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Methods for sampling and detecting microorganisms on contact surface

A microbial sampling and contact surface technology, applied in the field of microbial detection, can solve the problems of low accuracy of detection results, low efficiency of surface microorganisms, small size of cotton swabs, etc., and achieve the effects of small mechanical damage, easy operation, and improved sampling efficiency

Inactive Publication Date: 2015-11-25
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The smear method refers to the method of using cotton swabs and other swab-like collection tools to smear left and right, up and down, and up and down within the defined range of the specification plate. However, in terms of the actual sampling status of surface microorganisms, the cotton swabs are small in size, and the operation is time-consuming and laborious when sampling large-area samples. , and the sampling operation process is difficult to standardize, resulting in poor precision
The sticker method is a method of fully pasting a certain area of ​​filter paper on the surface to be tested and staying for a certain period of time to sample. This method is easier to operate than the smear method, but the efficiency of absorbing surface microorganisms during the sampling process is low, and the accuracy of the test results is low.

Method used

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  • Methods for sampling and detecting microorganisms on contact surface
  • Methods for sampling and detecting microorganisms on contact surface
  • Methods for sampling and detecting microorganisms on contact surface

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Contact surface microbial sampling method in the present embodiment, the steps are as follows:

[0032] 1) Wet sterile dry filter paper (5cm×5cm) in sterile wetting solution, the wetting solution is composed of the following components in mass percentage: peptone 0.1%, surfactant 0.1%, sodium chloride 0.85% , the balance is water;

[0033] 2) Take out the wetted filter paper with sterile tweezers and spread it on the surface to be tested, and stop for 30s to sample.

[0034] Contact surface microorganism detection method, the steps are as follows:

[0035] 1) Use sterile tweezers to remove the sampled filter paper and place it in 10 mL of PBS buffer solution at pH 7.2, vortex and mix for 30 seconds, so that the microorganisms on the filter paper are fully released and eluted into the buffer solution, and the treatment solution is obtained for later use. ;

[0036] 2) Take a small amount of treatment solution and carry out 10-fold dilution, culture and count on TSA-0....

Embodiment 2

[0040] Contact surface microbial sampling method in the present embodiment, the steps are as follows:

[0041] 1) Wet sterile dry filter paper (5cm×5cm) in sterile wetting solution, the wetting solution is composed of the following components in mass percentage: peptone 0.05%, surfactant 0.05%, sodium chloride 0.9% , the balance is water;

[0042] 2) Take out the wetted filter paper with sterile tweezers and spread it on the surface to be tested, and stop for 10s to sample.

[0043] Contact surface microorganism detection method is the same as embodiment 1.

Embodiment 3

[0045] Contact surface microbial sampling method in the present embodiment, the steps are as follows:

[0046] 1) Wet sterile dry filter paper (5cm×5cm) in sterile wetting solution, the wetting solution is composed of the following components in mass percentage: peptone 0.15%, surfactant 0.15%, sodium chloride 0.8% , the balance is water;

[0047] 2) Take out the wetted filter paper with sterile tweezers and spread it on the surface to be tested, and stop for 60s to sample.

[0048] Contact surface microorganism detection method is the same as embodiment 1.

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Abstract

The invention discloses methods for sampling and detecting microorganisms on a contact surface and belongs to the technical field of microbiological detection. The sampling method comprises the following steps: 1), sterile filter paper is placed in a sterile wetting liquid containing peptone, a surfactant and sodium chloride for wetting; 2), a to-be-detected surface is covered with the wetted filter paper, and the filter paper is retained for sampling. The detection method further comprises the following steps: the filter paper after sampling is arranged in a phosphate buffer, uniform mixing, sampling and counting are performed, and the quantity of microorganisms on unit detected surface is obtained through calculation. The sampling method is simple to operate and high in sampling efficiency, causes little mechanical damage to the microorganisms on the contact surface, is particularly suitable for batch sampling of the microorganisms on table board type contact surfaces and can improve the sampling efficiency substantially. Meanwhile, the detection precision and accuracy of the detection method are high, and the detection efficiency can be improved substantially.

Description

technical field [0001] The invention relates to a method for sampling microorganisms on a contact surface, and also relates to a method for detecting microorganisms on a contact surface, in particular to the sampling and detection of microorganisms on a countertop contact surface, and belongs to the technical field of microorganism detection. Background technique [0002] In the food processing environment, there are various microorganisms attached to the surface of food, among which food-borne pathogenic bacteria are important biological hazards that cause public health and safety problems. The contact between people and food, food and food, food and equipment during food processing will cause these microorganisms to cross-infect each other, and the infection of food by pathogenic bacteria, especially food that has been sterilized, will cause food quality problems , a threat to public health. Therefore, the detection of microorganisms on food contact surfaces in food proce...

Claims

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Application Information

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IPC IPC(8): C12Q1/24C12Q1/06
Inventor 索标艾志录李玺汪月霞王娜范会平
Owner HENAN AGRICULTURAL UNIVERSITY
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