33results about How to "Efficient anti-tumor effect" patented technology

The invention discloses an efficient antineoplastic lucid ganoderma extract. Supercritical CO2 extraction is implemented on one of the following raw materials: a lucid ganoderma fruiting body, a mixture of the lucid ganoderma fruiting body and edible grease, and a mixture of the lucid ganoderma fruiting body and a grease-enriched edible material; in the extracting process, natural grease in the raw material is taken out by the CO2 extraction under a supercritical state; the natural grease, as a natural entrainer and a carrier, can entrain and extract active materials, in particular triterpenoids, in the lucid ganoderma fruiting body, and the active materials are collected by a separation kettle. The extract disclosed by the invention is rich in ganoderma triterpene, as an efficient antineoplastic substance, in the lucid ganoderma fruiting body, and the preparation process is simple; through skilful specific setting of extracting method and active material carrier, extraction and enrichment process of the active material, namely preparation process of product, is completed in one step; in the lucid ganoderma extract, triterpenoids are rich and high in total content, and properties are stable; and the processing course is free from introduction of secondary pollutants and is safe to eat.

The invention provides anti-tumor targeting engineering bacteria, a bacterial agent and a method for preparing the bacterial agent. The anti-tumor targeting engineering bacteria contain an anti-tumor secretory expression vector pLAC-HA, and anti-tumor targeting engineering bacteria are named Escherichia coli LMA1 through classification. The integration expression vector used for construction is pLAC, and the cut Plac constitutive expression promoter is used to reinforce the expression of small peptides Ehp. The invention also discloses the method for preparing an anti-tumor targeting engineering bacterial agent. Animal test results show that the anti-tumor targeting engineering bacteria have the quite high targeting property on tumors, but do not have obvious adverse influence on normal lab mice, and the bacterial agent prepared by the anti-tumor targeting engineering bacteria is a medicament for effectively treating tumors.

The invention belongs to the biotechnical field, and concretely relates to a tumor necrosis factor family cell apoptosis protein fusion protein, and a preparation method and a use thereof. The fusion protein is composed of annexin, a connecting peptide and a tumor necrosis factor family cell apoptosis protein, and coding gene of the fusion protein is constructed through cloning. The tumor necrosis factor family cell apoptosis protein fusion protein has a substantial enhanced cell apoptosis induction effect, can induce the apoptosis of tumor cells insensitive to the cell apoptosis, and can reduce the protein administration dosage needed by the obtaining of the treatment effect.

The invention discloses a multi-stage liver-targeted intelligent nano drug delivery system, and belongs to the field of biological materials. The nano drug delivery system comprises hydrophobic dendrimers, hydrophilic natural macromolecular polysaccharides, active targeting groups and hydrophobic drug molecules. According to the nano drug delivery system provided by the invention, the hydrophobic dendrimers are linked to the hydrophilic natural macromolecular polysaccharides by virtue of environment-responsive chemical bonds, so that amphipathic molecules are formed; then, hydrophobic drugs are encapsulated in hydrophobic cavities of nanoparticles formed by the amphipathic molecules under hydrophilic and hydrophobic actions as well as a [pi]-[pi] stacking action; and the active targeting groups, which have a tumor specific recognition function, are linked to the natural macromolecular polysaccharides by virtue of stable covalent bonds. According to the drug delivery system, intelligent nanoparticles having an environment-responsive performance can be formed through self-assembling; the drug delivery system is good in biocompatibility and biodegradability, multi-stage liver targeting can be achieved, and the drug delivery system is applicable to delivery of such hydrophobic molecules as antineoplastic drugs, fluorescent molecules, photosensitizers and the like.

The invention relates to an injectable composite hydrogel loaded with a vascular disrupting agent and near-infrared optothermal response nanometer particles and applications in resisting tumor. The composite hydrogel comprises a hydrogel substrate material, as well as the vascular disrupting agent and the near-infrared optothermal response nanometer particles loaded in the hydrogel substrate material. The prepared nanometer composite hydrogel has the sol-gel converting behavior realizing syringeability and thermal hysteresis. Under the illumination of near-infrared light, the hybridized hydrogel has the excellent optothermal property, and meanwhile, the curative effects of the vascular disrupting agent are improved by enhancing the permeability of the tumor vessels. The hybridized hydrogeladopting the combined treatment of the vascular disrupting agent and the optothermal function has good safety and curative effects in resisting tumor.

