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Pantoea agglomerans with molluscicidal activity and application thereof

A technology of Pantoea agglomerans and activity, applied in the field of microorganisms, can solve problems such as irreversible damage to the environment, and achieve the effects of low cost, simple preparation method, and environmental friendliness

Inactive Publication Date: 2015-04-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main molluscicides are chemical drugs, which are likely to cause irreversible damage to the environment

Method used

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  • Pantoea agglomerans with molluscicidal activity and application thereof
  • Pantoea agglomerans with molluscicidal activity and application thereof
  • Pantoea agglomerans with molluscicidal activity and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0014] The screening of a kind of Pantoea agglomerans CCTCC NO: M2014104 strain with molluscicidal activity of the present embodiment comprises the following steps:

[0015] 1. Preparation of culture medium:

[0016] 1) YEB liquid medium: beef extract 5.0g / L, yeast extract 1.0g / L, peptone 5.0g / L, MgSO 4 ·7H 2 O is 4g / L, sucrose is 5.0g / L, pH7.0~7.5;

[0017] 2) YEB solid medium: 15 g / L agar was added to the above YEB liquid medium to obtain a YEB solid medium.

[0018] 2. Screening steps:

[0019] 1) Under aseptic conditions, take fresh tea tree leaves, soak them in 75% ethanol for 1 minute, soak them in 0.1% mercuric chloride for 12 minutes, wash them with sterile saline for 3 times, and grind the leaves to obtain tissue fragments ;

[0020] 2) Inoculate the tissue minced in step 1) into 100 mL of YEB liquid medium, culture at 37° C. for 12 to 16 hours at a rotation speed of 180 rpm, and obtain a culture medium;

[0021] 3) by volume, the culture solution of step 2) was...

Embodiment 2

[0030] This embodiment is the determination of the molluscicidal activity of the bacteria CCTCC NO: M2014104:

[0031] 1. Preparation of molluscicide:

[0032] Inoculate the bacterium CCTCC NO: M2014104 in 100mL of YEB liquid medium, culture at 37°C for 24 hours at a speed of 180rpm to obtain seed liquid, transfer 1mL of seed liquid to 100mL of YEB liquid medium, and culture at 37°C After 12 to 16 hours, the rotation speed of the culture is 180 rpm, and the fermentation liquid is obtained, which is the biological molluscicide.

[0033] 2. Preparation of the molluscicide dilution: the molluscicide is prepared into a solution with a volume fraction of 10% with dechlorinated water, which is the molluscicide dilution.

[0034] 3. Determination of molluscicidal activity:

[0035] After the snails collected in the field were cultured at 25°C for 3 days, the snails with good vigor were selected and rinsed with dechlorinated water for 3 times, and the snails were divided into three ...

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Abstract

The invention discloses pantoea agglomerans (Pantoea agglomerans C4) with the molluscicidal activity and application thereof. The biological preservation number of the pantoea agglomerans is CCTCC NO: M2014104; bacteria with the preservation number of CCTCC NO:M2014104 are pantoea bacteria and are screened from tee leaves; and oncomelania can be rapidly and effectively killed. The pantoea agglomerans has the advantages that the new bacteria for killing the oncomelania is provided; the pantoea agglomerans can be used as a biological oncomelania killing agent directly; and compared with a traditional chemical oncomelania killing agent, the biological oncomelania killing agent prepared with the bacteria with the preservation number of CCTCC NO: M2014104 is simple in preparation method, low in cost and environment-friendly.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a pantoea agglomerans with molluscicidal activity and application thereof. Background technique [0002] Oncomelania is the only intermediate host of schistosomiasis, and its reproduction and distribution are closely related to the prevalence and spread of schistosomiasis. Therefore, suppressing snails and killing snails is considered an important way to prevent the spread of schistosomiasis. At present, the main molluscicides are chemical drugs, which are likely to cause irreversible damage to the environment. In recent years, the microbial community structure in plants has received extensive attention and research. Scholars at home and abroad have found through research that there are a large number of microorganisms in plants, and these microorganisms play an important role in the growth, development, and health of animals. Therefore, the present invention will The direction of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 叶爱华郭小双田胜尼袁艺丁洲应明张仪
Owner ANHUI AGRICULTURAL UNIVERSITY
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