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SNP marker related to rapid growth of pacific oysters and identification method as well as application thereof

A long oyster and marking technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of unsatisfactory breeding effect, large influence of human factors, low breeding efficiency, etc., and achieve rapid detection and low cost , avoid cumbersome operations, and have a strong purposeful effect

Active Publication Date: 2016-04-20
OCEAN UNIV OF CHINA
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Problems solved by technology

However, the traditional hybridization method is time-consuming and laborious, the breeding efficiency is low, and it is greatly affected by human factors, so the breeding effect is not very ideal.

Method used

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  • SNP marker related to rapid growth of pacific oysters and identification method as well as application thereof
  • SNP marker related to rapid growth of pacific oysters and identification method as well as application thereof
  • SNP marker related to rapid growth of pacific oysters and identification method as well as application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0038] 1. Screening of SNP markers related to the rapid growth of long oyster, the screening process is as follows figure 1 Shown:

[0039] 1) In July 2014, 96 individuals of the 24-month-old fast-growing oyster line were selected through continuous breeding for 5 generations, and the control group was 96 individuals from the common breeding population in Rongcheng City. A total of 192 individuals were used as the experimental population , used for preliminary screening of growth-related SNP loci; fast-growing line populations were significantly better than ordinary cultured populations in five growth indicators such as shell height and shell length (P<0.01), see Table 1.

[0040] Table 1 Comparison of growth traits between fast-growing lines and common cultured populations

[0041]

[0042] Note: The values ​​in the table are the mean ± standard error, and different letters in the same column indicate that the growth traits are significantly different between the two popu...

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Abstract

The invention discloses an SNP marker related to rapid growth of pacific oysters and an identification method as well as application thereof, and belongs to the field of molecular biological DNA marking technology and application. By applying a high-resolution melting curve analytic technique and utilizing 108 SNP loci mined from an EST common data base to perform genetic typing on pacific oyster rapid growth population after 5 generation breeding selection, 3 alleles closely related to the growth trait of pacific oysters are obtained via correlation analysis. The 3 SNP marker preponderant alleles can be applied to selection of rapid growth parent pacific oysters, and further provide a scientific auxiliary means for breeding selection of rapid growth pacific oysters. Compared with the conventional breeding method, the method has the advantages of strong purposefulness and direct action effect, besides, the operation is simple, the detection is rapid, the cost is low, and extensive popularization and application is facilitated.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP marker related to the rapid growth of oyster and its identification method and application. Background technique [0002] The long oyster (Crassostreagigas), also known as the Pacific oyster, belongs to the phyllobranch class, the pearl oyster order, and the oyster family. It is naturally distributed along the coasts of Japan, China and Korea. Long oyster has the advantages of strong environmental adaptability, fast growth, delicate meat and rich nutrition. It is the economic shellfish with the widest breeding range and the highest yield in the world. Long oyster is an important traditional cultured species in my country, but in recent years, with the improvement of the degree of intensive farming and the deterioration of the environment in the cultured sea area, the long oyster has problems such as high mortality, low meat yield, and individual miniat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李琪王九龙
Owner OCEAN UNIV OF CHINA
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