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Efficient propagation method for African daisy virus-free seedlings

A technology of virus-free seedlings and gerbera, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low seedling survival rate, low induction rate, and low seedling multiplication efficiency, etc. The survival rate is improved, the disinfection method is simple, and the disinfection effect is good.

Inactive Publication Date: 2015-12-02
郝毅 +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of low induction rate, poor quality of induced seedlings and many weak seedlings, low seedling multiplication efficiency, and low survival rate of transplanted seedlings existing in current gerbera chrysanthemum virus-free seedling propagation methods, the present invention provides a gerbera chrysanthemum detoxification The high-efficiency multiplication method of poisonous seedlings improves the quality, proliferation efficiency and transplanting survival rate of induced seedlings by establishing and improving the culture program, culture conditions and the ratio of culture components, thus establishing a high-quality and high-quantity fast traditional system

Method used

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  • Efficient propagation method for African daisy virus-free seedlings
  • Efficient propagation method for African daisy virus-free seedlings
  • Efficient propagation method for African daisy virus-free seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0049] The high-efficiency multiplication method of the virus-free gerbera seedlings of the present embodiment was implemented in 2014-2015. The pre-treatment, plant regeneration and seedling hardening of the gerbera gerbera virus-free seedlings were carried out in Lianyungang City Zhenxing Flower Garden, and the tissue-cultured seedlings were transplanted Planted in the flower experiment base of Dongxin Farm in Lianyungang City, the detailed steps are as follows:

[0050] 1) Gerbera detoxification pre-emergence treatment:

[0051] The virus-free seedlings of the gerbera variety Sunshine Coast were used to wash them repeatedly with tap water, then washed with a brush dipped in detergent, then rinsed with ultra-pure water, soaked in 70% alcohol for 15 seconds, and then rinsed with sterile water for two times. Once, add a few drops of Tween 20 solution to 0.1% mercury liter, soak for 5 minutes, rinse with sterile water for 5 times, blot dry with filter paper, and transfer the pe...

Embodiment 2

[0068] The high-efficiency propagation method of Gerbera chrysanthemum virus-free seedlings in this embodiment, implementation time, pre-treatment of gerbera chrysanthemum virus-free seedlings, plant regeneration, seedling hardening, and tissue culture seedling transplanting are all the same as in Example 1, and the culture medium (liquid) The formulation, comparison medium (solution) formulation and operation steps are the same as in Example 1. The biggest difference between this example and Example 1 is that the Gerbera chrysanthemum virus-free seedling variety is replaced by Linglong:

[0069] 1) Gerbera detoxification pre-emergence treatment:

[0070] The virus-free seedlings of the gerbera variety Linglong are used to wash them repeatedly with tap water, wash them once with a brush dipped in detergent, then rinse them with ultra-pure water, soak them in 70% alcohol for 15 seconds, and rinse them twice with sterile water , add a few drops of Tween 20 solution to 0.1% mercu...

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Abstract

The invention discloses an efficient propagation method for African daisy virus-free seedlings, which comprises the following steps: (1) carrying out pretreatment on African daisy virus-free seedlings; (2) inducing adventitious buds; (3) cutting the adventitious buds, and carrying out subculture multiplication; (4) hardening the seedlings; (5) carrying out water culture for rooting; (6) carrying out water culture for reinforcing the seedlings; and (7) transplanting. The efficient propagation method disclosed by the invention solves the problems of low efficiency and easy secondary pollution of the African daisy virus-free seedlings in a propagation process, is capable of propagating a lot of good African daisy virus-free seedlings in a short time and also avoiding the problem of variety degeneration caused by African daisy division propagation and seed propagation, is simple to operate and easy to realize industrialized popularization, and has relatively high real values in production.

Description

technical field [0001] The invention relates to a method for efficiently multiplying virus-free gerbera seedlings, in particular to a method for multiplying a large number of good gerbera-free seedlings in a short period of time, and belongs to the technical field of flower planting. Background technique [0002] Gerbera ( Gerbera jamesonii Bolus), also known as gerbera and sunflower, is a perennial herb of the Compositae family. It is native to the Devasland region of South Africa, and then introduced to the UK. Now it is widely cultivated all over the world. Gerbera likes warm winters and cool summers. The flowers are large, delicate and upright, elegant and elegant, colorful but not coquettish, as beautiful as clouds, charming and elegant, refreshing and pleasing to the eye, giving people a sense of chic, soft, tranquil and serene. Gerbera is often used to cut flowers and place in vases. If it is paired with green foliage plants such as Kidney Tibetan and Asparagus, it w...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 郝毅
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