A method for rapid identification of black shank resistance in tobacco seedling stage
A black shank and tobacco technology, applied in the field of plant disease resistance identification, can solve the problems of limited number of identification varieties, heavy workload, long time, etc., and achieve the effect of improving test standardization, small differences, and increasing test speed
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[0017] 1. Materials and methods
[0018] 1.1. Test materials
[0019] Tobacco varieties / lines in Guizhou Province, 10 copies of flue-cured tobacco materials, compared with innovation 3, Jinxing 6007, and Xiaojin 1025.
[0020] 1.2. Test method
[0021] 1.2.1. Conventional disease nursery identification method
[0022] Field disease identification was carried out in the black shank identification nursery of flue-cured tobacco variety experiment of Guizhou Tobacco Research Institute. There were 4 repetitions in the experiment, and 10 tobacco plants were planted in each row. The floating seedling raising method is adopted, and the seedling raising method is the same as conventional production. Cut the leaves twice, cultivate strong seedlings, and transplant them in early May, with a density of 1×0.6m 2 , 6 kg of pure nitrogen per mu. Other cultivation management measures are the same as field production. Let Gexin No. 3 be the disease-resistant control, Jinxing 6007 be the...
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