Method for detecting carbendazol and thiabendazole residues in fruit and vegetable juice through liquid chromatogram
A liquid chromatography, fruit and vegetable juice technology, applied in the field of detection, can solve the problems of large dilution volume, environmental pollution, and high risk of detection personnel's work, and achieve the effect of simple steps and low toxicity
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Embodiment 1
[0027] Carbendazim and Thiabendazole were analyzed by liquid chromatography.
[0028] Pretreatment of non-spiked samples: mix fruit and vegetable juices evenly, accurately weigh 12.5g of samples into a 25mL colorimetric tube, dilute to 25mL with 0.2mol / L hydrochloric acid solution, vortex and mix for 1min, transfer to a 50mL polypropylene centrifuge centrifuge at 4000r / min for 2min at room temperature; activate the Bond Elut Plexa PCX solid-phase extraction column with 3mL of methanol and 3mL of first-grade water respectively, accurately pipette 10mL of the centrifuged supernatant to pass through the column, and then use 3mL of first-grade water Purify the solid-phase extraction column with 3 mL of methanol, and elute with 6 mL of 3% ammonia water and methanol; blow the eluent to dryness at room temperature with nitrogen, dissolve the residue with 0.1 mol / L hydrochloric acid and dilute to 1 mL, pass through a 0.45 μm filter membrane, and set aside.
[0029] Liquid ...
Embodiment 2
[0037] Carbendazim and Thiabendazole were analyzed by liquid chromatography.
[0038] Pretreatment of non-spiked samples: mix fruit and vegetable juices evenly, accurately weigh 12.5g of samples into a 25mL colorimetric tube, dilute to 25mL with 0.2mol / L hydrochloric acid solution, vortex and mix for 1min, transfer to a 50mL polypropylene centrifuge centrifuge at 4000r / min for 2min at room temperature; activate the Bond Elut Plexa PCX solid-phase extraction column with 3mL of methanol and 3mL of first-grade water respectively, accurately pipette 10mL of the centrifuged supernatant to pass through the column, and then use 3mL of first-grade water Purify the solid-phase extraction column with 3 mL of methanol, and elute with 6 mL of 3% ammonia water and methanol; blow the eluent to dryness at room temperature with nitrogen, dissolve the residue with 0.1 mol / L hydrochloric acid and dilute to 1 mL, pass through a 0.45 μm filter membrane, and set aside.
[0039] Liquid chro...
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