A kind of quadruple probiotic preparation and its application

A technology of quadruple probiotics and preparations, applied in the application, lactobacillus, bifidobacteria and other directions, can solve the problem that bifidobacteria does not solve the problem of normal temperature preservation, and achieves the benefits of human health, promotion of proliferation, and high number of viable bacteria. Effect

Active Publication Date: 2016-10-05
HANGZHOU GRAND BIOLOGIC PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, judging from the probiotics products currently on the market, most of the products containing bifidobacteria have not solved the problem of storage at room temperature

Method used

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  • A kind of quadruple probiotic preparation and its application
  • A kind of quadruple probiotic preparation and its application
  • A kind of quadruple probiotic preparation and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Isolation screening, identification and cultivation of embodiment 1 bacterial strain

[0048] 1. Collection of strains

[0049]Bifidobacterium animalis and Enterococcus faecalis were isolated from healthy human intestines in Jianggan District, Hangzhou. The preservation number of Bifidobacterium animalis is CGMCC No.4521, and the preservation place is General Microbiology Center, Institute of Microbiology, Chinese Academy of Sciences, and the preservation date is December 29, 2010; the preservation number of Enterococcus faecalis is CGMCC0460.3, and the preservation place is It is the General Microbiology Center of the Institute of Microbiology, Chinese Academy of Sciences, and the preservation date is June 20, 2000; the preservation number of Lactobacillus casei is CGMCCNo. on June 29; the preservation number of Bacillus cereus strains is CGMCC0460.4, and the preservation place is the General Microbiology Center of the Institute of Microbiology, Chinese Academy of Sci...

Embodiment 2

[0063] The preparation of embodiment 2 bacterial powder

[0064] After activating and amplifying the culture of the four strains, the fermented fermentation broth of each strain was centrifuged at a speed of 10,000 rpm, and the clear supernatant was removed to obtain the sludge. Add the pre-killed protective agent to the bacteria slime and mix evenly with a strong mixer, then pre-freeze the bacteria slime in a refrigerator at -40°C--20°C overnight, and then freeze-dry. The freeze-drying temperature is -60°C, the vacuum degree is 10-30Pa, and the vacuum freezing time is 12-24h. After freezing, the freeze-dried cells were taken out, pulverized, passed through a 40-mesh sieve, vacuum-packed and stored at -80°C for later use.

Embodiment 3

[0066] This embodiment provides a kind of preparation method of quadruple probiotic capsule, the steps are as follows:

[0067] 1) Raw material pretreatment: according to the weight percentage of the product, respectively take 3% of Bifidobacterium animal powder, 3% of Lactobacillus casei powder, 1% of Enterococcus faecalis powder, 0.1% of Bacillus cereus powder, vitamin C 4%, lactose 9.20%, microcrystalline cellulose 35.75%, sucrose 14.50%, magnesium trisilicate 4.25%, aluminum hydroxide 2.25%, talcum powder 10.50%, and the rest are filled with starch.

[0068] 2) Preparation by mixing: pass sucrose powder and starch through a 50-mesh sieve and add to the batch mixer, then add the remaining auxiliary materials into the batch mixer in sequence, and finally add the mixed bacteria powder. Four kinds of freeze-dried bacteria powders and auxiliary materials were mixed in a mixer for 50 minutes and then discharged.

[0069] 3) Molding: use a capsule filling machine to put the samp...

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Abstract

The invention discloses a quadruple probiotic preparation and its application, belonging to the technical field of health food / medicine. The quadruple probiotic preparation provided by the present invention contains Bifidobacterium animalis, Lactobacillus casei, Enterococcus faecalis and Bacillus cereus, and the four probiotics account for 3.0%-8.0% and 3.0%-8.0% of the weight of the product respectively , 0.5%‑2.0% and 0.1%‑0.5%, other components are auxiliary materials. The quadruple probiotic preparation provided by the invention has the characteristics of high number of viable bacteria and is convenient for storage at room temperature. Each gram of the quadruple probiotic preparation provided by the invention contains more than 1ⅹ108 CFU of Bifidobacterium animalis and more than 1ⅹ108 CFU of Lactobacillus casei, more than 1ⅹ107 CFU of Enterococcus faecalis, and more than 1ⅹ106 CFU of Bacillus cereus. After storage at room temperature for 24 months, the number of viable bacteria of Bifidobacterium can reach 3.8×108CFU / g, that of Lactobacillus casei can reach 7.8×108CFU / g, and that of Enterococcus faecalis can reach 2.2×107CFU / g, the number of live bacteria of Bacillus cereus can reach 1.0×106CFU / g.

Description

technical field [0001] The invention relates to a quadruple probiotic preparation and its application, and belongs to the technical field of health food / medicine. technical background [0002] Probiotics refer to live bacterial preparations and their metabolites that can improve the ecological balance of the host's intestinal flora, improve the health level and health status of the host. Probiotics are acquired microorganisms that, when ingested in sufficient quantities, exert a beneficial health effect on the host. Therefore, probiotics have high application value in important fields closely related to human life such as industry, agriculture, medicine, food and feed. In recent years, a variety of probiotic live bacterial preparations have been developed at home and abroad. The basic guiding principle is to use strains of normal physiological flora of humans or animals, after screening and cultivation, to make various dosage forms of live bacterial preparations through var...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/135A23L3/3463A61K35/747A23L33/00
CPCA23L3/3454A61K35/742A61K35/744A61K35/745A61K35/747A23V2002/00A23V2400/125A23V2400/515A23V2250/5118A23V2250/628A23V2250/612A23V2250/51084A23V2250/70A23V2200/32A61K2300/00
Inventor 曹勇闫天文
Owner HANGZHOU GRAND BIOLOGIC PHARMA INC
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