Simulated soil solution test apparatus and method for evaluating toxicity of pollutants to earthworms

Abstract

The invention relates to a simulated soil solution test apparatus and method for evaluating toxicity of pollutants to earthworms. The apparatus includes light transmitting and watertight incubators, each incubator is internally provided with a monitoring device, and the incubators are connected to an oxygen supply device. The method consists of: preparing a simulated solution containing the main components of a soil solution; adding environmental pollutants of different concentrations into the simulated solution, and conducting numbering respectively, and also taking a simulated solution without adding of environmental pollutants as the experimental control; adding the environmental pollutants added simulated solutions with different numbers into different incubators, putting intestine cleared earthworms into the incubators, and exposing the earthworms in the solutions completely; and introducing oxygen into the cubators through an oxygen supply device, and observing and recording the behavior reaction and fatality rate of the earthworms in different environmental pollutants' simulated solutions within a certain period of time through cameras. The test apparatus provided by the invention has the advantages of simplicity, good test reproducibility and high monitoring sensitivity, etc., thus having broad application prospects in the field of contaminated soil ecological risk assessment.

Description

technical field [0001] The invention relates to a simulated soil solution test device and method for evaluating the toxicity of pollutants to earthworms. Background technique [0002] In traditional soil pollution experiments, due to the complex soil composition and significant differences in soil background, the toxicity of pollutants is greatly disturbed, and the results shown by different soils are very different, resulting in complicated experimental equipment and poor experimental reproducibility. The way earthworms absorb pollutants in the soil is mainly through their skin passively absorbing the active ingredients dissolved in the simulated soil solution. Therefore, it is of great significance to develop a soil pollution experiment that takes earthworms as the experimental object, has a short experimental period, good reproducibility and high sensitivity. Contents of the invention [0003] The object of the present invention is to provide a kind of simulated soil s...

AI Technical Summary

Problems solved by technology

[0002] In traditional soil pollution experiments, due to the complex soil composition and significant differences in soil background, the toxicity of pollutants is greatly interfered, and the results shown by different soils are very different, resulting in complicated experimental equipment and poor experimental reproducibility.

Images