Combination primer for human gall stone related gene mutation screening and application thereof

A gallstone and human technology, applied in the field of combined primers for the screening of human gallstone-related gene mutations, can solve the problems of high cost and low detection efficiency, and achieve the effect of low cost, low detection cost and low cost

Inactive Publication Date: 2018-05-22
深圳市一道生物科技有限公司
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Problems solved by technology

At present, the research on the polymorphism of these gene loci is mainly based on the Sanger sequencing method,...

Method used

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  • Combination primer for human gall stone related gene mutation screening and application thereof
  • Combination primer for human gall stone related gene mutation screening and application thereof
  • Combination primer for human gall stone related gene mutation screening and application thereof

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Embodiment Construction

[0032] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0033] Primers were designed as follows:

[0034]

[0035] In order to ensure that the sample is not contaminated by food or drink, do not eat or drink within 30 minutes before sampling.

[0036] 1) Genomic DNA was extracted from oral swab samples of patients with gallstones.

[0037] 2) Different combined primers are mixed in equal proportions, and the mixed primers are used as a primer pool. Use a 96-well plate or 384-well plate, configure the reaction system, stick the plate membrane, and centrifuge briefly to collect the solution on the tube wall to the bottom of the tube; (Note: When sticking the film, use the Roche LightCycler 480Ⅱ special scraper to make the membrane stick tight PCR plate.)

[0038] The reaction system is as follows: ...

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Abstract

The invention discloses a combination primer for human gall stone related gene mutation screening and application thereof, and relates to the technical field of gene detection. The combination primerfor the human gall stone related gene mutation screening is divided into a combination forward primer and a combination reverse primer; a human gall stone related gene mutation screening kit comprisesthe combination primer and LightScanner Master Mix. The invention provides a human gall stone related gene mutation screening method; the method is based on a high-resolution melting curve method; three mutation sites of common gall stone related gene can be simultaneously screened; common mutation sites such as rs1260326, rs1256049 and rs11887534 of related genes on chr2 and chr14 chromosomes are included. The characteristics of high flux, low cost and short detection time are realized.

Description

Technical field: [0001] The invention relates to the technical field of gene detection, in particular to a combined primer for screening human gallstone-related gene mutations and an application thereof. Background technique: [0002] Gallstone is an ancient and common benign disease, which has become an important public health problem worldwide. Epidemiological surveys in western developed countries show that the incidence rate is 10%-15%, while the average incidence rate in my country at this stage is 8%. A survey shows that 25% of the incidence of gallstones is affected by genetic factors. [0003] Human gallstones may be related to the polymorphisms of rs1260326, rs1256049 and rs11887534 of related genes on chromosomes chr2 and chr14 through preliminary research. At present, the research on the polymorphism of these gene loci is mainly based on the Sanger sequencing method, which can only detect a certain region of a certain sample, and the detection efficiency is low a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q2531/113C12Q2563/107C12Q2527/107
Inventor 欧阳秦毛燕陈娟操立超郐斌李洁璇黄业胜
Owner 深圳市一道生物科技有限公司
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