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SNP markers related to the rapid growth of long oyster and its identification method and application

A long oyster, labeling technology, applied in the directions of biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc. The effect of fast detection, low cost, avoidance of cumbersome operations, and strong purpose

Active Publication Date: 2020-05-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional hybridization method is time-consuming and laborious, the breeding efficiency is low, and it is greatly affected by human factors, so the breeding effect is not very ideal.

Method used

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  • SNP markers related to the rapid growth of long oyster and its identification method and application
  • SNP markers related to the rapid growth of long oyster and its identification method and application
  • SNP markers related to the rapid growth of long oyster and its identification method and application

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Experimental program
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Effect test

Embodiment

[0038] 1. Screening of SNP markers related to the rapid growth of Pacific oyster, the screening process is as follows figure 1 Shown:

[0039] 1) In July 2014, 96 individuals of the 24-month-old oyster fast-growing line that were continuously selected and bred for 5 generations were selected. The control group was 96 individuals from the common breeding group in Rongcheng, and a total of 192 individuals were used as the experimental group. , Used for the preliminary screening of growth-related SNP sites; the fast-growing line population is significantly better than the ordinary cultured population in terms of shell height, shell length and other 5 growth indicators (P <0.01), see Table 1.

[0040] Table 1 Comparison of growth traits between fast-growing lines and common breeding populations

[0041]

[0042] Note: The values ​​in the table are the mean ± standard error. The letters marked with different letters in the same column indicate that the growth traits are extremely differen...

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Abstract

The invention discloses an SNP marker related to rapid growth of pacific oysters and an identification method as well as application thereof, and belongs to the field of molecular biological DNA marking technology and application. By applying a high-resolution melting curve analytic technique and utilizing 108 SNP loci mined from an EST common data base to perform genetic typing on pacific oyster rapid growth population after 5 generation breeding selection, 3 alleles closely related to the growth trait of pacific oysters are obtained via correlation analysis. The 3 SNP marker preponderant alleles can be applied to selection of rapid growth parent pacific oysters, and further provide a scientific auxiliary means for breeding selection of rapid growth pacific oysters. Compared with the conventional breeding method, the method has the advantages of strong purposefulness and direct action effect, besides, the operation is simple, the detection is rapid, the cost is low, and extensive popularization and application is facilitated.

Description

Technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a SNP marker related to the rapid growth of the oyster oyster and its identification method and application. Background technique [0002] Crassostrea gigas (Crassostrea gigas), also known as Pacific oyster, belongs to the class Pelobranchia, Pearl shell order, Oysteridae, and is naturally distributed along the coast of Japan, China and Korea. Long oyster has the advantages of strong environmental adaptability, fast growth, fine meat quality and rich nutrition. It is the most widely cultivated economic shellfish with the highest yield in the world. Long oysters are traditionally important cultured species in my country. However, in recent years, with the increase in the degree of cultivation intensification and the deterioration of the environment in the cultured sea area, the long oysters have encountered problems such as high mortality, low meat yield, and indiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李琪王九龙
Owner OCEAN UNIV OF CHINA
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