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A kind of hangchrysanthemum polysaccharide and its preparation method and application

A technology of chrysanthemum and polysaccharides, applied in the direction of drug combination, antineoplastic drugs, etc., can solve the problems of low effective rate and damage

Active Publication Date: 2017-02-08
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The short-term effective rate of most drugs is lower than 10%, and it is accompanied by side effects that bring great pain to the patient's body and mind, especially the damage to liver and kidney function (Nitsche, M., et al. Chemoradioimmunotherapy with 5-fluorouracil, cisplatin and interferon-alphain Pancreatic and periampullary cancer: results of a feasibility study. Cancer Radiotherapy, 2008, 12(8), 817-821.)

Method used

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  • A kind of hangchrysanthemum polysaccharide and its preparation method and application
  • A kind of hangchrysanthemum polysaccharide and its preparation method and application
  • A kind of hangchrysanthemum polysaccharide and its preparation method and application

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Experimental program
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Effect test

experiment Embodiment 1

[0030] a. Polysaccharide extraction:

[0031] 3.8kg dried Chrysanthemum chrysanthemum (origin: Tongxiang, Zhejiang, purchased from Shanghai Donghu Qingyutang Guoyao Co., Ltd.), added 50L95% ethanol to degrease twice, each time for 3 days, after degreasing, the chrysanthemum was dried naturally at room temperature, and degreased The last chrysanthemum flower 3.7kg, extracted with boiling water, adding 80L of water each time, 4h each time, the sugar content of the extract was detected by the sulfuric acid-phenol method, until the sugar reaction was not obvious, a total of 6 extractions were made, and the extracts were combined and filtered , the extract was heated and concentrated to about 10L under normal pressure, dialyzed against flowing water for 2 days, the liquid in the dialysis bag was concentrated to about 5L, the concentrated solution was centrifuged, the supernatant was added to 4 times the volume of 95% ethanol under vigorous stirring, and the temperature was 4°C. Let...

experiment Embodiment 2

[0038] MTT (tetrazolium salt method) experiment

[0039] In the experiment, human pancreatic cancer cell line PANC-1 and human normal liver cell line LO2 (Cell Bank of Type Culture Collection Committee of Chinese Academy of Sciences, Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences) were used to culture in 10% fetal bovine serum (Gibco Company) ), 100U / mL penicillin and 100U / mL streptomycin in DMEM medium (HyClone). Cells at 37°C with 5% CO 2 cultured in an incubator.

[0040] Inoculate 100 μL / well of LO2 cells in the logarithmic growth phase in a 96-well microculture plate with a cell concentration of 8×10 5 individual / mL. 37°C, 5% CO 2 Incubate in the incubator for 24h. Add 100 μL of medium solution containing neutral and uniform polysaccharide from Chrysanthemum chrysanthemum CMTA0S3, so that the final concentration is 4.1, 12.3, 37.0, 111.1, 333.3 and 1000.0 μg / mL, each concentration is three duplicate wells, and a blank c...

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Abstract

The invention discloses a chrysanthemum morifolium cv.Hangju polysaccharide extraction method, chrysanthemum morifolium cv.Hangju polysaccharide prepared by the extraction method, and use of the chrysanthemum morifolium cv.Hangju polysaccharide in preparation of anti-tumor medicines.

Description

technical field [0001] The present invention relates to polysaccharides, their extraction methods and their use in the preparation of medicines, in particular to a method for extracting polysaccharides from Chrysanthemum morifolium cv. Hangju, the polysaccharides extracted by the method and the polysaccharides Use in the preparation of antitumor drugs. Background technique [0002] Pancreatic cancer is one of the common malignant tumors of the digestive tract, which mostly occurs in the head of the pancreas (Jemal, A., et al. 2010. Cancer statistics. A Cancer Journal for Clinicians, 2010, 60, 277-300.). Pancreatic cancer is known as the king of cancers because of its low surgical success rate, rapid disease progression, and severe pain for patients. Most of them focus on short-term treatment to relieve symptoms and prolong life. The average time from the discovery of the tumor to the death of patients is only 3-6 months. [0003] It is clinically found that pancreatic can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 丁侃范灵婧
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI