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SiRNA inhibiting expression of gene S100A9 and application of siRNA

A technology of S100A9, gene expression, applied in the application field of inhibiting the migration function of nasopharyngeal carcinoma cell CNE1

Inactive Publication Date: 2015-01-21
GUANGXI MEDICAL UNIVERSITY
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Problems solved by technology

Therefore, designing S100A8 siRNA and S100A9 siRNA to reduce the expression of S100A8 / A9 in nasopharyngeal carcinoma cells CNE1, thereby inhibiting the migration of nasopharyngeal carcinoma cells, has important clinical significance. to report

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  • SiRNA inhibiting expression of gene S100A9 and application of siRNA
  • SiRNA inhibiting expression of gene S100A9 and application of siRNA
  • SiRNA inhibiting expression of gene S100A9 and application of siRNA

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Embodiment Construction

[0026] The present invention will be further described in detail below through specific embodiments and accompanying drawings.

[0027] (1) Design and synthesis of S100A9siRNA:

[0028] According to the principle of siRNA design, 3 pairs of siRNA sequences were designed for the mRNA sequence of S100A9 gene. The design steps are: (1) use the Gene database of NCBI to find the mRNA sequence of S100A9, and its ACCESSION in NCBI is NM_002965.3; (2) use the WI siRNA Selection Program (http: / / sirna.wi.mit.edu / ) design tool, designed 3 pairs of siRNA sequences for different positions in the coding region; (3) analyzed using NCBI's BLAST, the results showed that these 3 pairs of siRNAs would not be homologous to other gene sequences, and the sequences of the 3 pairs of siRNAs were as follows:

[0029] S100A9siRNA1:

[0030] Sense strand: 5'-GCAGCUGGAACGCAACAUA-3' (SEQ ID NO.1)

[0031] Antisense strand: 5'-UAUGUUGCGUUCCAGCUGC-3'(SEQ ID NO.2)

[0032] S100A9siRNA2:

[0033] Sense ...

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Abstract

The invention discloses a siRNA inhibiting expression of a gene S100A9 and an application of the siRNA and relates to the field of molecular biology. The siRNA inhibiting the expression of the gene S100A9 is characterized in that a sequence of a positive-sense strand is 5'-GCUUCGAGGAGUUCAUCAU-3' and a sequence of an antisense strand is 5'-AUGAUGAACUCCUCGAAGC-3'. The siRNA disclosed by the invention can be used for reducing expression of the gene S100A9, inhibiting expression of a gene MMP7 in a nasopharyngeal carcinoma cell CNE1 and degrading a migration function of the nasopharyngeal carcinoma cell, so that the siRNA can be applied to research of a medicine used for treating nasopharyngeal carcinoma.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to siRNA for inhibiting the expression of S100A9 gene and its application in inhibiting the migration function of nasopharyngeal carcinoma cell CNE1. Background technique [0002] Nasopharyngeal carcinoma (Nasopharyngeal carcinoma NPC) is a malignant tumor derived from nasal mucosal epithelial cells, more than 95% of which are undifferentiated carcinomas, with high malignancy, fast growth, and prone to lymph node or blood metastasis. Cancer patients all die from tumor metastasis rather than the primary tumor. In recent years, with the rapid development of molecular biology and technology, the occurrence and development of nasopharyngeal carcinoma and its biological behavior have made great progress. A number of studies have shown that S100A8 and S100A9 are involved in the occurrence and development of tumors. Some studies have used iTRAQ protein quantitative labeling combined with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00
Inventor 黄元姣闫琳琳
Owner GUANGXI MEDICAL UNIVERSITY
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