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Compounding method of medium-concentration KT and glycerine gel

A preparation method, glycerin gel technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, unsatisfactory production effect, etc., and achieve the effect of reducing water evaporation and easy operation

Inactive Publication Date: 2015-12-09
何其家
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0011] A preparation method of medium concentration KT and glycerin gel, characterized in that the method includes the preparation of hormone mother solution, the preparation of working solution, and the preparation of hormone gel, and the specific operation steps are as follows:

[0012] ① Hormone mother liquor preparation: Accurately weigh 0.1g of KT and dissolve it with a little 1M KOH, then dilute to 100mL with distilled water, which is 1mg / mL KT mother liquor;

[0013] ② Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:10 into a medium-concentration hormone working solution with a concentration of 100mg / L, and add 10ml of glycerin to 90ml of hormone working solution;

[0014] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone and glycerol working solution with a concentration of 100 mg / L in a water bath at 50°C for later use.

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Abstract

The invention discloses a compounding method of medium-concentration KT and glycerine gel, and belongs to the field of plant biotechnology. The compounding method comprises the steps of: firstly compounding a KT mother liquid of 1mg / mL; diluting the mother liquid to a medium-concentration KT and glycerine working liquid of 100mg / L; and using the working liquid to compound a medium-concentration KT and glycerine gel sealing liquid, wherein the mass concentration of carboxymethyl cellulose sodium is 1%, and the mass concentration of sodium alginate is 8%.

Description

technical field [0001] The invention discloses a method for preparing medium-concentration KT and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro prop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/90A01P21/00
Inventor 何其家
Owner 何其家