Multiplex RT-PCR detection primer for porcine delta coronavirus, porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus

A porcine epidemic diarrhea and RT-PCR technology, applied in the field of virus detection, can solve the problems of complicated operation and difficulty in effectively distinguishing virus infection, and achieve the effect of rapid differential diagnosis
CN105483291AActive Publication Date: 2016-04-13HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Publication Date
2016-04-13

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Abstract

The invention discloses a multiplex RT-PCR detection primer for a porcine delta coronavirus, a porcine epidemic diarrhea virus and a porcine transmissible gastroenteritis virus. The minimum detection capacity of the multiplex RT-PCR for the three viruses is 4.05*10<1> copies / microliter, 4.52*10<3> copies / microliter and 5.47*10<3> copies / microliter respectively. The amplification results for a porcine parvovirus (PPV) and a porcine pseudorabies virus (PRV) are both negative. The multiplex RT-PCR detection results of 57 clinical samples show that one sample is infected with the three viruses at the same time, 11 samples are infected with the PDCoV, 15 samples are infected with the PEDV, one sample is infected with the TGEV, five samples are infected with the PDCoV and the PEDV, and one sample is infected with the PDCoV and the TGEV.
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Description

technical field

[0001] The invention relates to a virus detection method, in particular to multiple RT-PCR detection primers and detection methods for porcine delta coronavirus, porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus. Background technique

[0002] Porcine Deltacoronavirus (Porcine Deltacoronavirus, PDCoV) belongs to Nidovirales (Nidovirales), Coronaviridae (Coronaviridae), Coronavirus (Coronavirus) Deltacoronavirus (Coronavirus), is a single-stranded positive-sense RNA virus with an envelope. As early as 2012, there were reports of PDCoV in the articles published by Woo et al.: They detected Delta coronavirus in mammalian and poultry disease materials collected in Guangdong and Hong Kong, among which it was detected in pig feces samples (169 samples) Nearly 10% of PDCoVs. In early 2014, a new porcine enteric coronavirus PDCoV was detected and reported for the first time in Ohio, USA. By the end of 2014, PDCoV infection cases had been...

Claims

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