The present invention belongs to the technical fields of biomedical medicine and nano-medicine, and relates to a stem cell tumor targeting system with internal nano prodrug and a preparation method thereof. The method uses stem cells as cell carriers to prepare an anti-tumor targeting system with general formula of Stem cells-(RGD-PPCD)n through endocytosis and inner load of nano prodrug. The nano prodrug has the characteristics of targeting, sustained release and acid sensitivity release; and the stem cell carrier can remotely target primary lesion and metastasis of tumor. In vitro experimental results show that the stem cells after drug load can retain the proliferation capacity and tumor migration characteristics; and the constructed system is stable to ensure that the drug stays in the form of nano-prodrug for several days in the cells, and can slowly release the drug. In vivo experimental results show that the constructed stem cell targeting system can significantly prolong the survival time of tumor-bearing animals and can maintain normal neurobehavioral characteristics of tumor-bearing animals in comparison with the original drug and nano prodrugs, so as to play the efficient, secure and long-lasting anti-tumor effect.

The invention belongs to the technical field of biological genetic engineering and particularly discloses design, a preparation method and pharmaceutical application of a tumor targeting tumor necrosis factor related apoptosis ligand variant. The tumor targeting tumor necrosis factor related apoptosis ligand variant is fusion protein composed by ligand, connecting peptide and tumor necrosis factor related apoptosis ligand of CD13 and is produced through cloning and constructing of coding genes of the variant, soluble recombination expression and simple separation and purification in a genetic engineering method. The variant generated by the preparation method of the tumor targeting tumor necrosis factor related apoptosis ligand variant has good tumor tissue targeting property, remarkably enhances antitumous effect, can reduce protein administration dosage required for obtaining therapeutic effect, improves bioavailability, lowers treatment cost, and eliminates potential toxic and side effect of the tumor necrosis factor related apoptosis ligand. Simultaneously, the manufacturing method of the tumor targeting tumor necrosis factor related apoptosis ligand variant provides an expression method for producing soluble high-polymer form content and a simple separation and purification process.

The invention discloses an orthoester 5-fluorouracil prodrug molecule. The orthoester 5-fluorouracil prodrug molecule is N-5-fluorouracil1-ethyl-2-carbonalkoxy-(1,3) dioxolane-4-methylformamide havinga structure as shown in formula (I) described in the specification, wherein n=2-32 and m=n+2. The invention further discloses a preparation method of the abovementioned prodrug molecule and a nanoparticle prepared therefrom and an application thereof. The orthoester 5-fluorouracil prodrug molecule, the preparation method and the nanoparticle and the application thereof have the advantages that the nanoparticle prepared by the orthoester 5-fluorouracil prodrug molecule not only has the advantages of a small molecule prodrug but also is endowed with the advantage of a nano drug delivery system(that is a drug carrier), the prodrug nanoparticle not only has excellent acid degradation property and reduced tumor cytotoxicity and realizes passive targeting enrichment of a drug in a tumor, the nanoparticle has high drug loading capacity because the drug is used as a main part of the carrier, and the nanoparticle is capable of loading other antitumor drugs and achieves the effect of cooperatively treating the tumor and thus has good application prospects in the field of tumor treatment.

A transferring-polyethanediol-medicine molecule composition for the target therapy of tumor is disclosed. It features the combination of transferring and polyethanediol to make it has both active and passive target functions. Its advantages are long semi-life and high accumulation degree in tumor tissue. It can be used to prepare the antineoplastic medicines.

The invention relates to a polyethylene glycol-dipeptide-antitumour drug complex and use thereof. The invention is characterized in that cathepsin B sensitive dipeptide is adopted to couple PEG and antitumour drug, so that not only modification advantage of PEG is remained, but also specificity degradation characteristic of the cathepsin B sensitive dipeptide at tumour is fully played, and influence of PEG modification on drug activity can be overcome, thus being a better tumour therapy solution.

