Method and system for in vitro developmental toxicity testing

一种体外测试、毒性的技术，应用在生物测试、测量装置、胚胎细胞等方向，能够解决无法形成心肌细胞等问题

Active Publication Date: 2015-08-26
AGENCY FOR SCI TECH & RES
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Problems solved by technology

It is therefore not compatible with the intrinsic properties of hESCs, which cannot form cardiomyocytes as easily as mouse ES cells

Method used

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  • Method and system for in vitro developmental toxicity testing
  • Method and system for in vitro developmental toxicity testing
  • Method and system for in vitro developmental toxicity testing

Examples

Experimental program
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Embodiment

[0071] Materials and methods

[0072] cell patterning

[0073] In order to generate mechanical stress gradients, circular or square models with two different sizes (785,000 μm) were designed using AutoCAD software. 2 or 196250μm 2 ) Matrigel matrix. Then make a polydimethylsiloxane (PDMS) template by using a laser cutter (Universal Laser System) to cut the pattern of the design shape on a 127 μm thick PDMS sheet (Specialty Silicone Products Inc.) and It was performed in conjunction with a laser cut 2 mm thick PDMS liner. For cell patterning, the PDMS template was first sealed into a 60 mm Petri dish by adding 200 μl of 70% ethanol and dried in a tissue culture incubator (hood), then passed through with Matrigel TM Solution incubated for at least 1 hour to coat Matrigel TM (BD Biosciences) matrix ( figure 1 ). Thereafter cells were seeded at a density of 4 million cells / ml. , after incubation for 1 hour to allow cells to attach, the template and excess non-attached ce...

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Abstract

A method and system of in vitro developmental toxicity testing comprising the steps of micropatterning an extracellular matrix; growing embryonic stem cells on the micropatterned extracellular matrix in the presence of mesoendermal induction and testing for change of the geometrical mesoendoderm structure in the presence or absence of a test compound wherein (1) a decrease in mesoendodermal differentiation and / or (2) a change in morphology of the geometrical mesoendoderm structure in the presence of the test compound compared to cells in the absence of the test compound indicates that the test compound is a developmental toxic agent.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of Singapore Patent Application No. 201207242-7, filed 28 September 2012, the entire contents of which are incorporated herein by reference. technical field [0003] The present invention relates to methods and systems for developmental toxicity testing. Background technique [0004] The following discussion of the background of the invention is intended to aid in the understanding of the present invention. It should be understood, however, that this discussion does not acknowledge or admit that any of the material referred to was published, known, or was part of the common general knowledge in any jurisdiction as at the priority date of the application. [0005] Birth defects are one of the leading causes of newborn death worldwide 1、2、3 , which can also lead to long-term disability and disease. Malformations of fetal development can be attributed to genetic conditions or environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735G01N33/48
CPCG01N33/5014G01N33/5026G01N33/5044G01N33/5073
Inventor H·于Y-C·杜J·邢
Owner AGENCY FOR SCI TECH & RES
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