6 results about "High Resolution Melt Analysis" patented technology

The invention relates to the technical field of biomedical detection, and discloses a kit product for detecting SNP (Single Nucleotide Polymorphism) of human micro ribonucleic acid absorption capacity, which comprises one or more of an SNP1 site and an SNP2 site. The method has the advantages that the operation process is simple, and the capacity of the main body of the detected sample for absorbing the micro ribonucleic acid can be determined according to the condition of the SNP site only through the two steps of DNA extraction and high-resolution melting curve method PCR reaction; the detection sample is more stable and easier to store; the method can provide help for drug effect identification of drugs containing micro ribonucleic acid and determination of clinical medication of the drugs, can improve the detection efficiency, and has a wide application prospect.

The invention discloses a human MTHFR (methylenetetrahydrofolate reductase), MTRR (methylenetetrahydrofolate reductase) and SLC19A1 gene detection kit. The kit is based on a high-resolution melting curve analysis technology, and comprises four groups of primer pairs aiming at C677T and A1298C sites of MTHFR genes, A66G sites of MTRR genes and A80G sites of SLC19A1 genes. The kit can be used for rapidly, sensitively and accurately identifying the genotypes of the four key sites under the same reaction procedure by detecting the melting temperature of PCR products and the difference of curve shapes. The kit is suitable for risk assessment of cardiovascular diseases and birth defects, is more innovatively applied to donor screening and receptor assessment of coprophilous fungus transplantation, fills the blank of genetic background detection tools in the field, and has the advantages of low cost, high sensitivity, high specificity, simplicity and convenience in operation, high repeatability, quick and objective detection result and the like.

The present application belongs to the cross field of molecular diagnosis, environmental microorganism detection and nanotechnology, and particularly relates to a biological aerosol automatic detection platform, a detection method and application. The present application realizes specific capture and enrichment of target microorganisms through a silicon nanotip array, and the silicon nanotip array chip is provided with a plurality of regularly arranged silicon nanotips, and the surface of the silicon nanotip is modified with biological capture molecules. The temperature change of the nanotip is monitored in real time by using Raman spectrum, and the feedback optimization of the lysis / amplification temperature curve is realized, the double signal amplification and verification of LAMP amplification and CRISPR detection are combined, the high-resolution melting curve is analyzed through a machine learning algorithm, and the automatic identification and molecular typing of the strain are realized. The present application has the advantages of high detection sensitivity, strong specificity, simple operation, short detection time and the like. The present application is suitable for on-site rapid detection and real-time monitoring of pathogenic bacteria in various environments such as atmosphere, exhaled air and water.

The invention discloses a method for improving screening accuracy of specific primers of Shigella by using fluorescent loop-mediated isothermal amplification and a high-resolution melting curve, and belongs to the field of molecular biology. The method comprises the following steps: designing a primer group through an ipaH gene, extracting Shigella genome DNA, taking the Shigella genome DNA as a template, carrying out a fluorescent LAMP amplification reaction on the designed LAMP primer group, and carrying out HRM analysis on an amplification product; the positive amplification curve in the amplification curves is in a typical S shape, the negative control is not amplified, or the peak time of the amplification curve lags the positive peak time by more than 30 min is screened as a specific primer; in an HRM melting curve, a positive template is in a single sharp peak shape, and the positive Tm value is 90.0-91.0 DEG C; the negative control does not have a specific melting peak or has a wide and blunt non-specific peak, and the difference value between the negative control Tm and the positive Tm is greater than or equal to 2.0 DEG C. Through dual verification of fluorescent LAMP and HRM accurate analysis, the advantages and disadvantages of the primers are accurately evaluated, accurate and efficient screening of the optimal primer group is realized, and sensitive and specific primers are provided for subsequent LAMP detection tests.

Methods of determining methylation of DNA are provided that include filtering a biological sample comprising a nucleic acid with a first matrix material to purify the DNA; eluting and denaturing the DNA to produce eluted denatured DNA; heating the DNA in the presence of bisulfite ions to produce deaminated nucleic acid; optionally contacting said deaminated nucleic acid with a second matrix material; desulphonating and eluting the bound deaminated nucleic acid; and performing methylation specific PCR, nucleic acid sequencing, and / or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein multiple steps may be performed in a single reaction cartridge.