Preparation method for inactivated vaccine against avian pasteurellosis

A technology of Pasteurella fowl and inactivated vaccine, which is applied in the field of pathogenic microbiology, can solve the problems such as the limitation of immune effect of inactivated vaccine, and achieve the effect of good immune protection.

Inactive Publication Date: 2016-02-03
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is why the immune effect of conventional inacti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for inactivated vaccine against avian pasteurellosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] A preparation method of poultry pasteurellosis inactivated vaccine, the specific preparation steps are as follows:

[0026] (1) Preparation of strains for production

[0027] Inoculate Pasteurella avian standard strain type A NCTC1502 on TSA (trypticsoyagar tryptic soybean agar) solid medium plate, and cultivate and recover in a 37°C incubator;

[0028] (2) Preparation of vaccine preparation solution

[0029] Pick typical colonies and inoculate them in TSB (trypticso...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method for an inactivated vaccine against avian pasteurellosis. The preparation method comprises the following steps: preparation of a strain used for production; preparation of bacterial liquid used for vaccine preparation; inactivation of the bacterial liquid used for vaccine preparation; and preparation of the vaccine. According to the method, avian Pasteurella multocida type A is used as the original strain; the strain undergoes culture in an iron-limited environment so as to obtain the bacterial liquid eventually; and the bacterial liquid is mixed with a white oil adjuvant so as to prepare the inactivated vaccine against avian pasteurellosis. A local chicken is immunized with the vaccine prepared by using the method, then challenge test is carried out, and results show than a protection rate reaches 100%, so it is proved that the vaccine exerts good immunoprotection effect.

Description

technical field [0001] The invention relates to the field of pathogenic microbiology, in particular to a preparation method of an inactivated poultry pasteurellosis vaccine. Background technique [0002] Avian Pasteurellosis, also known as fowl cholera, is an acute, contact septicemia infectious disease caused by Pasteurella multocida (Pm). Impact. The disease is mainly transmitted through the digestive tract, respiratory tract, mucous membranes and skin wounds, and the main source of infection is invisible infected poultry and sick poultry. Bacteria-carrying poultry is the most dangerous and most difficult to prevent potential source of infection. Its appearance does not show any abnormalities, but it often excretes germs, polluting the surrounding environment, feed, drinking water and utensils, thereby infecting the surrounding healthy chickens, forming a disease. important spreading factor. The excrement of sick poultry pollutes drinking water and feed, and can be tran...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/102C12N1/20A61P31/04C12R1/01
Inventor 罗青平邵华斌张腾飞
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap