Microfluidic chip for rapid detection of ELISA and its pretreatment and detection method

Abstract

The invention discloses an enzyme-linked immune rapid detection microfluidic chip and a pretreatment and detection method thereof. The microfluidic chip includes a first chip layer and a second chip layer; at least one detection channel is distributed in the first chip layer and is provided with a first sample inlet, a second sample inlet and a sample outlet, and the second sample inlet is located between the sample outlet and the first sample inlet; the second chip layer can move relative to the first chip layer and is provided with a third sample inlet and a fourth sample inlet, and conditions are met: when the second chip layer is at a first position, the third sample inlet communicates with the first sample inlet, and the fourth sample inlet does not communicate with the second sample inlet; and when the second chip layer is at a second position, the third sample inlet does not communicate with the first sample inlet, and the fourth sample inlet communicates with the second sample inlet. The amount of detection used samples is greatly reduced; after pretreatment, after a to-be-detected sample is incubated, a detection reagent is added and a result can be quickly analyzed.

Description

technical field [0001] The application belongs to the technical field of enzyme-linked immunoassay rapid detection, and in particular relates to a microfluidic chip for enzyme-linked immunoassay rapid detection and its pretreatment and detection methods. Background technique [0002] The traditional enzyme-linked immunoassay (ELISA) is operated on a 96-well plate. First, the primary antibody is added to the well plate for incubation. After a certain period of time, the antibody is adsorbed on the surface of the well plate. After washing away the unadsorbed antibody, block the solid surface that does not absorb the primary antibody to reduce non-specific adsorption of proteins. Then, add a standard substance containing a known antigen or a sample to be tested. After incubation for a certain period of time, add a specific enzyme-labeled secondary antibody. After incubation for a certain period of time, the secondary antibody binds to the antigen, and then washes to remove the ...

AI Technical Summary

Problems solved by technology

At present, there is a great demand for ELISA consumables and automatic ELISA in the market. The existing ELISA plate is not only expensive, but also unable to detect the content of multiple target proteins at the same time.

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