Rapid propagation method of polyploidy mutation of cissampelos pareira

A polyploid, rapid technology, applied in the field of plants, to achieve the effect of high utilization rate, large-scale planting and high survival rate

Inactive Publication Date: 2015-01-07
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medicinal effect is to reduce swelling, relieve pain, stop bleeding, and promote muscle growth. At present, there is no artificial propagation of tin vine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the pollen of vine vine, disinfect it with 70% ethanol for 0.5min, then sterilize it with 1% hydrogen peroxide solution for 5 minutes, wash it with sterile water 5 times, and inoculate the sterilized vine pollen into MS+NAA0. 4mg / L+KT6mg / L+colchicine 50mg / L medium for callus induction, add sucrose 30g / L, agar 6.5g / L, pH5.8, dark light, temperature 22±2℃, induction The dispersed and stable callus was inoculated into the medium of VR+TDZ2mg / L+NAA0.1mg / L+tryptophan 24μmol / L for callus differentiation, adding sucrose 30g / L, agar 6.5g / L, pH 5.8, light 2500lx, light period 12h / d, temperature 23±2°C, differentiated embryos were placed in MS medium for strong seedling growth, supplemented with sucrose 60g / L, agar 7g / L, pH 5.8, light 20000lx, photoperiod 14h / d, temperature 28℃, survival rate 80%.

Embodiment 2

[0011] Take the pollen of vine vine, disinfect it with 70% ethanol for 0.5min, then sterilize it with 1% hydrogen peroxide solution for 5 minutes, wash it with sterile water 5 times, and inoculate the sterilized vine pollen into MS+NAA0. 4mg / L+KT6mg / L+colchicine 100mg / L medium for callus induction, add sucrose 30g / L, agar 6.5g / L, pH5.8, dark light, temperature 22±2℃, induction The dispersed and stable callus was inoculated into the medium of VR+TDZ2mg / L+NAA0.1mg / L+tryptophan 24μmol / L for callus differentiation, adding sucrose 30g / L, agar 6.5g / L, pH 5.8, light 2500lx, light period 12h / d, temperature 23±2°C, differentiated embryos were placed in MS medium for strong seedling growth, supplemented with sucrose 60g / L, agar 7g / L, pH 5.8, light 20000lx, photoperiod 14h / d, temperature 28℃, survival rate 82%.

Embodiment 3

[0013] Take the pollen of vine vine, disinfect it with 70% ethanol for 0.5min, then sterilize it with 1% hydrogen peroxide solution for 5 minutes, wash it with sterile water 5 times, and inoculate the sterilized vine pollen into MS+NAA0. 4mg / L+KT6mg / L+colchicine 150mg / L medium for callus induction, add sucrose 30g / L, agar 6.5g / L, pH5.8, dark light, temperature 22±2℃, induction The dispersed and stable callus was inoculated into the medium of VR+TDZ2mg / L+NAA0.1mg / L+tryptophan 24μmol / L for callus differentiation, adding sucrose 30g / L, agar 6.5g / L, pH 5.8, light 2500lx, light period 12h / d, temperature 23±2°C, differentiated embryos were placed in MS medium for strong seedling growth, supplemented with sucrose 60g / L, agar 7g / L, pH 5.8, light 20000lx, photoperiod 14h / d, temperature 28℃, survival rate 83%.

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PUM

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Abstract

The invention researches a rapid propagation method of polyploidy mutation of cissampelos pareira. The method comprises steps as follows: obtaining of sterile pollen of the cissampelos pareira, induction of calluses, differentiation of the calluses, rooting induction and the like. A large number of polyploidy seedlings of the cissampelos pareira can be obtained rapidly with the method, and the method has the characteristics of high propagation rate, high purposefulness, simplicity in operation, low cost and the like.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of vine vine, belonging to the technical field of plants. Background technique [0002] tin vine, Cissampelos pareira L. Fangjike is a traditional medicinal material of the Dai nationality, and the Dai name is Yahonglong. Woody vines; branches slender, striated, usually densely pilose, rarely subglabrous, leaves papery, heart-shaped or nearly round, about 2-5 cm long and wide, often emarginate at the top , with convex apex, base often heart-shaped, sometimes nearly truncated, rarely, slightly rounded, hairy on both sides, often sparse above, densely below; palmate veins 5-7, slightly convex below; petiole often densely covered Plumber, shorter than blades. Male inflorescences are axillary, corymbose cymes, solitary or several in clusters, rachis and branches are thin, densely pubescent; male flowers; sepals 1.2-1.5mm long, with sparse and long hairs on the back; Corolla saucer-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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