Human ApoE gene polymorphism detection kit based on ARMS-PCR melting curve method

Abstract

The invention provides a human ApoE gene polymorphism detection kit based on the ARMS-PCR melting curve detection method. The human ApoE gene polymorphism detection kit comprises primers for detecting the polymorphism of an ApoE gene, and the primers include two groups of primers for detecting the c.388T>C site and the c.526C>T site respectively. The invention further provides an ARMS-PCR melting curve detection method and a PCR method of the human ApoE gene polymorphism detection kit. By the adoption of the human ApoE gene polymorphism detection kit based on the ARMS-PCR melting curve method, the ability of allele-specific primers to distinguish different alleles can be greatly improved, the difference between melting curves of two amplified allele fragments is greatly increased, the ability of SNP typing of the melting curve method is enhanced, melting curve method SNP typing can be achieved simply with an ordinary fluorescence PCR instrument instead of a high-precision high-resolution melting curve PCR instrument, the detection result is accurate, sensitivity is high, and accurate typing of a genomic DNA sample as low as 1 ng can be achieved.

Description

technical field [0001] The invention relates to a human ApoE gene detection kit, in particular to a human ApoE gene polymorphism detection kit based on the ARMS-PCR melting curve method. Background technique [0002] Apolipoprotein E (Apolipoprotein E, ApoE) is a glycoprotein, including 299 amino acids, which plays a major role in human lipid metabolism. ApoE participates in the regulation of lipid metabolism in the body through various pathways, and is an important internal factor that affects the blood lipid level in the body. The ApoE gene is closely related to a variety of cardiovascular and cerebrovascular diseases, including hyperlipoproteinemia, atherosclerotic vascular disease, and coronary heart disease. The polymorphism of the ApoE gene is the main reason for the individual differences in the development process of these diseases and the efficacy of drug treatment. [0003] The ApoE genotype is determined by two main SNPs, namely the base at the 112th amino acid ...

AI Technical Summary

Problems solved by technology

[0013] In view of this, the present invention provides a human APOE gene polymorphism detection kit based on the ARMS-PCR melting curve method, to solve the problem of high price, complicated operation, easy pollution, and detection of detection instruments that exist in traditional detection techniques and finished product detection kits. High cost, difficult to promote clinical application and other issues

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