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Preparation method of low concentration NAA and glycerin gel

A technology of glycerin gel and preparation method, which is applied in the field of plant biology, can solve the problems of high cost, loss of branches, instability, etc., and achieve the effect of easy operation and reduced water evaporation

Inactive Publication Date: 2015-12-23
钱夕华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0011] A method for preparing low-concentration NAA and glycerin gel, characterized in that, the method includes hormone mother solution preparation, working solution preparation, and hormone gel preparation, and the specific operation steps are as follows:

[0012] ① Hormone mother solution preparation: Accurately weigh 0.1g of NAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL NAA mother solution;

[0013] ②Preparation of working solution: Dilute the above mother solution into a low-concentration hormone working solution with a concentration of 10mg / L according to the volume ratio of 1:100, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0014] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone and glycerol working solution with a concentration of 10 mg / L in a water bath at 50°C for later use.

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Abstract

The invention discloses a preparation method of low concentration NAA and glycerin gel, and belongs to the technical field of plant biology. The preparation method comprises the following steps: at first, preparing a NAA mother liquor with a concentration of 1 mg / mL, diluting the mother liquor into a low concentration NAA and glycerin work fluid with a concentration of 10 mg / L; using the work fluid to prepare a low concentration NAA and glycerin gel confining liquid, which contains sodium carboxymethyl cellulose with a mass concentration of 1% and sodium alginate with a mass concentration of 8%.

Description

technical field [0001] The invention discloses a method for preparing low-concentration NAA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as NAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N37/10A01P21/00A01G1/00A01G7/06
Inventor 钱夕华
Owner 钱夕华