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A detection of cervical cancer marker-foxp3 autoantibody epitope amino acid sequence and application

An autoantibody, cervical cancer technology, applied in the field of immunology, can solve the problems of low sensitivity and poor specificity, and achieve the effect of high sensitivity and high specificity

Active Publication Date: 2018-05-25
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported antibody detection methods have low sensitivity and poor specificity, and the false negative rate can be as high as more than 50%.

Method used

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  • A detection of cervical cancer marker-foxp3 autoantibody epitope amino acid sequence and application
  • A detection of cervical cancer marker-foxp3 autoantibody epitope amino acid sequence and application
  • A detection of cervical cancer marker-foxp3 autoantibody epitope amino acid sequence and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Kit preparation

[0041] 1 Reagents See Tab.2-9 for reagent preparation.

[0042]

[0043]

[0044]

[0045]

[0046]

[0047]

[0048]

[0049]

[0050] 2 operations

[0051] (1) Coating: the working antigen and reference antigen are diluted to the working concentration with coating solution, and coated on the microtiter plate,

[0052] overnight at 4°C.

[0053] (2) Add plasma (primary antibody): Wash the ELISA plate 3 times with washing buffer, dilute the plasma to an appropriate concentration with the analysis solution, generally 1:100-1:500, 100µl per well, incubate at 37°C or room temperature 1.5h;

[0054] (3) Secondary antibody incubation: wash with washing buffer for 3 to 5 times, dilute the secondary antibody standard solution IgG with the analytical solution, add 100µl to each well, and incubate at 25°C / room temperature for 1h;

[0055] (4) Color development: wash with washing buffer 3 times, add 100 µl of substrate color developme...

Embodiment 2

[0058] Detection of FOXP3 auto IgG antibody in patients with cervical cancer

[0059] 1 Sample collection: 407 female patients diagnosed with cervical tumors for the first time were collected, including 204 cases of benign cervical tumors and 203 cases of malignant tumors. All serum samples had not undergone any anti-cancer treatment before collection, and had comprehensive clinical data and information. At the same time, 154 healthy control samples were recruited. Clinical interviews and imaging examinations ruled out the possibility of cervical cancer. The healthy group and the tumor group were matched in gender and age and were comparable ( P >0.05).

[0060] 2 Test results: From Tab.10-11, it can be seen that the area under the ROC curve (AU) of the IgG antibody to the FOXP3 antigen polypeptide in the plasma of patients with cervical cancer was 0.721, the sensitivity was 21.6%, and the specificity was 90%. The expression of FOXP3 autoantibody in the peripheral blood of...

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Abstract

The invention provides an epitope polypeptide for detecting a cervical cancer tumor marker-FOXP3 autoantibody and an application thereof, belongs to the technical field of immunology, and provides an antigen amino acid sequence of an immune response regulating gene FOXP3 autoantibody. The corresponding specific autoantibody in plasma of cervical cancer benign and malignancy patients is detected by applying an FOXP3 epitope polypeptide antigen, and the autoantibody can be used as a cervical cancer tumor marker for evaluating the risk factor of cervical cancer occurrence. The antigen epitope polypeptide and the antibody thereof can be used for preparation of cervical cancer early-diagnosis reagents and development of targeted drugs for treating cervical cancer.

Description

technical field [0001] The invention belongs to the technical field of immunology, and is a basis for preparing reagents for early diagnosis of cervical cancer and developing targeted drugs for treating cervical cancer. Background technique [0002] Cervical cancer is one of the most common gynecological malignancies, which seriously threatens women's health. According to the statistics of the World Health Organization, its incidence rate is second only to breast cancer, and it is increasing year by year and becoming younger. There are about 466,000 new cases of cervical cancer every year, 80% of which occur in developing countries, and about 270,000 deaths. There are about 100,000 new cases in my country every year, accounting for nearly 20% of the total number of new cases in the world. In recent years, there has been an increase in regions and an earlier age of onset. It is urgent to identify and discover serum tumor markers for early diagnosis of cervical cancer in orde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47G01N33/574
CPCC07K14/47C07K14/4748G01N33/574G01N33/57411
Inventor 刘林林
Owner JILIN UNIV
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