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Method for measuring polycyclic aromatic hydrocarbons in cigarette cut tobacco by on-line solid phase extraction and high performance liquid chromatography

A technology of high performance liquid chromatography and polycyclic aromatic hydrocarbons, applied in the field of physical and chemical inspection of cigarette shreds

Inactive Publication Date: 2015-12-23
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this method, after the sample is extracted by solvent, it needs to be purified and concentrated by solid-phase extraction column before GC / MS analysis, which consumes more solvents and is easy to cause environmental pollution; the mixed solvent of ethanol and ether established by Zhang Junsong et al. Extraction, purification by neutral alumina column chromatography, and high performance liquid chromatography are also relatively cumbersome methods

Method used

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  • Method for measuring polycyclic aromatic hydrocarbons in cigarette cut tobacco by on-line solid phase extraction and high performance liquid chromatography
  • Method for measuring polycyclic aromatic hydrocarbons in cigarette cut tobacco by on-line solid phase extraction and high performance liquid chromatography
  • Method for measuring polycyclic aromatic hydrocarbons in cigarette cut tobacco by on-line solid phase extraction and high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036] 1. Reagents and instruments:

[0037] Standard: 11 mixed standard samples of polycyclic aromatic hydrocarbons (all 11 polycyclic aromatic hydrocarbons have a concentration of 2.0mg / ml), dissolved in dichloromethane, including: naphthalene, fluorene, phenanthrene, anthracene, fluoranthene, benzo(a ) anthracene, benzo(b) fluoranthene, benzo(k) fluoranthene, benzo(a) pyrene, dibenzo(a, h) anthracene, benzo(g, h, i) perylene, Ampex ); 1-methylnaphthalene and 2-methylnaphthalene are all purchased from AccuStandard company;

[0038] Reagents: acetonitrile (chromatographically pure, Duksanpurechemicals, Korea), cyclohexane (chromatographically pure, JTBaker, USA). The experimental water was deionized water produced by Milli-Q pure water system.

[0039] Instrument: ThermoUltiMate3000 high performance liquid chromatograph, equipped with six-channel online degasser, double ternary gradient pump, autosampler (with large volume sampling components), column thermostat (with a 2p...

example 2

[0050] The specific steps are as described in Example 1. Another sample B was selected, and the content of polycyclic aromatic hydrocarbons in shredded cigarette tobacco was measured in Table 6.

[0051] compound Content (ng / g) naphthalene 20.6 1-Methylnaphthalene 6.9 2-Methylnaphthalene 5.0 Fluorene 7.4 Philippines 10.6 Anthracene 53.2 Fluoranthene N.D. Benz(a)anthracene N.D. Benzo(b)fluoranthene N.D. Benzo(k)fluoranthene N.D. Benzo(a)pyrene N.D. Dibenzo(a,h)anthracene N.D. Benzo(g,h,i)perylene 16.9

[0052] Note: N.D. means not detected.

example 3

[0054] The specific steps are as described in Example 1. Another sample C was selected, and the content of polycyclic aromatic hydrocarbons in shredded cigarette tobacco was measured in Table 7.

[0055] compound Content (ng / g) naphthalene 20.9 1-Methylnaphthalene 6.5 2-Methylnaphthalene 5.2 Fluorene 6.7 Philippines 10.3 Anthracene 49.8 Fluoranthene N.D. Benz(a)anthracene N.D. Benzo(b)fluoranthene N.D. Benzo(k)fluoranthene N.D. Benzo(a)pyrene N.D. Dibenzo(a,h)anthracene N.D. Benzo(g,h,i)perylene 6.3

[0056] Note: N.D. means not detected.

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Abstract

The invention discloses a method for measuring polycyclic aromatic hydrocarbons in cigarette cut tobacco by on-line solid phase extraction and high performance liquid chromatography. The polycyclic aromatic hydrocarbons refer to the following thirteen compounds: naphthalene, 1-methylnaphthalene, 2-methylnaphthalene, fluorene, phenanthrene, anthracene, fluoranthene, benzo (a) anthracene, benzo (b) fluoranthene, benzo (k) fluoranthene, benzo (a) pyrene, dibenzo (a,h) anthracene and benzo (g,h,i) perylene. The method is characterized in that the polycyclic aromatic hydrocarbons in the cigarette cut tobacco are extracted by adopting a triangular flask holding cyclohexane, and the content of the polycyclic aromatic hydrocarbons in the cigarette cut tobacco is measured by utilizing the on-line solid phase extraction and the high performance liquid chromatography. The method has the advantages that the content of the polycyclic aromatic hydrocarbons in the cigarette cut tobacco can be rapidly and efficiently detected; pretreatment is simple; average relative standard deviation is less than 5 percent, and the average recovery rate of all indexes is between 83.6 percent to 104.1 percent; the method has the characteristics of being rapid, accurate, high in sensitivity and high in repeatability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical inspection of shredded cigarette tobacco, in particular to an online solid-phase extraction high-performance liquid chromatography method for determining polycyclic aromatic hydrocarbons in shredded cigarette tobacco. Background technique [0002] PAHs have been widely concerned in the research of cigarette smoke. At the same time, PAHs in cigarette shreds will also transfer to mainstream smoke during the burning process of cigarettes, thereby affecting the content of PAHs in mainstream smoke. PAHs are considered by the International Agency for Research on Cancer (IARC) as human carcinogens (category 1) or suspected human carcinogens (category 2B), so it is necessary to analyze and study PAHs in shredded cigarettes. Benzo[a]pyrene (BaP) is the most carcinogenic component in polycyclic aromatic hydrocarbons (PAHs), and accurate determination of BaP is of great significance for the haz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/14
Inventor 张洪非李雪罗彦波姜兴益朱风鹏刘洋庞永强侯宏卫
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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