68 results about "High Resolution Melt Analysis" patented technology

The invention provides a method for identifying types of herbal medicinal materials. The method comprises the following steps: obtaining a DNA sample of a herbal medicinal material to be detected; (b) performing a polymerase chain reaction between the DNA sample of the herbal medicinal material to be detected and at least one primer pair to obtain a polymerase chain reaction product; (c) performing high-resolution melting analysis on the polymerase chain reaction product to obtain a high-resolution melting analysis spectrum of the herbal medicinal material to be detected; and (d) comparing the high-resolution melting analysis spectrum of the herbal medicinal material to be detected with a high-resolution melting analysis spectrum of a known herbal medicinal material.

In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the boundDNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) onsaid bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

Compositions and process are provided for the rapid and specific detection of drug resistant forms of Mycobacterium tuberculosis based on real time PCR and high resolution melt analysis. The compositions and processes are useful for the detection of mutations within the Rifampicin Resistance Determinant Region (RRDR) of rpoB for the detection of rifampicin (RIF) and within specific regions of katG and the inhA promoter for the detection of isoniazid (INH) resistance. The invention also is capable of rapidly discriminating Mycobacterium tuberculosis complex (MTBC) strains from Nontuberculous Mycobacteria (NTM) strains.

The invention provides a human ApoE gene polymorphism detection kit based on the ARMS-PCR melting curve detection method. The human ApoE gene polymorphism detection kit comprises primers for detecting the polymorphism of an ApoE gene, and the primers include two groups of primers for detecting the c.388T>C site and the c.526C>T site respectively. The invention further provides an ARMS-PCR melting curve detection method and a PCR method of the human ApoE gene polymorphism detection kit. By the adoption of the human ApoE gene polymorphism detection kit based on the ARMS-PCR melting curve method, the ability of allele-specific primers to distinguish different alleles can be greatly improved, the difference between melting curves of two amplified allele fragments is greatly increased, the ability of SNP typing of the melting curve method is enhanced, melting curve method SNP typing can be achieved simply with an ordinary fluorescence PCR instrument instead of a high-precision high-resolution melting curve PCR instrument, the detection result is accurate, sensitivity is high, and accurate typing of a genomic DNA sample as low as 1 ng can be achieved.

The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

The invention discloses a method for detecting vibrio parahemolyticus through combination of unlabelled fluorescent PCR and HRMA, which is characterized by including the following steps: firstly, a primer pair is designed according to a TLH (thermolabile hemolysin) gene of the vibrio parahemolyticus; secondly, after DNA (Deoxyribonucleic acid) samples are extracted, unlabelled fluorescent PCR amplification is performed by utilizing the designed primer pair; and thirdly, an amplification curve of a PCR amplification product and the HRMA are analyzed by application software. The purposes of the method are to overcome defects of the prior art and provide a method, which is simple, convenient and fast to operate, accurate in detection result and low in usage cost, for detecting the vibrio parahemolyticus through the combination of the unlabelled fluorescent PCR and the HRMA.

The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for resolving a denaturation curve of a sample containing a first and second nucleic acid into a resolved denaturation curve for the first nucleic acid and a resolved denuration curve for the second nucleic acid.

The invention relates to the field of nucleic acid detection, and discloses a Zika virus detection kit based on high resolution melting analysis and a detection method thereof. The invention is characterized in that specific primers are designed according to Zika virus NS3 gene sequences, Zika virus nucleic acid is detected by using an optimized high resolution melting reaction system, so that theZika virus detection kit is simple in operations, fast in speed and free of the need of post-processing on PCR products and capable of detecting the difference of single base and truly realizing theclosed-tube operation.

The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).

The invention belongs to the field of biological medicine, and relates to a detection primer, amplification system and detection kit of a microsatellite instability site-BAT 26 site. The sequence of the primer is shown as SEQ ID NO:1 and SEQ ID NO:2. The primer is adopted to perform high-resolution melting curve method and HRM detection, and is high in sensitivity and specificity.

The invention relates to a digital PCR detection method, when the nucleic acid amplification reaction liquid to be detected is prepared, genotyping, mutation scanning, methylation research and the like can be realized by using dyes, high resolution and sensitivity are achieved, and the detection cost is reduced. Moreover, a polymerase chain reaction of a micro-droplet array can be completed on a same highly-integrated digital PCR detector, and melting curve analysis is carried out on a PCR product after PCR amplification of the micro-droplet array. Meanwhile, through the digital PCR detectionmethod, a fluorescence curve and a melting curve of the micro-droplet array can be obtained, and traceless connection between real-time monitoring of the whole PCR amplification process and melting curve analysis of the PCR product can be completely achieved. Therefore, copy number identification based on different fluorescence curves, identification of nucleic acid amplification characteristics based on different characteristic melting curves and typing, mutation scanning and the like of genes through high-resolution melting curves are realized, and digital PCR detection is completed more comprehensively, simply, conveniently and efficiently.

In one aspect, the disclosure provides methods, kits and compositions for determining the presence of a JC virus mutant in a sample.

The invention discloses a combination primer for human gall stone related gene mutation screening and application thereof, and relates to the technical field of gene detection. The combination primerfor the human gall stone related gene mutation screening is divided into a combination forward primer and a combination reverse primer; a human gall stone related gene mutation screening kit comprisesthe combination primer and LightScanner Master Mix. The invention provides a human gall stone related gene mutation screening method; the method is based on a high-resolution melting curve method; three mutation sites of common gall stone related gene can be simultaneously screened; common mutation sites such as rs1260326, rs1256049 and rs11887534 of related genes on chr2 and chr14 chromosomes are included. The characteristics of high flux, low cost and short detection time are realized.

The invention provides an in-vitro detection method for quickly screening the exon 5-8 mutation of a P53 gene. The method comprises the following steps: (1) extracting the genome DNA of a peripheral blood sample; (2) designing and synthesizing 4 pairs of specific primers for amplifying exon 5-8 of the P53 gene; (3) performing high resolution melting curve analysis and grounding PCR on the genome DNA of the sample by using the primers; and (4) obtaining the sample with a positive result in the curve analysis, meaning occurrence of P53 gene mutation. The detection method is not limited by site or type of mutated base, does not need a sequence specific probe, and can directly run high resolution melting after PCR is completed to complete analysis on the sample mutation. The method is simple, convenient and quick in operation, is low in use cost and accurate in result, can realize truly closed pipe operation. Furthermore, the method has the characteristics of high through-put, high speed, kit availability, low detection cost and the like for quick screening of population.