Application of CD82 / TIMP1 in preparation of medicine for inhibiting migration of human pancreatic cancer cells

A technology for cell migration and human pancreatic cancer, applied in the field of bioengineering, can solve problems such as lack of enzyme activity, achieve good economic and social benefits, good practicability, and the effect of inhibiting cancer cell migration

Inactive Publication Date: 2015-02-11
NANJING SENMU BIOTECH CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

CD82 inhibits cancer cell metastasis from multiple aspects, but it does not have enzymatic activity itself

Method used

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  • Application of CD82 / TIMP1 in preparation of medicine for inhibiting migration of human pancreatic cancer cells
  • Application of CD82 / TIMP1 in preparation of medicine for inhibiting migration of human pancreatic cancer cells
  • Application of CD82 / TIMP1 in preparation of medicine for inhibiting migration of human pancreatic cancer cells

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Embodiment Construction

[0014] In order to facilitate the understanding of those skilled in the art, the present invention will be further described below in conjunction with the accompanying drawings.

[0015] Human pancreatic cancer PANC-1 cell line was purchased from American ATCC Company; recombinant TIMP1 and CD82-LEL proteins were purchased from Beijing Yiqiao Shenzhou Biotechnology Co., Ltd.; cyclopamine (cyclopamine) was purchased from American Biomol International Lp Company; FITC-labeled ghost pen Cyclic peptides were purchased from Sigma, USA.

[0016] 1. PANC-1 cells were cultured in DMEM medium containing 10% fetal bovine serum, 100U / ml penicillin and 100U / ml streptomycin.

[0017] 2. Add recombinant TIMP1 to the culture medium containing 5% serum of poorly differentiated and highly migratory PANC-1 cells. 10uM) as a positive reference for the system.

[0018] 3. Add the CD82 interference group, and observe the cell migration at the two time points of 6 hours and 18 hours.

[0019] 4....

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Abstract

The invention discloses application of CD82 / TIMP1 in preparation of medicine for inhibiting migration of human pancreatic cancer cells, and belongs to the technical field of biological engineering. CD82 and TIMP1 are directly combined to trigger the intracellular transport and circulation of TIMP1, block cytoskeleton remodeling, reduce lamellipodia / filopodia projecting formation and inhibit cell viability and migration. TIMP1 enhances an Akt signaling pathway by CD82 on a pancreatic cancer cell membrane, blocks cytoskeleton remodeling, reduces lamellipodia / filopodia projecting formation, inhibits cell viability and migration, and has no effect on the growth of cancer cells. The deep explanation on a TIMP1 / CD82 axis as a new signaling pathway for inhibiting tumor metastasis helps us more clearly know and understand the process of cancer metastasis, and helps find a critical juncture of prevention and control of cancer metastasis.

Description

[0001] technical field [0002] The invention belongs to the technical field of bioengineering, in particular to the application of CD82 / TIMP1 in the preparation of drugs for inhibiting the migration of human pancreatic cancer cells. Background technique [0003] Cancer cell metastasis is still the main cause of cancer death. Cancer metastasis is a multi-step process, in which the first step is that cancer cells migrate from the primary site, then enter the blood circulation, grow and proliferate after reaching the remote parts of the body with the blood, and finally form metastases. A comprehensive understanding of the process of cancer metastasis, including the related molecular mechanisms, will help researchers to develop new cancer treatment drugs and therapies. Over the past few decades, more and more knowledge about the process of cancer metastasis has been gained with the gradual discovery of specific cancer metastasis suppressor genes. At the same time, the researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P35/04
Inventor 韩晓张洁心
Owner NANJING SENMU BIOTECH CO LTD
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