33results about How to "Efficient anti-tumor effect" patented technology

The invention provides anti-tumor targeting engineering bacteria, a bacterial agent and a method for preparing the bacterial agent. The anti-tumor targeting engineering bacteria contain an anti-tumor secretory expression vector pLAC-HA, and anti-tumor targeting engineering bacteria are named Escherichia coli LMA1 through classification. The integration expression vector used for construction is pLAC, and the cut Plac constitutive expression promoter is used to reinforce the expression of small peptides Ehp. The invention also discloses the method for preparing an anti-tumor targeting engineering bacterial agent. Animal test results show that the anti-tumor targeting engineering bacteria have the quite high targeting property on tumors, but do not have obvious adverse influence on normal lab mice, and the bacterial agent prepared by the anti-tumor targeting engineering bacteria is a medicament for effectively treating tumors.

The invention belongs to the biotechnical field, and concretely relates to a tumor necrosis factor family cell apoptosis protein fusion protein, and a preparation method and a use thereof. The fusion protein is composed of annexin, a connecting peptide and a tumor necrosis factor family cell apoptosis protein, and coding gene of the fusion protein is constructed through cloning. The tumor necrosis factor family cell apoptosis protein fusion protein has a substantial enhanced cell apoptosis induction effect, can induce the apoptosis of tumor cells insensitive to the cell apoptosis, and can reduce the protein administration dosage needed by the obtaining of the treatment effect.

The invention discloses a multi-stage liver-targeted intelligent nano drug delivery system, and belongs to the field of biological materials. The nano drug delivery system comprises hydrophobic dendrimers, hydrophilic natural macromolecular polysaccharides, active targeting groups and hydrophobic drug molecules. According to the nano drug delivery system provided by the invention, the hydrophobic dendrimers are linked to the hydrophilic natural macromolecular polysaccharides by virtue of environment-responsive chemical bonds, so that amphipathic molecules are formed; then, hydrophobic drugs are encapsulated in hydrophobic cavities of nanoparticles formed by the amphipathic molecules under hydrophilic and hydrophobic actions as well as a [pi]-[pi] stacking action; and the active targeting groups, which have a tumor specific recognition function, are linked to the natural macromolecular polysaccharides by virtue of stable covalent bonds. According to the drug delivery system, intelligent nanoparticles having an environment-responsive performance can be formed through self-assembling; the drug delivery system is good in biocompatibility and biodegradability, multi-stage liver targeting can be achieved, and the drug delivery system is applicable to delivery of such hydrophobic molecules as antineoplastic drugs, fluorescent molecules, photosensitizers and the like.

The invention relates to an injectable composite hydrogel loaded with a vascular disrupting agent and near-infrared optothermal response nanometer particles and applications in resisting tumor. The composite hydrogel comprises a hydrogel substrate material, as well as the vascular disrupting agent and the near-infrared optothermal response nanometer particles loaded in the hydrogel substrate material. The prepared nanometer composite hydrogel has the sol-gel converting behavior realizing syringeability and thermal hysteresis. Under the illumination of near-infrared light, the hybridized hydrogel has the excellent optothermal property, and meanwhile, the curative effects of the vascular disrupting agent are improved by enhancing the permeability of the tumor vessels. The hybridized hydrogeladopting the combined treatment of the vascular disrupting agent and the optothermal function has good safety and curative effects in resisting tumor.

The present invention belongs to the technical fields of biomedical medicine and nano-medicine, and relates to a stem cell tumor targeting system with internal nano prodrug and a preparation method thereof. The method uses stem cells as cell carriers to prepare an anti-tumor targeting system with general formula of Stem cells-(RGD-PPCD)n through endocytosis and inner load of nano prodrug. The nano prodrug has the characteristics of targeting, sustained release and acid sensitivity release; and the stem cell carrier can remotely target primary lesion and metastasis of tumor. In vitro experimental results show that the stem cells after drug load can retain the proliferation capacity and tumor migration characteristics; and the constructed system is stable to ensure that the drug stays in the form of nano-prodrug for several days in the cells, and can slowly release the drug. In vivo experimental results show that the constructed stem cell targeting system can significantly prolong the survival time of tumor-bearing animals and can maintain normal neurobehavioral characteristics of tumor-bearing animals in comparison with the original drug and nano prodrugs, so as to play the efficient, secure and long-lasting anti-tumor effect.

The invention discloses an orthoester 5-fluorouracil prodrug molecule. The orthoester 5-fluorouracil prodrug molecule is N-5-fluorouracil1-ethyl-2-carbonalkoxy-(1,3) dioxolane-4-methylformamide havinga structure as shown in formula (I) described in the specification, wherein n=2-32 and m=n+2. The invention further discloses a preparation method of the abovementioned prodrug molecule and a nanoparticle prepared therefrom and an application thereof. The orthoester 5-fluorouracil prodrug molecule, the preparation method and the nanoparticle and the application thereof have the advantages that the nanoparticle prepared by the orthoester 5-fluorouracil prodrug molecule not only has the advantages of a small molecule prodrug but also is endowed with the advantage of a nano drug delivery system(that is a drug carrier), the prodrug nanoparticle not only has excellent acid degradation property and reduced tumor cytotoxicity and realizes passive targeting enrichment of a drug in a tumor, the nanoparticle has high drug loading capacity because the drug is used as a main part of the carrier, and the nanoparticle is capable of loading other antitumor drugs and achieves the effect of cooperatively treating the tumor and thus has good application prospects in the field of tumor treatment.

A transferring-polyethanediol-medicine molecule composition for the target therapy of tumor is disclosed. It features the combination of transferring and polyethanediol to make it has both active and passive target functions. Its advantages are long semi-life and high accumulation degree in tumor tissue. It can be used to prepare the antineoplastic medicines.

The invention discloses application of adenosine in preparing a medicament for treating tumors. In the invention, the adenosine in the field of tumor treatment is applied. In the application, injections, sustained-release preparations or targeting preparations can be prepared from the adenosine. Standard substance anti-tumor effect contrast proves that: when reaching certain concentration in vitro or in vivo, the adenosine participating in human metabolism has remarkable effects of tumor cell apoptosis and resisting tumor angiogenesis. The application has the advantages that: compared with other anti-tumor medicaments, the adenosine has efficient, broad-spectrum and low-toxicity anti-tumor effect, can be used in chemotherapy of gastric cancer, lung cancer, liver cancer, colon cancer, breast cancer and reproductive system and other malignant tumors, has remarkable anti-tumor effect, and does not have bone marrow suppression and vital organ injury by conventional dosage.

An injection of water-soluble 17-allylamino-17-demethoxy geldanamycin (17-AAG) for treating multiple kinds of cancers is prepared from 17-AAG and polyethanediol for increasing the solubility of 17-AAG in water.

The invention discloses a phospholipase A2 sensitive glycerin skeleton anti-tumor prodrug of which a molecular structure is a glycerin skeleton including a long-chain alkyl ether at a 1st position, a conjugated linoleoyl at a 2nd position and a phosphoryl nucleoside at a 3rd position. Because that a tumor tissue can highly express phospholipase A2, the glycerin skeleton anti-tumor prodrug has a tumor environmental specificity and can release a plurality of components which have activities on tumor cells at the position of the tumor. The components work in combination so that a high-efficient anti-tumor effect is achieved. An active compound of a nucleoside-type anti-tumor drug is selected from cytosine arabinoside, gemcitabine, capecitabine, fludarabine and derivatives thereof. The glycerin skeleton anti-tumor prodrug can be prepared into following high-dispersing preparations: a liposome, a nonionic surfactant vesicle, nano particles, a nano emulsion or a self-assembling transmission system.

The invention relates to the technical field of traditional Chinese medicine preparation and in particular relates to a traditional Chinese medicine preparation for treating breast cancer and a preparation method thereof. Aiming at solving the technical problems, a new option is provided for treating the breast cancer. The invention discloses the traditional Chinese medicine preparation for treating the breast cancer; the traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 3000 to 60000 parts of taxus chinensis bark, 100 to 2000 parts of radix ginseng rubra and 200 to 4000 parts of liquorice root. The invention further provides the preparation method of the traditional Chinese medicine preparation for treating the breast cancer; the preparation method comprises the following steps: step 1, preparing taxus chinensis extract; step 2, preparing radix ginseng rubra extract and liquorice root extract; step 3, preparing the preparation. According to the method of the preparation, provided by the invention, a traditional common water technology is changed and harmful substances and toxins, which easily cause chronic diseases including hypertension, hyperlipidemia and hyperglycemia and endanger livers, in water are removed, so that the content and the medicinal effect of various medicine effective components are improved. The preparation provided by the invention is prepared from various natural medicines and has essential herbal medicines, so that the clinical dosage is greatly reduced; heavy burdens and untoward effects, on stomachers, the livers, kidneys, intestines and the like, are avoided, and the medication safety and the medication effectiveness are greatly improved.

