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A method for detecting species mixing in the cooling water process of kelp summer seedlings

A technology of kelp and varieties, applied in the fields of botanical equipment and methods, horticulture, seaweed cultivation, etc., can solve problems such as mixed varieties

Active Publication Date: 2017-12-05
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, if more than two varieties are cultivated simultaneously in the shared circulating water system, it may cause the problem of mixed varieties.
In the actual production of kelp seedlings, there has been no effective technical method to detect whether there is variety mixing.

Method used

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  • A method for detecting species mixing in the cooling water process of kelp summer seedlings
  • A method for detecting species mixing in the cooling water process of kelp summer seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Long-term preservation of vegetatively grown filamentous kelp monoclonal female gametophytes (for the acquisition and growth of female gametophytes, please refer to Li Jing, Pang Shaojun, Shan Tifeng, Liu Feng, Gao Suqin. Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae). Chinese Journal of Oceanology and Limnology.2014.32 (2): 365-371. Records in the literature), placed in a high-speed crushing machine, the filamentous female gametophytes were broken to 3-10 cells at a speed of 2000r / min. Subsequently, the crushed female gametes were aerated with 50 cm seedling ropes and cultured in a 1L beaker, placed in PES medium, at a temperature of 12°C, a light intensity of 10-20 μmolphotons m-2 s-1, and a photoperiod of 12 :12(L:D) light incubator.

[0022] When the female gametophyte is firmly attached to the seedling rope, increase the culture light to 50-60 μmol photons m-2s-1, so that the female gametophyte develops and forms oocysts. Subsequent...

Embodiment 2

[0028] In August 2014, a kelp nursery enterprise in Rongcheng City, Shandong Province, my country carried out the detection of species mixing in the refrigeration water circulation system. The seedling ropes inoculated with female gametophyte clones were placed in the seedling ponds and water inlets of the low-temperature circulating water system, and the seedling ropes were collected later. The sporophytes of the progeny above were compared with the parthenogenetic sporophytes as a control. At the same time, using the microsatellite analysis method, four pairs of SSR primers were used to amplify, and the parental female gametophyte was used as a reference to analyze the genotype of the offspring sporophyte. It showed that the sporophytes of the offspring collected at the nursery pool and the water tank were normal in shape. In addition to the gene fragments of the parental female gametophytes, exogenous gene fragments were also detected in this sporophyte group, which proved to...

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Abstract

The invention relates to the field of economical brown kelp seaweed varietal complexity, in particular to a method for detecting varietal complexity in kelp summer seedling refrigeration water process. The method comprises the steps that a seedling rope which is inoculated with a female gametophyte clone is put in a culture medium or a kelp seedling refrigeration water circulation system to be cultured, parthenogernesis sporophore is obtained when the seedling rope is put in the culture medium, and offspring sporophore is obtained when the seedling rope is put in the kelp seedling refrigeration water circulation system; morphological characteristics and genetype of the offspring sporophore on the seedling rope in the refrigeration water circulation system are detected and compared with those of the parthenogernesis sporophore so as to determine whether varieties are hybrid or not in the seedling process. According to the detection method, it is verified for the first time that in the kelp summer seedling production process, due to the fact that low temperature circulation water is used in common, the varietal complexity is definitely caused.

Description

technical field [0001] The invention relates to the field of mixed species of economic brown algae kelp, in particular to a method for detecting mixed varieties of kelp summer seedlings in a cooling water process. Background technique [0002] Kelp is a typical cold-water seaweed. In my country's sea waters, due to the high sea temperature in summer, it cannot complete the life history process in natural sea waters. Since the 1950s, the breakthrough in the cultivation technology of kelp summer seedlings in my country has promoted the rapid expansion of kelp cultivation scale. The summer seedling technology is to artificially cool down the high-temperature seawater in summer, and maintain the seawater temperature at 5-12°C, which is suitable for the survival of kelp, so as to complete the process of cultivating kelp seedlings indoors. In order to save cooling costs, kelp seedling enterprises recycle cooling water in production. Therefore, if more than two species are cultiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
CPCA01G33/00Y02A40/80
Inventor 李静逄少军单体锋
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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