Method for measuring antioxidant activity of materials

A technology of antioxidant activity and substances, applied in the research field of medicine, food and health care products, can solve the problems of inaccurate test results, high experimental cost, troublesome operation, etc., and achieves low equipment requirements, universal applicability, and low cost. Effect

Inactive Publication Date: 2016-04-13
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the subject of the in vivo test is human or animal, the cost of the test is high, the test cycle is long, and the operation is troublesome, etc., which is not suitable fo

Method used

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  • Method for measuring antioxidant activity of materials
  • Method for measuring antioxidant activity of materials
  • Method for measuring antioxidant activity of materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The establishment of the oxidative stress injury model of Escherichia coli by hydrogen peroxide

[0034] 1. Experimental materials

[0035] Escherichia coli CMCC (B) 44102, purchased from Guangdong Huankai Microbial Technology Co., Ltd., No. 788, Shenzhou Road, Science City, Guangzhou Development Zone, Luogang District, Guangzhou;

[0036] Hydrogen peroxide (30%H 2 o 2 , analytically pure, Hongyan Reagent Factory, East District, Tianjin).

[0037] 2. Experimental method

[0038] Escherichia coli CMCC (B) 44102, inoculated in LB medium (yeast extract: 5g, peptone: 10g, NaCl: 10g, water: 1000ml), placed in a shaker for 10-11 hours overnight, the shaker speed is per minute 150 revolutions, the temperature is 37°C. Pipette a small amount of bacteria in the logarithmic growth phase under the ultra-clean bench and dilute to OD with LB medium 600 The value is about 0.2, and then the oxidative stress factor hydrogen peroxide is added. The volume of the reaction...

Embodiment 2

[0041] Example 2 The protective effect of ferulic acid on hydrogen peroxide damage Escherichia coli model

[0042] 1. Experimental materials

[0043] Escherichia coli CMCC (B) 44102, purchased from Guangdong Huankai Microbial Technology Co., Ltd., No. 788, Shenzhou Road, Science City, Guangzhou Development Zone, Luogang District, Guangzhou;

[0044] Hydrogen peroxide (30%H 2 o 2 , analytically pure, Tianjin East District Hongyan Reagent Factory);

[0045] Ferulic acid (FerulicAcid), 3-methoxy-4-hydroxycinnamic acid.

[0046] 2. Experimental method

[0047] Pipette Escherichia coli CMCC (B) 44102 in the logarithmic growth phase and dilute to OD with LB medium 600The value is about 0.3, add ferulic acid and Escherichia coli to the multi-well plate and let it stand for 30 minutes at room temperature. The ferulic acid used is prepared with ethanol solution, and the prepared ferulic acid concentration gradient is: 40 μM, 20 μM, 10 μM, 5 μM.

[0048] Hydrogen peroxide was the...

Embodiment 3

[0053] Example 3 The protective effect of cinnamic acid compounds on hydrogen peroxide damage Escherichia coli model

[0054] 1. Experimental materials

[0055] Strains:

[0056] Escherichia coli CMCC (B) 44102 (provided by Lanzhou Food and Drug Inspection Institute, Gansu Province);

[0057] Samples for testing:

[0058] (1) Caffeic acid: prepared with cold ethanol solution, the prepared caffeic acid concentration gradient is: 20 μM, 10 μM;

[0059] (2) Ferulic acid: prepared with ethanol solution, the prepared ferulic acid concentration gradient is: 20 μM, 10 μM;

[0060] (3) p-hydroxycinnamic acid: prepared with physiological saline, the prepared p-hydroxycinnamic acid concentration gradient is: 20 μM, 10 μM;

[0061] (4) Cinnamic acid: prepared with ethanol solution, the prepared cinnamic acid concentration gradient: 20 μM, 10 μM.

[0062] figure 2 It is the structural formula of each test sample, that is, the cinnamic acid compound.

[0063] 2. Experimental method...

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Abstract

The invention belongs to the field of researches of medicines, foods and healthcare products and discloses a method for measuring antioxidant activity of materials and application of the method to researches of the medicines, the foods and the healthcare products. The method is characterized in that a standard escherichia coli strain is used as an experimental model, and hydrogen peroxide is used as a radical initiator to treat escherichia coli in a logarithmic phase so as to enable the escherichia coli to be in an oxidative stress state. By extrinsic introduction of an antioxidant, a bacterial oxidation stress protection function of the antioxidant can be verified. The method has the advantages of simplicity and feasibility in operation, low equipment requirement, low cost and high application value.

Description

technical field [0001] The invention relates to a method for measuring the antioxidant activity of a substance, in particular to the application of the method for measuring the antioxidant activity of a substance in the research of medicines, food and health products thereof, and belongs to the field of research of medicines, food and health care products thereof. Background technique [0002] Excess free radicals in the body (oxidative stress, Oxidative stress) can cause oxidative damage to biomembrane lipids, proteins, and DNA, leading to cancer, aging, arteriosclerosis, and various chronic diseases of the elderly. [0003] Antioxidants can interfere with the initiation and spread of active oxygen free radical chain reactions, and finally block the free radical reaction process, thereby effectively reducing the poisoning effect of oxygen free radicals. The human body regulates the redox balance in the body by supplementing antioxidants to achieve the purpose of preventing ...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/19
Inventor 李红玉马晓燕郑丽芳包艳芳
Owner LANZHOU UNIVERSITY
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