The invention relates to the technical field of medicines, in particular to a medicine composition, and a preparation method and application thereof. The medicine composition provided by the invention comprises at least two of an immune checkpoint inhibitor, a medicine for degrading the extracellular matrix of a tumor tissue and a small-molecule inhibitor, wherein the immune checkpoint inhibitor and the medicine for degrading the extracellular matrix of the tumor tissue carry related genes through a carrier and can be specifically expressed and released in a tumor microenvironment; and the purpose of treating cancers can be realized by combination with the small-molecule inhibitor. Researches show that a tumor inhibition effect can be improved by the combination of the immune checkpoint inhibitor, the medicine for degrading the extracellular matrix of the tumor tissue and the small-molecule inhibitor and is better than the tumor inhibition effect obtained by independently utilizing the above three components or utilizing any two of the above three components.

The invention provides a medicine or a composition for resisting colorectal cancer. It is first discovered by the invention that in the colorectal cancer generation and development process, overexpression of specific sequence small molecule RNA (such as ath-miR158a or variants thereof) can obviously inhibit proliferation of colorectal cancer cells and promote apoptosis of the colorectal cancer cells, so that an efficient anti-tumor effect is achieved, and the application has important significance in treatment of colorectal cancer tumors.

The invention discloses a synthesis method and application of an miRNA-carrying VB12 binding type nano-composite. The synthesis method comprises the following steps: mixing and dissolving a PLGA-PEG nano-composite, vitamin B12, EDC and DMAP in an anhydrous solvent A, and performing stirring for reaction at 15-40 DEG C; and after the reaction is finished, performing dialyzing, and removing the solvent to obtain the vitamin B12 combined nano-composite. The synthesis method has the advantages of mild preparation conditions, cheap required raw materials, convenient operation, simple process, highefficiency and practicability. The prepared nano-composite optimizes nucleic acid drugs from three aspects of water solubility, stability and tumor targeting, endows the nucleic acid drugs with efficient and low-toxicity anti-tumor effects, and has a wide application prospect in the field of tumor treatment.

The invention discloses a multi-target anti-tumor small molecule and a derivative, a preparation method, a pharmaceutical composition and application thereof. The chemical structure of the small molecule is shown as a formula I, and the derivative relates to a pharmaceutically acceptable salt of the small molecule. The small molecule and the derivative thereof have efficient tumor inhibition effects in vivo and in vitro, have low toxicity to cells and organisms, and do not generate genotoxicity; the anti-tumor activity is achieved through the multi-target effect, the HDAC inhibition activity is provided, the p53 pathway can be effectively activated, the topoisomerase activity can be effectively inhibited, and the small molecule can be used for preparing anti-tumor drugs; the preparation method is simple and easy to operate.

The invention discloses a nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen in an acid response manner as well as a preparation method and application of the nano-drug, and belongs to the field of nano-materials. The acid-responsive nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen is prepared by the following steps: (1) preparing a hollow polydopamine material; (2) preparing an acid response nano carrier material; and (3) preparing the nano-drug capable of continuously releasing hydroxyl free radicals and singlet oxygen in an acid response manner. According to the invention, the acid-responsive nano-carrier material is synthesized, and the biological enzyme is combined to the surface or the interior of the carrier material in a chemical bonding or physical adsorption manner, so that the acid-responsive nano-drug capable of continuously releasing hydroxyl radicals and singlet oxygen is obtained. The invention provides a nano-drug capable of responding to a tumor acid environment and continuously releasing free radicals, a cascade reaction can be carried out under an acidic condition to generate a large amount of hydrogen peroxide and further generate a large amount of < 1 > O < 2 > and. OH, and the nano-drug has an efficient anti-tumor effect.