Targeting-light double-therapy carbon nanometer complex, preparation method and applications thereof

A technology of carbon nano-composites, which is applied in the field of biomedical materials, can solve the problems of no active targeting, and achieve the effect of enhancing killing efficiency and simple preparation method

Inactive Publication Date: 2015-12-02
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many dual phototherapy systems based on the combination of photothermal therapy and photodynamic therapy based on carbon nanohorns, but there is no active targeting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0029] Preparation of carbon nanohorn / chlorin e6-hyaluronic acid complex

[0030] Follow the steps below:

[0031] (1) 200 mg of hyaluronic acid was dissolved in 10 mL of phosphate buffer (PBS, pH 7.4), and then activated by adding EDC (50.4 mg) and NHS (30.4 mg). After 15 minutes, add 153 μL of PEG with amino groups at both ends to the activated reaction solution. The reaction was stirred for 12 hours (overnight). The reaction solution was transferred into a dialysis bag for dialysis for three days, and then freeze-dried to obtain PEGylated hyaluronic acid.

[0032] (2) 15 mg of chlorin e6 was dissolved in 7.5 mL of DMSO, and activated by adding EDC (24 mg) and NHS (14.4 mg) for 1 hour. The PEGylated hyaluronic acid obtained in the 28.3mg step (1) was dissolved in DMF:H 2 O mixed solvent (1:1v / v, 3mL) was added to the activated chlorin e6 solution, and the reaction was stirred for 12 hours (overnight). The reaction solution was dialyzed in a dialysis bag for three days a...

preparation Embodiment 2

[0036] Preparation of monolayer uncurled graphene / chlorin e6-hyaluronic acid complex

[0037] According to Example 1, the difference is that in step (3), the single-walled carbon nanohorn is replaced by a single-layer uncurled graphene.

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Abstract

The present invention relates to a targeting-light double-therapy carbon nanometer complex, which is a [pi]-[pi] conjugated complex formed from a conjugate formed from water-soluble chlorin e6-hyaluronic acid and single-walled carbon nano-horns or single-layer non-curling graphene, wherein the mass fraction of the chlorin e6 is 1-35.00%, and the mass fraction of the single-walled carbon nano-horns or single-layer non-curling graphene is 30-60%. The complex has multiple therapy effects of photo-thermal therapy, photodynamic therapy, targeting therapy, and cancer cell killing efficiency enhancing, further has enzyme response, and can release the photodynamic reagent chlorin e6 under the effect of hyaluronidase.

Description

【Technical field】 [0001] The invention relates to the technical field of biomedical materials, in particular to a phototherapeutic agent, and more specifically to a carbon nanocomposite for targeted photo-double therapy and its preparation method and application. 【Background technique】 [0002] Phototherapy is a non-invasive treatment technology using visible light or near-infrared light irradiation. It has unique advantages such as remote controllability, high selectivity and low systemic toxicity. It has broad application prospects in the treatment of malignant tumors. Photothermal therapy and photodynamic therapy are two different forms of phototherapy currently widely used in tumor treatment. Photothermal therapy is the use of photothermal reagents to absorb light of specific wavelengths, and convert the absorbed energy into heat through non-radiative attenuation, resulting in an increase in local environmental temperature and irreversible damage to diseased cells. Phot...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/48A61P35/00
Inventor沈星灿蒋邦平胡兰芳董配施祖进
OwnerGUANGXI NORMAL UNIV