Method and system for in vitro developmental toxicity testing
一种体外测试、毒性的技术,应用在生物测试、测量装置、胚胎细胞等方向,能够解决无法形成心肌细胞等问题
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[0071] Materials and methods
[0072] cell patterning
[0073] In order to generate mechanical stress gradients, circular or square models with two different sizes (785,000 μm) were designed using AutoCAD software. 2 or 196250μm 2 ) Matrigel matrix. Then make a polydimethylsiloxane (PDMS) template by using a laser cutter (Universal Laser System) to cut the pattern of the design shape on a 127 μm thick PDMS sheet (Specialty Silicone Products Inc.) and It was performed in conjunction with a laser cut 2 mm thick PDMS liner. For cell patterning, the PDMS template was first sealed into a 60 mm Petri dish by adding 200 μl of 70% ethanol and dried in a tissue culture incubator (hood), then passed through with Matrigel TM Solution incubated for at least 1 hour to coat Matrigel TM (BD Biosciences) matrix ( figure 1 ). Thereafter cells were seeded at a density of 4 million cells / ml. , after incubation for 1 hour to allow cells to attach, the template and excess non-attached ce...
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