The invention discloses application of adenosine in preparing a medicament for treating tumors. In the invention, the adenosine in the field of tumor treatment is applied. In the application, injections, sustained-release preparations or targeting preparations can be prepared from the adenosine. Standard substance anti-tumor effect contrast proves that: when reaching certain concentration in vitro or in vivo, the adenosine participating in human metabolism has remarkable effects of tumor cell apoptosis and resisting tumor angiogenesis. The application has the advantages that: compared with other anti-tumor medicaments, the adenosine has efficient, broad-spectrum and low-toxicity anti-tumor effect, can be used in chemotherapy of gastric cancer, lung cancer, liver cancer, colon cancer, breast cancer and reproductive system and other malignant tumors, has remarkable anti-tumor effect, and does not have bone marrow suppression and vital organ injury by conventional dosage.

An injection of water-soluble 17-allylamino-17-demethoxy geldanamycin (17-AAG) for treating multiple kinds of cancers is prepared from 17-AAG and polyethanediol for increasing the solubility of 17-AAG in water.

The invention discloses a phospholipase A2 sensitive glycerin skeleton anti-tumor prodrug of which a molecular structure is a glycerin skeleton including a long-chain alkyl ether at a 1st position, a conjugated linoleoyl at a 2nd position and a phosphoryl nucleoside at a 3rd position. Because that a tumor tissue can highly express phospholipase A2, the glycerin skeleton anti-tumor prodrug has a tumor environmental specificity and can release a plurality of components which have activities on tumor cells at the position of the tumor. The components work in combination so that a high-efficient anti-tumor effect is achieved. An active compound of a nucleoside-type anti-tumor drug is selected from cytosine arabinoside, gemcitabine, capecitabine, fludarabine and derivatives thereof. The glycerin skeleton anti-tumor prodrug can be prepared into following high-dispersing preparations: a liposome, a nonionic surfactant vesicle, nano particles, a nano emulsion or a self-assembling transmission system.

The invention relates to the technical field of traditional Chinese medicine preparation and in particular relates to a traditional Chinese medicine preparation for treating breast cancer and a preparation method thereof. Aiming at solving the technical problems, a new option is provided for treating the breast cancer. The invention discloses the traditional Chinese medicine preparation for treating the breast cancer; the traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 3000 to 60000 parts of taxus chinensis bark, 100 to 2000 parts of radix ginseng rubra and 200 to 4000 parts of liquorice root. The invention further provides the preparation method of the traditional Chinese medicine preparation for treating the breast cancer; the preparation method comprises the following steps: step 1, preparing taxus chinensis extract; step 2, preparing radix ginseng rubra extract and liquorice root extract; step 3, preparing the preparation. According to the method of the preparation, provided by the invention, a traditional common water technology is changed and harmful substances and toxins, which easily cause chronic diseases including hypertension, hyperlipidemia and hyperglycemia and endanger livers, in water are removed, so that the content and the medicinal effect of various medicine effective components are improved. The preparation provided by the invention is prepared from various natural medicines and has essential herbal medicines, so that the clinical dosage is greatly reduced; heavy burdens and untoward effects, on stomachers, the livers, kidneys, intestines and the like, are avoided, and the medication safety and the medication effectiveness are greatly improved.

The invention provides a medicine or composition for resisting colorectal cancer. The present invention finds for the first time that overexpression of specific sequence small molecule RNA (such as ath-miR158a or its variants) can significantly inhibit the proliferation of colorectal cancer cells and promote the apoptosis of colorectal cancer cells during the occurrence and development of colorectal cancer , so as to play an efficient anti-tumor effect, which is of great significance to the treatment of colorectal cancer tumors.

The invention relates to the technical field of medicines, in particular to a medicine composition, and a preparation method and application thereof. The medicine composition provided by the invention comprises at least two of an immune checkpoint inhibitor, a medicine for degrading the extracellular matrix of a tumor tissue and a small-molecule inhibitor, wherein the immune checkpoint inhibitor and the medicine for degrading the extracellular matrix of the tumor tissue carry related genes through a carrier and can be specifically expressed and released in a tumor microenvironment; and the purpose of treating cancers can be realized by combination with the small-molecule inhibitor. Researches show that a tumor inhibition effect can be improved by the combination of the immune checkpoint inhibitor, the medicine for degrading the extracellular matrix of the tumor tissue and the small-molecule inhibitor and is better than the tumor inhibition effect obtained by independently utilizing the above three components or utilizing any two of the above three components.

