Microbiological detection device and microbiological detection method

Abstract

The invention relates to the field of microculture and detection, and provides a microbiological detection device and a microbiological detection method, aiming at solving the problems of time consuming, high cost and poor biocompatibility and the like in the existing microbiological detection method. The microbiological detection device comprises a reaction chamber used for containing nutrient solution, a sensitive element and an organic thin-film field effect transistor, wherein the sensitive element is communicated with the reaction chamber, is contacted with the nutrient solution and is used for sensing pH value variation of the nutrient solution and producing an electric charge according to the pH value variation; and the organic thin-film field effect transistor is electrically communicated with the sensitive element and is used for determining whether microorganisms exist or not according to charge of the sensitive element, and kinds of the microorganisms are also determined. The organic thin-film field effect transistor is adopted as a detecting element, so that microorganism growth condition in the nutrient solution can be detected in real time according to variation of channel current of the transistor, a detection result can be rapidly obtained, and the microbiological detection method has the advantages that the cost is low and biocompatibility is good compared with the existing detection method.

Description

technical field [0001] The invention relates to the field of microorganism cultivation and detection, and specifically provides a microorganism detection device and method. Background technique [0002] Microorganisms widely exist in nature. The main detection method adopted in the prior art is microbial culture. During the culture process, samples are placed in an environment with controlled media composition and culture conditions to promote microbial growth. Currently, there are a variety of microbial culture devices on the market that can detect the presence of microorganisms in a sample within 12-48 hours and rule out any infectious microorganisms present within 5 days. In addition, the devices allow an additional 12-48 hours to characterize the microbes by subjecting the samples to secondary incubation and testing. However, the length of time required by the above-mentioned detection methods is totally unacceptable in applications where rapid detection results are r...

AI Technical Summary

Problems solved by technology

Specifically, the present invention aims to solve the problems that the existing microbial detection methods cannot perform real-time detection, the detection time is too long, the cost is high, the biocompatibility is poor, and the sensitivity is low.

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