Graphene oxide drug carrier and its preparation method and application

A graphene and drug technology, applied in the field of graphene oxide drug carrier and its preparation, can solve problems such as lack of reaction ability, achieve high carrying ability, improve water solubility, and safe anti-tumor effect.

Active Publication Date: 2021-02-19
南方医科大学口腔医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned PTX@GO-PEG nanoparticles lack the ability to respond to external pH stimuli. How to optimize the nano-drug and customize drug release to form a precise treatment is another problem at present.

Method used

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  • Graphene oxide drug carrier and its preparation method and application
  • Graphene oxide drug carrier and its preparation method and application
  • Graphene oxide drug carrier and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This embodiment provides a graphene oxide drug carrier GO-PEG-OSA, the preparation method of which comprises the following steps:

[0051] (1) Preparation of graphene oxide dispersion

[0052] Weigh a certain amount of micron-sized graphene oxide, disperse it into distilled water to prepare a suspension with a mass concentration of 10 mg / ml, and then use an ultrasonic disperser to intermittently ultrasonicate at 4°C for 24 hours with an ultrasonic power of 600w; then centrifuge at 5000rpm for 10min, The supernatant was collected and then freeze-dried to obtain nano-graphene oxide with a particle size of about 100 nm.

[0053] Weigh a certain amount of nano-graphene oxide, disperse it with distilled water, and prepare a nano-graphene oxide dispersion liquid with a mass concentration of 5 mg / ml. Then add 1 mol / L sodium hydroxide solution, adjust the pH of the dispersion to 10, stir magnetically at room temperature for 1 hour, then centrifuge at 6000 rpm, and discard the ...

Embodiment 2

[0067] The present embodiment provides a kind of nano medicine, and its preparation method is as follows:

[0068] Take a certain amount of GO-PEG-OSA of Example 1 and disperse it in distilled water to prepare a GO-PEG-OSA dispersion with a concentration of 5 mg / L. Take 2ml of GO-PEG-OSA dispersion, add 200μL of PTX solution, stir the mixture at 4°C for 12h, and then centrifuge at 10000rpm for 20min. Subsequently, the supernatant was collected and the content of free PTX was measured by high performance liquid chromatography (HPLC). The precipitate was dispersed again with distilled water and freeze-dried to obtain the PTX-loaded nanomedicine PTX@GO-PEG-OSA.

[0069] As a comparison, when GO-PEG-OSA is replaced with GO-PEG prepared in step (2) of Example 1 during the preparation of nanomedicine, another nanomedicine PTX@GO-PEG can be obtained.

[0070] performance testing

[0071] (1) Sustained release effect

[0072] According to different release environments, the sample...

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Abstract

The invention provides a graphene oxide drug carrier and its preparation method and application. The preparation method of the graphene oxide drug carrier is as follows: (1) adding polyethylene glycol diamine to the dispersion liquid of graphene oxide, and adding polyethylene glycol diamine in the activator Under the action of the amide condensation reaction to obtain GO-PEG; (2) GO-PEG is dissolved in a solvent to configure a GO-PEG dispersion, add oxidized sodium alginate, and perform a Schiff base reaction under the action of an activator; the reaction ends After purification and drying, the graphene oxide drug carrier GO‑PEG‑OSA was obtained. Disperse GO-PEG-OSA in a solvent to form a GO-PEG-OSA dispersion, add the drug solution, stir for 10-20 hours, and centrifuge to obtain nano-medicine. The drug carrier and nanomedicine of the present invention are sensitive to pH and light and heat, have "pH / warm" dual intelligent response release characteristics, have excellent biocompatibility, stability and water solubility, and can efficiently and quickly carry drugs into lesions.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a graphene oxide drug carrier and its preparation method and application. Background technique [0002] Traditional treatment options for malignant tumors mainly include surgical resection, chemotherapy, and radiation therapy. Most of the patients are locally advanced at the time of diagnosis, and chemotherapy is one of the inevitable main methods. In addition to the side effects caused by chemotherapy on the normal tissues of patients, tumor drug resistance is a major clinical problem that cannot be ignored. Once a patient develops chemoresistance, the only option is to replace the chemotherapeutic drug or increase the dose, which will undoubtedly aggravate the side effects of the patient, reduce the compliance of treatment, and the curative effect is still uncertain. In order to overcome the drug resistance of tumors, improve the therapeutic effect of tumors, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K47/18A61K47/04A61K41/00A61K31/337A61P35/00C08G83/00C08G81/00
CPCA61K31/337A61K41/0052A61K41/0057A61K47/02A61K47/18A61K47/36A61P35/00C08G81/00C08G83/001A61K2300/00
Inventor 冯晓黎郭伟洪陈志安陈祈月张雅晴
Owner 南方医科大学口腔医院
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