Polar body genome reconstruction ovum, preparation method and application thereof

A genome and egg technology, applied in the field of biomedicine, can solve problems such as offspring disease, and achieve the effects of easy operation, blocking cytoplasmic related diseases, and high sensitivity and specificity

Inactive Publication Date: 2015-08-12
FUDAN UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, research practice has shown that both metaphase chromosomes and prokaryotes are surrounded by a large number of mitochondria due to energy requirements, accounting for about 40% of the total mitochondria in the cytoplasm. During the process of nuclear replacement, these mitochondria The nucleus enters the new egg cytoplasm, so that the reconstructed egg after replacement still contains a certain amount of mutant mtDNA, which may pass through the "genetic bottleneck" and replicate and amplify in the offspring, accumulating to a certain threshold. still cause disease in offspring

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  • Polar body genome reconstruction ovum, preparation method and application thereof
  • Polar body genome reconstruction ovum, preparation method and application thereof
  • Polar body genome reconstruction ovum, preparation method and application thereof

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Embodiment Construction

[0062] In the following, the present invention will be further described in combination with specific examples of animal models, and compared with existing mitochondrial replacement technologies (ST, PNT) ( figure 1 A schematic diagram showing the research of the present invention). It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0063] one. Research Materials and Methods

[0064] (1) Research materials

[0065] New Zealand white mice simulate female mitochondrial disease patients in the research of the present invention. The hybrid mouse BDF1 of C57BL / 6 and DBA2 simulates healthy females donating oocytes in the study of the present invention. ICR mice, used as surrogate mothers in the present invention.

[0066] (2) Research methods

[0067] 1. Feasibility analysis of PB1 and PB2 in the treatment of maternal mitochondrial diseases

[0068] (1) Comparison o...

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Abstract

The invention belongs to the bio-medical field, relates to a polar body genome and an application thereof, and particularly relates to a polar body genome reconstruction ovum, a preparation method and an application thereof. In the invention, according to a characteristic that a polar body not only contains female DNA being same as that in egg cell cytoplasm but also carries little even no cytoplasm substances (mitochondria), the polar body genome reconstruction ovum and an application thereof in preparation of a material for preventing mitochondria maternal genetic diseases are provided in the invention. An animal model test proves that the reconstruction ovum and progenies thereof are free of mitochondria DNA of patients, so that the reconstruction ovum can effectively stop cytoplasm-related diseases and can actively and completely prevent the mitochondria maternal genetic diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a polar body genome and its use, in particular to a polar body genome restructured egg and its preparation method and use, especially its use in preparing materials for treating maternal mitochondrial genetic diseases. Background technique [0002] According to research reports, mitochondrial DNA (mitochondrial DNA, mtDNA) is another genome independent of the nucleus extrachromosomal, known as human chromosome 25, and its genetic characteristics are non-Mendelian inheritance, also known as maternal inheritance ), that is, the mother passes on mtDNA to her sons and daughters, but only the daughter can pass on her mtDNA to the next generation. Mitochondrial disease (mitochondriopathy) refers to a group of multi-system diseases caused by defects in mitochondrial metabolic enzymes caused by genetic defects, resulting in ATP synthesis disorders and insufficient energy sources. Among them, mt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/873
CPCA61K35/54A61P21/04C12N5/0604C12N5/0609C12N5/16C12N15/873C12N2510/00
Inventor 沙红英朱剑虹曹云霞王天纪冬梅
Owner FUDAN UNIV
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