A kind of breeding method of improved tea tree varieties with anti-tumor function

A kind of functional and tea tree technology, which is applied in the field of breeding of improved tea tree varieties with anti-tumor function, to achieve the effect of effectively inhibiting the growth of cancer cells

Active Publication Date: 2016-06-08
福建春伦集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] To sum up, there are no breeding methods for improved tea tree varieties (excellent germplasm, excellent strains, and excellent varieties) that can efficiently inhibit tumors in vitro and in animals at home and abroad, as well as the types and quantities of active ingredients that must be contained in tea leaves. patents and publications

Method used

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  • A kind of breeding method of improved tea tree varieties with anti-tumor function
  • A kind of breeding method of improved tea tree varieties with anti-tumor function
  • A kind of breeding method of improved tea tree varieties with anti-tumor function

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Experimental program
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Embodiment 1

[0026] Experimental material: Tumor-suppressing function tea-1, its breeding method: use the natural hybrid offspring line "DC-1" with high content of Fujian tea polyphenols (the content of four components reaches the standard-EGCG, C, caffeine and catechin total amount) as the female parent, "Eight Immortals" (ECG up to the standard) and the geographically distant hybrid line "1005" (EGCG and ECG up to the standard), these two lines are used as the male parent for multiple male parent pollination, and the tea fruit is sown after maturity Seedling cultivation, remove the terminal buds when the seedling height is 25cm, and transplant and plant the seedlings with a height of 35cm according to the female parent species one by one from November to February of the next year; The content of biologically active ingredients in the dry weight of fresh leaves of tea trees: in the content of the basic active ingredients of tea trees (in the dry weight of tea leaves: more than 20% tea poly...

Embodiment 2

[0028] The breeding method is the same as that in Example 1, except that the species whose total catechin content, EGCG content, catechin C content, and ECG content reach the standard are selected as the female parent, and the breed whose catechin C content, ECG content, and caffeine content reach the standard The variety is the male parent, and the "Tumor-suppressing Functional Tea-2" with five components reaching the target was selected.

Embodiment 3

[0029] Embodiment 3: Anti-tumor experiment in vitro

[0030] Experimental materials: "Tumor-suppressing function tea-1" obtained in Example 1; ordinary tea leaves, provided by the Agricultural Products Quality Research Institute of Fujian Agriculture and Forestry University. The contents of caffeine, catechin and their monomers in the two kinds of tea are shown in Table 1-1.

[0031] Table 1-1 Caffeine and catechin and their monomer content (%)

[0032]

[0033] Other drugs: cisplatin water injection, produced by Faulding; epirubicin, produced by Pfizer Pharmaceuticals (Wuxi) Co., Ltd.; etoposide, produced by Qilu Pharmaceutical Co., Ltd.; 5-fluorouracil injection, produced by Shanghai Xudong Haipu Pharmaceutical Co., Ltd. Industry Co., Ltd.; Gefitinib, AstraZenecaUKLimited (UK); Gleevec, NovartisPharmaSteinAG (Novartis Pharmaceuticals); EGCG monomer (purity 95%), purchased from Sigma.

[0034] Cell lines: human liver cancer HepG2 cell line, human ovarian cancer SK-OV-3 c...

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Abstract

The invention belongs to the field of tea tree germplasm resources, and in particular relates to a method for breeding improved tea tree varieties with tumor-suppressing functions. The method adopts geographical distant hybridization, and the offspring of the hybridization measure the content of biologically active components in the dry weight of fresh leaves of tea trees. On the basis of screening the content of basic active components of tea trees, the total catechin content is ≥ 22%, and the EGCG content is ≥ 12%. The germplasm with catechin C content ≥ 2%, ECG content ≥ 3.5% and caffeine content ≥ 4.5% is a good variety of tea tree with anti-tumor function. The tea produced from this improved variety is the tumor-suppressing function tea. The tea can effectively inhibit liver cancer, ovarian cancer, cervical cancer, lung cancer, brain astroglioblastoma, breast ductal cancer, prostate cancer, leukemia cancer, gastric cancer, colon cancer, skin squamous cell carcinoma and pancreatic cancer in vitro. The growth of 13 kinds of human tumor cells can effectively inhibit the growth of transplanted tumors of human liver cancer cells in animals.

Description

technical field [0001] The invention belongs to the field of tea tree germplasm resources, and in particular relates to a method for breeding improved tea tree varieties with tumor-suppressing function. Background technique [0002] Modern scientific research shows that tea has pharmacological and health functions such as anti-oxidation, anti-tumor, anti-aging, anti-virus, anti-bacterial, lowering blood pressure, blood fat and blood sugar. Tea contains more than 400 kinds of organic compounds and more than 15 kinds of inorganic minerals. Among them, the main anti-tumor functional components are tea polyphenols and their oxidation products, caffeine, tea pigment and theanine. [0003] The main component of tea polyphenols is catechin, which is composed of several monomers, such as epicatechin gallate (ECG) and epigallocatechin gallate (EGCG) in ester catechins. The content of catechin is relatively high, accounting for about 75% of the total catechins, and its pharmacologica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A23F3/06A23L33/00A61K36/82A61P35/00A01H5/12A01H6/00
CPCA23F3/00G01N33/5097A23L33/105A61K36/82A01H5/12A61P35/00A01H6/00
Inventor 郑金贵林金科杨江帆陈团生廖素凤刘江洪林用松凃良剑黄学敏梁建斌吴亮宇陈源林文磊陈荣冰
Owner 福建春伦集团有限公司
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