Pseudomonas strain and method for preparing composite surfactant by using same

A Pseudomonas strain and surfactant technology, which is applied in the field of Pseudomonas strain and its preparation of compound surfactants, can solve the problems of application field and application effect limitation, and achieve good surface activity effect

Active Publication Date: 2015-02-18
SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The microorganisms that have been found to produce biosurfactants include bacteria and yeast, but they on...

Method used

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  • Pseudomonas strain and method for preparing composite surfactant by using same
  • Pseudomonas strain and method for preparing composite surfactant by using same
  • Pseudomonas strain and method for preparing composite surfactant by using same

Examples

Experimental program
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Embodiment 1

[0021] Embodiment 1: Utilize Pseudomonas ( Pseudomonas sp. ) The fermentation broth of CCTCC NO: M2013570 should be applied to the water body, soil, pipeline equipment that needs to be treated, or used simultaneously with other microorganisms or compounds.

[0022]Activate the preserved strains by conventional methods, then insert them into the liquid seed medium, and cultivate them at 30-37°C and 150 r / min for 18-24 hours to prepare liquid seeds. ,; The obtained liquid seeds are transferred into a primary fermenter (inoculum size 5%), and the primary fermentation strain is obtained under the condition of a ventilation rate of 0.75-1.0vvm and a stirring speed of 160r / min; The first-grade fermentation strains are transferred into the second-grade fermentation tank (5% inoculum size), and the second-grade fermentation strains are obtained under the conditions of ventilation volume 0.75-1.0vvm, stirring speed 160r / min; the second-grade fermentation strains Then transfer it to a ...

specific Embodiment 2

[0028] Specific embodiment 2: utilize Pseudomonas ( Pseudomonas sp. ) The liquid strain of CCTCC NO: M2013570 is directly inoculated in the water body, soil, pipeline equipment that needs to be treated, or used simultaneously with other microorganisms or compounds.

[0029] Activate the preserved strains by conventional methods, then insert them into the liquid seed medium, and cultivate them at 30-37°C and 150 r / min for 18-24 hours to prepare liquid seeds. ; The obtained liquid seeds are transferred into a primary fermenter (inoculum size 5%), and the primary fermentation strain is obtained under the condition of aeration rate 0.75-1.0vvm, stirring speed 160r / min; Transfer the fermented strains into the secondary fermenter (inoculum size 5%), and prepare the secondary fermented strains under the condition of ventilation rate 0.75-1.0vvm, stirring speed 160r / min; The seed solution is applied to water bodies, soils, etc. polluted by crude oil and edible oil, or used simultaneo...

specific Embodiment 3

[0032] Specific embodiment 3: utilize Pseudomonas ( Pseudomonas sp. ) CCTCC NO: M2013570 slant strains are applied directly to the water body, soil, pipeline equipment to be treated, or used simultaneously with other microorganisms or compounds.

[0033] Activate the preserved strains according to the conventional method, then wash the bacterial lawn into 0.1mol / L phosphate buffer solution, add glass beads to shake and disperse. Adjust the bacterial concentration to 10 9 -10 10 After / mL, apply the seed buffer to crude oil, edible oil contaminated water, soil, etc., or use it simultaneously with other microorganisms or compounds.

[0034] Slant medium: glucose 16 g, peptone 8 g, yeast extract powder 0.1 g, agar 20 g, dilute to 1 L, pH 7.0-7.4.

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Abstract

The invention discloses a pseudomonas strain and a method for preparing a composite surfactant by using the same and relates to a microbial strain and a method for preparing an active agent. The strain is named as pseudomonas which is preserved in China Center for Type Culture Collection (CCTCCNOM) on November 13, 2013 and has a preservation number of CCTCCNO: M2013570; the invention discloses a pseudomonas CCTCCNO: M2013570 and an application thereof. The strain disclosed by the invention can produce the composite surfactant, namely, glycolipid and amino-acid-containing lipoids, during a fermentation process by virtue of a plurality of carbon sources; the strain, as well as the fermentation broth and surfactant extracts thereof have good application prospects in wastewater treatment, treatment of soil heavy metal pollution, preservation and bacteria inhibition of foods such as fruits and vegetables, washing and daily chemical products and the like. The synergistic effects of different biological surfactants can be given and the strain can play a role under various conditions.

Description

technical field [0001] The invention relates to a microbial strain and a method for preparing an active agent, in particular to a pseudomonas strain and a method for preparing a composite surfactant. Background technique [0002] Biosurfactants are metabolites with certain surface activity secreted by microorganisms in the metabolic process. In addition to the general functions of surfactants such as reducing surface tension, stabilizing emulsions and increasing foam, they also have the functions of chemically synthesized surfactants. It has the advantages of being non-toxic and biodegradable, and has broad application prospects. The microorganisms that have been found to produce biosurfactants include bacteria and yeast, but they all produce only one type of surface active substances, which limits their application fields and application effects to a certain extent. Contents of the invention [0003] The object of the present invention is to provide a kind of Pseudomonas...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12R1/38
CPCC12P1/04C12N1/205C12R2001/38
Inventor 张卉
Owner SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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