A method for rapid identification of black shank resistance in tobacco seedling stage

A black shank and tobacco technology, applied in the field of plant disease resistance identification, can solve the problems of limited number of identification varieties, heavy workload, long time, etc., and achieve the effect of improving test standardization, small differences, and increasing test speed

Inactive Publication Date: 2016-06-29
GUIZHOU TOBACCO SCI RES INST
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  • Application Information

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Problems solved by technology

Due to the influence of soil fertility and seasons, the number of varieties identified each year is greatly limited by the field blackleg identification method, and there are certain differences in the resistance of varieties identified between years; and the existing identification methods at the seedling stage require transplanting after seedling cultivation. To flowerpots, indoor disease gardens or transplanted to new trays, the disadvantage is that it takes a long time and a lot of work to inoculate after transplanting the seedlings

Method used

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  • A method for rapid identification of black shank resistance in tobacco seedling stage
  • A method for rapid identification of black shank resistance in tobacco seedling stage

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Experimental program
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Effect test

Embodiment 1

[0017] 1. Materials and methods

[0018] 1.1. Test materials

[0019] Tobacco varieties / lines in Guizhou Province, 10 copies of flue-cured tobacco materials, compared with innovation 3, Jinxing 6007, and Xiaojin 1025.

[0020] 1.2. Test method

[0021] 1.2.1. Conventional disease nursery identification method

[0022] Field disease identification was carried out in the black shank identification nursery of flue-cured tobacco variety experiment of Guizhou Tobacco Research Institute. There were 4 repetitions in the experiment, and 10 tobacco plants were planted in each row. The floating seedling raising method is adopted, and the seedling raising method is the same as conventional production. Cut the leaves twice, cultivate strong seedlings, and transplant them in early May, with a density of 1×0.6m 2 , 6 kg of pure nitrogen per mu. Other cultivation management measures are the same as field production. Let Gexin No. 3 be the disease-resistant control, Jinxing 6007 be the...

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Abstract

The invention discloses a method for quickly identifying the resistance to black shank disease at the young seedling stage of tobacco. The tobacco varieties to be identified are sown into seedling trays to raise seedlings. When the seedlings are 35 to 45 days old, artificial inoculation is performed. Tobacco plants were embedded with 0.1-1 g of pre-cultivated black shank inoculation materials in the holes of the plug trays, and the disease grading was investigated 3 to 10 days after inoculation, and the disease index was calculated. The present invention adopts the identification method of seedlings without transplanting / transplanting, does not artificially create wounds, and is faster and more convenient. After many years of conventional field disease nursery identification and simultaneous verification of the method of the present invention, the identification results of the present invention are equivalent to field identification, and the annual The difference is small, the identification time is short, and the workload is small.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, in particular to a method for identifying tobacco black shank resistance. Background technique [0002] Tobacco black shank is one of the most devastating diseases in tobacco production, and it is also one of the most serious diseases in the world's tobacco production. The disease occurs to varying degrees in various tobacco-producing provinces in my country, and the damage is relatively common and seriously. Selecting tobacco varieties resistant to black shank is an important way to solve this disease. [0003] At present, the existing identification methods for disease resistance of tobacco varieties mainly include field identification method of blackleg disease nursery and seedling identification method. Due to the soil force and season, the number of varieties identified each year is greatly limited by the field blackleg identification method, and the varietie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18C12N1/14A01G7/00
Inventor 王仁刚林世锋王轶曾吉凡
Owner GUIZHOU TOBACCO SCI RES INST
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