Soil heavy metal detector based on urease biosensor

Abstract

The invention provides a soil heavy metal detector based on a urease biosensor. The soil heavy metal detector comprises a system control module, a potentiostat module, a signal acquisition and processing module, the urease biosensor and a serial port communication module, wherein the potentiostat module is used for providing stable constant potentials to a reference electrode and a working electrode of the urease biosensor, the signal acquisition and processing module is used for acquiring current signals generated in a substrate by the urease biosensor, performing current-to-voltage conversion, filtering, amplification and analog-digital conversion in sequence on the current signals, and then sending the signals to the system control module, the received digital signals are processed under the control of the system control module so as to obtain a soil heavy metal detection result, and the serial port communication module is used for sending the detection result to an upper computer. The soil heavy metal detector can be used for detecting heavy metal in soil quickly and accurately, and the cost and the operation complexity are lowered.

Description

technical field [0001] The invention relates to the technical field of soil heavy metal detection, in particular to a soil heavy metal detector based on a urease biosensor. Background technique [0002] At present, the quality and safety of agricultural products is one of the focuses of people's attention, and the quality of agricultural products is closely related to the quality of the soil environment. The assessment of soil environmental quality, especially the detection of heavy metals in the soil environment, is very important for the quality of agricultural products and even human health. In addition, with the acceleration of industrialization and urbanization in China, more and more industries are involved in heavy metal emissions, including mining, metal smelting, chemical industry, printing and dyeing, leather, urban garbage, waste batteries, etc., making heavy metal pollutants through different forms Into the soil environment of farmland, affecting the safety of ag...

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Problems solved by technology

[0003] At present, conventional heavy metal detection techniques mainly include X-Ray Fluorescence (XRF for short), Inductively coupled plasma mass spectrometry (ICP-MS for short), Atomic Absorption Spectroscopy (AAS for short), and Atomic Emission Spectrometry (Atomic Emission Spectrometry). , referred to as AES), atomic fluorescence spectrometry (Atomic Fluorescence Spectrometry, referred to as AFS), these methods have good selectivity and sensitivity, but there are complex sample pretreatment, expensive equipment, high operating costs and the need for professionals and in-house Due to defects such as operation in a laboratory environment, it is difficult to quickly screen a large number of soil samples on site

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