A seedling raising method of Epimedium wushanense
A technology of epimedium and seeds, applied in the seedling field of Wushan Epimedium, can solve the problems of long natural dormancy time, unfavorable large-scale breeding, small population, etc., and achieve average germination time reduction, good market prospect, and germination index Improved effect
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Embodiment 1
[0037] Such as figure 1 As shown, a method for raising seedlings of Epimedium Wushanense in the present invention comprises step 1, collecting Epimedium Wushanii seeds. From May to June, the capsules of Epimedium Wushanense were collected in Longli, Qiannan Prefecture, Guizhou Province, and then the pericarp of the capsules were removed. The seeds of Epimedium Wushanense were stored in ziplock bags (at 10°C) for later use.
[0038] Step 2, variable temperature stratification of Epimedium Wushan seeds.
[0039] Pass the river sand through a 40-mesh square-hole standard sieve to remove stones and clods, wash away the soil in the river sand with clean water, and dry it for later use; before seed dressing, the sand is sterilized at 120°C for 3 hours, and after cooling to room temperature, Use distilled water to control the water content of river sand to 20wt% (add 20ml distilled water to 100g sand and mix well);
[0040] Use 1.0wt% sodium hypochlorite solution to sterilize Epime...
Embodiment 2
[0100] Embodiment 2: Seedling character observation
[0101] 1. Seedling transplantation
[0102] When investigating the number of germination, pick out the seeds whose radicle breaks through the seed coat, transfer them to 0.5% agar medium, and cultivate them in an incubator with 12h / 12h alternating light and dark at 20°C to observe the characteristics of the seedlings after different treatments.
[0103] 2. Seedling observation
[0104] Depend on Figure 4 It can be seen that not all treatments can obtain normal seedlings. Since fluridone is toxic to plants, when the concentration exceeds a certain level, it will cause stunted plant growth, such as cotyledons unable to break through the seed coat, delayed or absent heart leaf formation. In addition, high concentrations of fluridone can cause albinism of seedlings, hinder photosynthesis of plants, and eventually fail to form normal plants.
[0105] The Epimedium wushanense seeds treated by the control (F0GA0) can obtain no...
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