Method for rapidly identifying black shank resistance at tobacco seedling stage

A technology for black shank and tobacco, applied in the field of plant disease resistance identification, can solve the problems of limited number of identified varieties, large workload, long time, etc., and achieve the effect of improving the standardization degree of tests, small differences, and improving test speed.

Inactive Publication Date: 2014-11-19
GUIZHOU TOBACCO SCI RES INST
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Problems solved by technology

Due to the influence of soil fertility and seasons, the number of varieties identified each year is greatly limited by the field blackleg identification method, and there are certain differences in the resistance of varieties identified between years; and the existing identification methods at the seedling stage require transplanting after seedling cultivation. To flowerpots, indoor disease gardens or transplanted to new trays, the disadvantage is that it takes a long time and a lot of work to inoculate after transplanting the seedlings

Method used

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  • Method for rapidly identifying black shank resistance at tobacco seedling stage
  • Method for rapidly identifying black shank resistance at tobacco seedling stage

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Experimental program
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Effect test

Embodiment 1

[0017] 1. Materials and methods

[0018] 1.1. Test materials

[0019] Tobacco varieties / lines in Guizhou Province, 10 copies of flue-cured tobacco materials, compared with innovation 3, Jinxing 6007, and Xiaojin 1025.

[0020] 1.2. Test method

[0021] 1.2.1. Conventional disease nursery identification method

[0022] Field disease identification was carried out in the black shank identification nursery of flue-cured tobacco variety experiment of Guizhou Tobacco Research Institute. There were 4 repetitions in the experiment, and 10 tobacco plants were planted in each row. The floating seedling raising method is adopted, and the seedling raising method is the same as conventional production. Cut the leaves twice, cultivate strong seedlings, and transplant them in early May, with a density of 1×0.6m 2 , 6 kg of pure nitrogen per mu. Other cultivation management measures are the same as field production. Let Gexin No. 3 be the disease-resistant control, Jinxing 6007 be the...

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Abstract

The invention discloses a method for rapidly identifying the black shank resistance at a tobacco seedling stage, which comprises the following steps: seeding tobacco to be identified into a seeding hole tray to raise seedlings, carrying out artificial inoculation after the age of each seedling is 35-45 days, embedding 0.1-1 g of pre-cultivated black shank inoculation materials in a hole of the hole tray for all selected tobacco plants, respectively investigating disease classification within 3-10 days after inoculation and calculating disease indexes. The method has the advantages that an identification method that the seedlings are not transplanted is adopted, no wound is artificially created, and good rapidity and convenience are realized; through many years of conventional field disease nursery identification and the synchronous verification of the method, the identification results obtained through the method are equivalent to the field identification results, the annual difference is small, the identification time is short, and the workload is small.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, in particular to a method for identifying tobacco black shank resistance. Background technique [0002] Tobacco black shank is one of the most devastating diseases in tobacco production, and it is also one of the most serious diseases in the world's tobacco production. The disease occurs to varying degrees in various tobacco-producing provinces in my country, and the damage is relatively common and seriously. Selecting tobacco varieties resistant to black shank is an important way to solve this disease. [0003] At present, the existing identification methods for disease resistance of tobacco varieties mainly include field identification method of blackleg disease nursery and seedling identification method. Due to the soil force and season, the number of varieties identified each year is greatly limited by the field blackleg identification method, and the varietie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12N1/14A01G7/00
Inventor 王仁刚林世锋王轶曾吉凡
Owner GUIZHOU TOBACCO SCI RES INST
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