A seedling raising method of Epimedium wushanense

A technology of epimedium and seeds, applied in the seedling field of Wushan Epimedium, can solve the problems of long natural dormancy time, unfavorable large-scale breeding, small population, etc., and achieve average germination time reduction, good market prospect, and germination index Improved effect

CN104770099BInactive Publication Date: 2017-01-04CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2017-01-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a seedling growing method of Wushan epimedium, and belongs to the technical field of traditional Chinese herbal cultivation. The method comprises the following steps: step one, collecting and pre-treating Wushan epimedium seeds; step two, subjecting the processed Wushan epimedium seeds to a temperature-variable stratification treatment, wherein in the temperature-variable stratification treatment, the Wushan epimedium seeds are placed at a temperature of 10 DEG C for 12 hours and then placed at a temperature of 20 DEG C for 12 hours, and the two steps are carried out alternately; step three, soaking the Wushan epimedium seeds in a solution containing fluridone; step four, subjecting the Wushan epimedium seeds to a low-temperature (3-6 DEG C) stratification treatment. Through the method, the germination rate, germination potential, and germination index of Wushan epimedium seeds are all prominently improved, and the average germination time is obviously shortened. The operation is simple, the controllability is strong, the Wushan epimedium seed dormancy can be awaken efficiently, and normal seedlings can be obtained.
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Description

technical field

[0001] The invention belongs to the technical field of cultivation of Chinese medicinal materials, and in particular relates to a method for raising seedlings of Epimedium wushanense. Background technique

[0002] Epimedium wushanense T.S. Ying is a perennial broad-leaved plant of the genus Epimedium in the Berberidaceae family (Berberidaceae). Its medicinal part is the dry shoot of the whole plant. It is a species listed in the 2010 edition of the Pharmacopoeia of the People's Republic of China. , has the effects of aphrodisiac, osteoporosis, and rheumatism. In addition, its extracts are effective in anti-oxidation, anti-tumor, anti-aging, anti-atherosis, anti-depression, and enhancing immunity. In European and American countries, Epimedium is also used for home ornamental gardening crops.

[0003] Under natural conditions, the reproduction mode of Epimedium Wushanense is to rely on asexual reproduction of underground rhizomes and seed reproduction (sexual ...

Examples

Embodiment 1

[0037] Such as figure 1 As shown, a method for raising seedlings of Epimedium Wushanense in the present invention comprises step 1, collecting Epimedium Wushanii seeds. From May to June, the capsules of Epimedium Wushanense were collected in Longli, Qiannan Prefecture, Guizhou Province, and then the pericarp of the capsules were removed. The seeds of Epimedium Wushanense were stored in ziplock bags (at 10°C) for later use.

[0038] Step 2, variable temperature stratification of Epimedium Wushan seeds.

[0039] Pass the river sand through a 40-mesh square-hole standard sieve to remove stones and clods, wash away the soil in the river sand with clean water, and dry it for later use; before seed dressing, the sand is sterilized at 120°C for 3 hours, and after cooling to room temperature, Use distilled water to control the water content of river sand to 20wt% (add 20ml distilled water to 100g sand and mix well);

[0040] Use 1.0wt% sodium hypochlorite solution to sterilize Epime...

Embodiment 2

[0100] Embodiment 2: Seedling character observation

[0101] 1. Seedling transplantation

[0102] When investigating the number of germination, pick out the seeds whose radicle breaks through the seed coat, transfer them to 0.5% agar medium, and cultivate them in an incubator with 12h / 12h alternating light and dark at 20°C to observe the characteristics of the seedlings after different treatments.

[0103] 2. Seedling observation

[0104] Depend on Figure 4 It can be seen that not all treatments can obtain normal seedlings. Since fluridone is toxic to plants, when the concentration exceeds a certain level, it will cause stunted plant growth, such as cotyledons unable to break through the seed coat, delayed or absent heart leaf formation. In addition, high concentrations of fluridone can cause albinism of seedlings, hinder photosynthesis of plants, and eventually fail to form normal plants.

[0105] The Epimedium wushanense seeds treated by the control (F0GA0) can obtain no...