Method for measuring antioxidant activity of materials
A technology of antioxidant activity and substances, applied in the research field of medicine, food and health care products, can solve the problems of inaccurate test results, high experimental cost, troublesome operation, etc., and achieves low equipment requirements, universal applicability, and low cost. Effect
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Embodiment 1
[0033] Example 1 The establishment of the oxidative stress injury model of Escherichia coli by hydrogen peroxide
[0034] 1. Experimental materials
[0035] Escherichia coli CMCC (B) 44102, purchased from Guangdong Huankai Microbial Technology Co., Ltd., No. 788, Shenzhou Road, Science City, Guangzhou Development Zone, Luogang District, Guangzhou;
[0036] Hydrogen peroxide (30%H 2 o 2 , analytically pure, Hongyan Reagent Factory, East District, Tianjin).
[0037] 2. Experimental method
[0038] Escherichia coli CMCC (B) 44102, inoculated in LB medium (yeast extract: 5g, peptone: 10g, NaCl: 10g, water: 1000ml), placed in a shaker for 10-11 hours overnight, the shaker speed is per minute 150 revolutions, the temperature is 37°C. Pipette a small amount of bacteria in the logarithmic growth phase under the ultra-clean bench and dilute to OD with LB medium 600 The value is about 0.2, and then the oxidative stress factor hydrogen peroxide is added. The volume of the reaction...
Embodiment 2
[0041] Example 2 The protective effect of ferulic acid on hydrogen peroxide damage Escherichia coli model
[0042] 1. Experimental materials
[0043] Escherichia coli CMCC (B) 44102, purchased from Guangdong Huankai Microbial Technology Co., Ltd., No. 788, Shenzhou Road, Science City, Guangzhou Development Zone, Luogang District, Guangzhou;
[0044] Hydrogen peroxide (30%H 2 o 2 , analytically pure, Tianjin East District Hongyan Reagent Factory);
[0045] Ferulic acid (FerulicAcid), 3-methoxy-4-hydroxycinnamic acid.
[0046] 2. Experimental method
[0047] Pipette Escherichia coli CMCC (B) 44102 in the logarithmic growth phase and dilute to OD with LB medium 600The value is about 0.3, add ferulic acid and Escherichia coli to the multi-well plate and let it stand for 30 minutes at room temperature. The ferulic acid used is prepared with ethanol solution, and the prepared ferulic acid concentration gradient is: 40 μM, 20 μM, 10 μM, 5 μM.
[0048] Hydrogen peroxide was the...
Embodiment 3
[0053] Example 3 The protective effect of cinnamic acid compounds on hydrogen peroxide damage Escherichia coli model
[0054] 1. Experimental materials
[0055] Strains:
[0056] Escherichia coli CMCC (B) 44102 (provided by Lanzhou Food and Drug Inspection Institute, Gansu Province);
[0057] Samples for testing:
[0058] (1) Caffeic acid: prepared with cold ethanol solution, the prepared caffeic acid concentration gradient is: 20 μM, 10 μM;
[0059] (2) Ferulic acid: prepared with ethanol solution, the prepared ferulic acid concentration gradient is: 20 μM, 10 μM;
[0060] (3) p-hydroxycinnamic acid: prepared with physiological saline, the prepared p-hydroxycinnamic acid concentration gradient is: 20 μM, 10 μM;
[0061] (4) Cinnamic acid: prepared with ethanol solution, the prepared cinnamic acid concentration gradient: 20 μM, 10 μM.
[0062] figure 2 It is the structural formula of each test sample, that is, the cinnamic acid compound.
[0063] 2. Experimental method...
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