The invention provides a graphene oxide drug carrier and its preparation method and application. The preparation method of the graphene oxide drug carrier is as follows: (1) adding polyethylene glycol diamine to the dispersion liquid of graphene oxide, and adding polyethylene glycol diamine in the activator Under the action of the amide condensation reaction to obtain GO-PEG; (2) GO-PEG is dissolved in a solvent to configure a GO-PEG dispersion, add oxidized sodium alginate, and perform a Schiff base reaction under the action of an activator; the reaction ends After purification and drying, the graphene oxide drug carrier GO‑PEG‑OSA was obtained. Disperse GO-PEG-OSA in a solvent to form a GO-PEG-OSA dispersion, add the drug solution, stir for 10-20 hours, and centrifuge to obtain nano-medicine. The drug carrier and nanomedicine of the present invention are sensitive to pH and light and heat, have "pH / warm" dual intelligent response release characteristics, have excellent biocompatibility, stability and water solubility, and can efficiently and quickly carry drugs into lesions.

The invention provides a medicine or a composition for resisting colorectal cancer. It is first discovered by the invention that in the colorectal cancer generation and development process, overexpression of specific sequence small molecule RNA (such as ath-miR158a or variants thereof) can obviously inhibit proliferation of colorectal cancer cells and promote apoptosis of the colorectal cancer cells, so that an efficient anti-tumor effect is achieved, and the application has important significance in treatment of colorectal cancer tumors.

The invention discloses a synthesis method and application of an miRNA-carrying VB12 binding type nano-composite. The synthesis method comprises the following steps: mixing and dissolving a PLGA-PEG nano-composite, vitamin B12, EDC and DMAP in an anhydrous solvent A, and performing stirring for reaction at 15-40 DEG C; and after the reaction is finished, performing dialyzing, and removing the solvent to obtain the vitamin B12 combined nano-composite. The synthesis method has the advantages of mild preparation conditions, cheap required raw materials, convenient operation, simple process, highefficiency and practicability. The prepared nano-composite optimizes nucleic acid drugs from three aspects of water solubility, stability and tumor targeting, endows the nucleic acid drugs with efficient and low-toxicity anti-tumor effects, and has a wide application prospect in the field of tumor treatment.

The invention belongs to the field of biological technology, and specifically discloses a tumor necrosis factor family cell apoptosis protein fusion protein, a preparation method and application thereof. The fusion protein is composed of annexin, connecting peptide and tumor necrosis factor family cell apoptosis protein, and the coding gene of the fusion protein is constructed by cloning. The tumor necrosis factor family cell apoptosis protein fusion protein has significantly enhanced effect of inducing apoptosis, can induce apoptosis of tumor cells insensitive to cell apoptosis, and can reduce protein dosage required for obtaining therapeutic effect.

The invention provides anti-tumor targeting engineering bacteria, a bacterial agent and a method for preparing the bacterial agent. The anti-tumor targeting engineering bacteria contain an anti-tumor secretory expression vector pLAC-HA, and anti-tumor targeting engineering bacteria are named Escherichia coli LMA1 through classification. The integration expression vector used for construction is pLAC, and the cut Plac constitutive expression promoter is used to reinforce the expression of small peptides Ehp. The invention also discloses the method for preparing an anti-tumor targeting engineering bacterial agent. Animal test results show that the anti-tumor targeting engineering bacteria have the quite high targeting property on tumors, but do not have obvious adverse influence on normal lab mice, and the bacterial agent prepared by the anti-tumor targeting engineering bacteria is a medicament for effectively treating tumors.

A transferring-polyethanediol-medicine molecule composition for the target therapy of tumor is disclosed. It features the combination of transferring and polyethanediol to make it has both active andpassive target functions. Its advantages are long semi-life and high accumulation degree in tumor tissue. It can be used to prepare the antineoplastic medicines.

The present invention provides a liver cancer targeting dual-group apoptotic protein expression sequence, which is a DNA sequence in which the specific AFP promoter regulates the expression of two apoptotic proteins, Caspase 3 and Granzyme B, and has the nucleoside shown in SEQID No: 1 acid sequence. The present invention utilizes the targeted regulatory effect of the AFP enhancer / promoter transcriptional regulatory element, so that the exogenous gene is only expressed in the AFP-positive liver cancer cells, so as to achieve a targeted and efficient anti-tumor effect; a recombinant activated Caspase- 3 molecule, and apply it to promote apoptosis of target cells; introduce granzyme B, and fuse granzyme B with recombinant activated Caspase-3 to mediate apoptosis of target cells. It can be used in the preparation of targeted gene medicine for treating AFP-positive liver cancer.

The invention relates to an injectable composite hydrogel loaded with a vascular disrupting agent and near-infrared optothermal response nanometer particles and applications in resisting tumor. The composite hydrogel comprises a hydrogel substrate material, as well as the vascular disrupting agent and the near-infrared optothermal response nanometer particles loaded in the hydrogel substrate material. The prepared nanometer composite hydrogel has the sol-gel converting behavior realizing syringeability and thermal hysteresis. Under the illumination of near-infrared light, the hybridized hydrogel has the excellent optothermal property, and meanwhile, the curative effects of the vascular disrupting agent are improved by enhancing the permeability of the tumor vessels. The hybridized hydrogeladopting the combined treatment of the vascular disrupting agent and the optothermal function has good safety and curative effects in resisting tumor.

Disclosed in the present invention is an ortho ester 5-fluorouracil prodrug molecule, which is N-5-fluorouracil 1-ethyl-2-carboalkoxy-(1,3)dioxolane-4-methylamide having the structure as presented by formula (I): (I) wherein n=2-32, and m=n+2. Also disclosed are a preparation method for the prodrug molecule, a nanoparticle prepared from the prodrug molecule, and an application of the prodrug molecule. The present invention has the following advantages: the nanoparticle prepared from the ortho ester 5-fluorouracil prodrug molecule has the advantages of a small molecule prodrug and also has the advantages of a nano drug delivery system (i.e., a drug carrier); the prodrug nanoparticle has good acid degradation performance and reduced tumor cell toxicity and achieve passive targeted enrichment of drugs in tumors, the drug itself is the major part of the carrier and thus a high drug loading capacity is provided, and the nanoparticle can contain other antitumor drugs and thus achieving an effect of synergistically curing tumors; the present invention has a wide application prospect in the field of tumor treatment.

The invention discloses a multi-target anti-tumor small molecule and a derivative, a preparation method, a pharmaceutical composition and application thereof. The chemical structure of the small molecule is shown as a formula I, and the derivative relates to a pharmaceutically acceptable salt of the small molecule. The small molecule and the derivative thereof have efficient tumor inhibition effects in vivo and in vitro, have low toxicity to cells and organisms, and do not generate genotoxicity; the anti-tumor activity is achieved through the multi-target effect, the HDAC inhibition activity is provided, the p53 pathway can be effectively activated, the topoisomerase activity can be effectively inhibited, and the small molecule can be used for preparing anti-tumor drugs; the preparation method is simple and easy to operate.

The invention discloses a nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen in an acid response manner as well as a preparation method and application of the nano-drug, and belongs to the field of nano-materials. The acid-responsive nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen is prepared by the following steps: (1) preparing a hollow polydopamine material; (2) preparing an acid response nano carrier material; and (3) preparing the nano-drug capable of continuously releasing hydroxyl free radicals and singlet oxygen in an acid response manner. According to the invention, the acid-responsive nano-carrier material is synthesized, and the biological enzyme is combined to the surface or the interior of the carrier material in a chemical bonding or physical adsorption manner, so that the acid-responsive nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen is obtained. The invention provides a nano-drug capable of responding to a tumor acid environment and continuously releasing free radicals, a cascade reaction can be carried out under an acidic condition to generate a large amount of hydrogen peroxide and further generate a large amount of < 1 > O < 2 > and. OH, and the nano-drug has an efficient anti-tumor effect.