Application method of sexual attractant of neem tortrix
An application method, the technology of the small tortrix, applied in the direction of attracting pests, application, botany equipment and methods, etc., can solve the problems affecting the utilization value, pesticide residues, pest resistance, etc., and achieve significant social and ecological benefits , reduce population density, and protect natural enemies
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[0017] Below in conjunction with specific examples, the sex attractant of the neem tree moth of the present invention is further described.
[0018] 1. Extraction of sex pheromones
[0019] Take the virgin female moth of Neemia japonica, darken it, and cut off the sex gland of the female moth when it is observed that the female moth is in the state of mating call. Take the virgin female moth and freeze it in the refrigerator for 2 minutes, cut off the gland with ophthalmic surgical scissors, absorb the gland attachment liquid with filter paper, put the sex pheromone gland into a 1ml chromatographic sampling bottle with an insect needle, and completely soak the gland in Re-distill in n-hexane, let it stand at room temperature for 50 minutes, then use a 1ml glass syringe to absorb the soaking liquid, filter it through an organic membrane filter, and transfer it to another clean chromatography bottle (you can use a nitrogen concentrator to concentrate the filtrate if the number o...
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