Combination primer for human gall stone related gene mutation screening and application thereof
A gallstone and human technology, applied in the field of combined primers for the screening of human gallstone-related gene mutations, can solve the problems of high cost and low detection efficiency, and achieve the effect of low cost, low detection cost and low cost
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[0032] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.
[0033] Primers were designed as follows:
[0034]
[0035] In order to ensure that the sample is not contaminated by food or drink, do not eat or drink within 30 minutes before sampling.
[0036] 1) Genomic DNA was extracted from oral swab samples of patients with gallstones.
[0037] 2) Different combined primers are mixed in equal proportions, and the mixed primers are used as a primer pool. Use a 96-well plate or 384-well plate, configure the reaction system, stick the plate membrane, and centrifuge briefly to collect the solution on the tube wall to the bottom of the tube; (Note: When sticking the film, use the Roche LightCycler 480Ⅱ special scraper to make the membrane stick tight PCR plate.)
[0038] The reaction system is as follows: ...
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