COLLEGE
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The use of miR-27b as a gene target to inhibit chronic pain and as a marker for chronic pain diagnosis has not been reported so far

Method used

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  • Use of mir-27b compound as marker of chronic pain and use in preparation of medicine for treating chronic pain
  • Use of mir-27b compound as marker of chronic pain and use in preparation of medicine for treating chronic pain
  • Use of mir-27b compound as marker of chronic pain and use in preparation of medicine for treating chronic pain

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Embodiment Construction

[0021] 1. Preparation of CFA chronic arthritis pain model and expression of miR-27b at spinal cord level:

[0022] SPF-grade male Kunming male mice of 6 weeks were anesthetized with isoflurane, and injected 40 μl of sterile complete Freund's adjuvant (CFA; Sigma Company) intradermally at two points around the metatarsal joint of the left hind foot. Injection concentrations can induce arthritis. The decrease in thermal and mechanical pain thresholds appeared within 24 hours after injection and was maintained for more than 3 weeks.

[0023] Expression analysis of MiR-27b: Fluorescent quantitative analysis of miR-27b was performed using the miRNA detection kit. figure 1 ).

[0024] 2. Using miR-27b mimics to overexpress spinal cord miRNAs in vivo.

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Abstract

The invention relates to application of a miR-27b compound used as a chronic pain marker and application of the miR-27b compound in preparing a medicament for treating chronic pain. The application comprises the following steps: (1) screening miR-27b target genes, verifying by use of a reporter gene, and making an inflammatory chronic pain model mouse by utilizing a CFA (Complete Freund Adjuvant); (2) increasing the expression quantity of miRNA in the spinal cord of the inflammatory chronic pain model mouse by utilizing a miR-27b simulant; (3) detecting the inhibiting action of miR-27b on the DOT1L protein expression of the CFA causing inflammatory chronic pain by utilizing a Western blot method; (4) detecting a pain behavior by utilizing a heat activation method. The process is simple and the cost is low; the inflammatory chronic pain is effectively inhibited by adopting the miR-27b and an effective intervention medicament for preventing and treating the chronic pain is provided. The invention particularly relates to the application of the miR-27b compound used as the chronic pain marker and the application of the miR-27b compound in preparing the medicament for treating the chronic pain. The application disclosed by the invetnion indicates that the miR-27b can be taken as the marker of chronic pain generation by further carrying out quantitative analysis on the clinical blood specimen of a chronic pain patient.

Description

technical field [0001] The present invention relates to the use of a microRNA molecule, in particular to the use of the miR-27b compound, specifically the use of the miR-27b compound as a marker of chronic pain and its use in the preparation of medicines for treating chronic pain. It belongs to the technical field of biomedical materials. Background technique [0002] Pain is one of the most common clinical symptoms. Long-term pathological pain accompanied by psychological and emotional diseases has brought unbearable torture to the lives of chronic pain patients. According to statistics, there are as many as 320 million chronic pain patients in the world. Governments around the world have invested a lot of manpower and hundreds of billions of dollars in financial resources to try to overcome this problem. In China alone, there are hundreds of millions of pain patients. Undoubtedly, it is a difficult problem that human beings can't wait to solve. [0003] Nerve cells conta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A61K48/00A61P29/00A61P25/04
CPCA61K48/0066C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 郭羽白尹翠韩文灿
Owner XUZHOU MEDICAL COLLEGE
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