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Low-concentration NAA gel root dipping method

A low-concentration, gel technology, applied in the field of plant biology, can solve problems such as high cost, loss of branches, instability, etc., and achieve the effect of easy operation and reduced water evaporation

Inactive Publication Date: 2015-12-09
钱夕华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for dipping roots with low-concentration NAA gel, characterized in that the method includes hormone mother liquor preparation, working solution preparation, hormone gel preparation, root dipping, and cuttings, and the specific steps are as follows:

[0014] ① Hormone mother solution preparation: Accurately weigh 0.1g of NAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL NAA mother solution;

[0015] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:100 into a low-concentration hormone working solution with a concentration of 10mg / L;

[0016] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethyl cellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone working solution with a concentration of 10 mg / L in a water bath at 50 °C for later use;

[0017] Root dipping: Dip the lower end of the 8-10cm long, healthy and strong bo...

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PUM

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Abstract

The invention discloses a low-concentration NAA gel root dipping method, and belongs to the field of plant biotechnology. The root dipping method comprises the steps of: first, compounding an NAA mother liquid of 1mg / mL; then diluting the mother liquid to a low-concentration NAA working liquid of 10mg / L; using the working liquid to compound a low-concentration NAA gel sealing liquid, wherein the mass concentration of carboxymethyl cellulose sodium is 1%, and the mass concentration of sodium alginate is 8%; dipping lower ends of healthy and strong branches in the gel sealing liquid for 3-5 seconds, and taking out the dipped branches; and inserting the dipped branches into a cultivate substrate for rooting.

Description

technical field [0001] The invention discloses a root dipping method of low-concentration NAA gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as NAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagation or ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/40A01P21/00A01G7/06A01G1/00
Inventor 钱夕华
Owner 钱夕华