Preparation method of osteoarthritis animal model

Abstract

The invention relates to a preparation method of an osteoarthritis animal model. The method comprises the following steps: blending a matrix metalloproteinase-3 with normal saline so as to obtain a 5-10nmol / L matrix metalloproteinase-3 solution; blending a urokinase-type plasminogen activator with the normal saline so as to obtain a 2-4nmol / L urokinase-type plasminogen activator solution; then, blending the two solutions in proportion of 3:1 or 2:1 so as to obtain a mixed solution; injecting 0.5mL / kg of mixed solution into an articular cavity of an experimental animal. According to the method, the matrix metalloproteinase-3 and the urokinase-type plasminogen activator which are of conventional chemical reagents in a laboratory are taken as raw materials; the mixture is injected into the articular cavity of the animal, so that the rapid degeneration of cartilage tissues is generated. Thus, the pathogenesis process of osteoarthritis is simulated, thereby bringing great convenience for the research of an osteoarthritis intervention treatment technology. The method has the characteristics of being simple and convenient to operate, rapid in modelling, economical, practical, convenient in material obtainment, and the like.

Description

technical field [0001] The invention relates to a method for preparing an animal model of osteoarthritis. Background technique [0002] Osteoarthritis has become a major bone and joint disease that seriously affects middle-aged and elderly patients around the world. Due to its complex etiology and no clear pathogenesis, it has brought great difficulties to effective clinical treatment. It is now known that matrix metalloproteinases are joint factors of cartilage degeneration in osteoarthritis, and existing studies have shown that the application of matrix metalloproteinase inhibitors can significantly alleviate the process of cartilage degeneration in osteoarthritis. However, in the process of cartilage degeneration in osteoarthritis, the matrix metalloproteinases secreted by chondrocytes and synoviocytes all exist in the form of zymogens, which are mainly activated by urokinase-type plasminogen activator before becoming active enzymes. Cartilage degeneration. [0003] Res...

AI Technical Summary

Problems solved by technology

[0003] The research on the mechanism of osteoarthritis and intervention measures must be based on animal models. The existing animal models of osteoarthritis are mainly based on joint trauma caused by surgical cutting of the fork ligament, which cannot truly reflect the entire pathological mechanism of osteoarthritis.

However, the arthritis models caused by chemical methods such as collagenase and papain often have significant immune responses, and cannot truly reflect the pathological mechanism of osteoarthritis.