Acetylcholine esterase activity detection test paper strip based on silver nanoparticles

A technology of silver nanoparticles and cholinesterase, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problems of low sensitivity and achieve high sensitivity and portability. convenient effect

Inactive Publication Date: 2015-07-22
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the sensitivity of this method is low, and there is a certain dependence on the instrument. Therefore, a simple, fast, high-sensitivity, and instrument-independent detection method for the detection of cholinesterase activity is established for organophosphorus pesticides with multiple components and residues. Rapid detection has great significance

Method used

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Embodiment Construction

[0014] 1. Assembly of lateral flow test strips for detection of cholinesterase activity

[0015] The invention provides a test strip for detecting cholinesterase activity based on silver nanoparticles. The test strip includes a cardboard, one side of the cardboard is pasted with absorbent paper, a detection pad, a recovery pad and a sample pad sequentially from top to bottom, and adjacent pads are overlapped and connected at joints. Among them, the detection pad is a nitrocellulose membrane. Three detection lines are set up on the membrane from top to bottom, and they are respectively coated with silver nitrate aqueous solution with a concentration of 0.5, 0.3, and 0.3nM. Vacuum drying at ℃ for 1-2 hours; the reducing mat is glass fiber, sprayed with a HEPES solution of vitamin C with a concentration of 0.1M, and after spraying, the reducing mat is vacuum-dried at 37°C for 1 hour. The absorbent paper is absorbent filter paper or oil filter paper with strong water absorption c...

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Abstract

The invention relates to a cholinesterase activity detection test paper strip based on silver nanoparticles. The test paper strip comprises a paper board, wherein water absorbent paper, a detection pad, a reduction pad and a sample pad are sequentially adhered on one side of the paper board from top to bottom, and the adjacent pads are overlapped and connected at the joints; the detection pad is a cellulose nitrate film, three detection lines are arranged on the film from top to bottom, and the detection lines are respectively coated with silver nitrate water solutions with concentrations from high to low; and the reduction pad is glass fiber and is sprayed with a certain concentration of HEPES solution of vitamin C. The test paper strip can be used for rapid detection of activity of cholinesterase and has the advantages of simplicity and convenience in use, high sensitivity, capability of obtaining detection results without relying on analysis of instruments and the like. The detection sensitivity of the established detection test paper strip against active cholinesterase can achieve 0.01nM, so that a new method can be provided for detection of the activity of cholinesterase.

Description

technical field [0001] The invention relates to a lateral chromatography test strip for detecting cholinesterase activity based on silver nanoparticles. Background technique [0002] At present, the extensive use of chemical pesticides, especially organophosphorus pesticides, poses a greater threat to people's health and environmental safety, so the highly sensitive detection of organophosphorus pesticides has greater social significance. [0003] There are many kinds of organophosphorus pesticides, and the current detection methods, especially rapid detection methods, are difficult to realize the simultaneous detection of multiple components and residues of organophosphorus pesticides. Studies have found that organophosphorus pesticides are a class of compounds that can reduce the activity of cholinesterase, so people can use the change of cholinesterase activity to detect the residues of organophosphorus pesticides in agricultural products. For example, the Ellman colorim...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 刘凤权王利民王苏妍
Owner NANJING AGRICULTURAL UNIVERSITY
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