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Application of c-type natriuretic peptide in preparation of external contraceptive and test reagent of sperm function

A contraceptive and external technology, applied in the field of medicine and health, can solve health hazards and other problems, achieve the effect of less semen volume, convenient use, and simple operation method

Active Publication Date: 2018-12-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you take it blindly and in large quantities for a long time, it will cause great harm to your health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Application of C-type natriuretic peptide in inducing sperm chemotaxis

[0030] Fresh sperm were taken from the cauda epididymis of adult male mice, and incubated in capacitation solution T6 for 1.5 h for capacitation. The mouse sperm after capacitation was added to the 150 μl T6 droplet in the center of the petri dish, and the sperm density was about 5×10 6 pieces / ml. One end of the capillary (2.5 cm in length, 3 mm in diameter) was closed, and an ampulla was placed at the closed end. The ampulla was derived from the oviduct of a mouse that had just ovulated, and was used as the experimental group. In the control group, the capillary (2.5 cm in length, 3 mm in diameter) was not placed in the ampulla. The open end of the capillary is connected to a droplet of semen. Continuously observe the number of sperm in the capillary in the field of view under the 10x objective lens of the microscope. The experiment was repeated three times.

[0031] It was found th...

Embodiment 2

[0033] Example 2 Application of C-type natriuretic peptide in the detection of sperm function

[0034] Capabilize the sperm that cannot be naturally fertilized in capacitation solution T6, and then add it to 150 μl of T6 solution microdroplets, with a sperm density of 10 6 pieces / ml. Take a capillary with a length of 2.5 cm and a diameter of 3 mm, with one end closed. Take out the ampulla of the mouse that has just ovulated, put it into the closed end of the capillary, and then connect the open end of the capillary with the seminal fluid droplet. In the control group, the capillary (2.5 cm in length, 3 mm in diameter) did not contain the ampulla. Continuously observe the number of sperm in the field of view under the 10x objective lens of the microscope. At the same time, compare the number of sperm in the capillary of the treatment group and the control group. If there is no significant difference, it indicates that the reason why the semen cannot be naturally fertilized ma...

Embodiment 3

[0035] Example 3 Application of C-type natriuretic peptide in reducing the fertilization rate of mice

[0036] Adult female mice were treated with ovulation induction, injected with a certain concentration of C-type natriuretic peptide into their cervix, and then housed with male mice. The specific method is as follows: 30 adult CD-1 female mice (6-8 weeks), each weighing about 35g, were divided into five groups, six in each group, namely A, B, C, D, E groups, each group The middle female mice are numbered 1, 2, 3, 4, 5, and 6. Five groups of female mice were given different concentrations of C-type natriuretic peptide, respectively, 0, 10×10 -10 , 5×10 -10 、10 -9 、10 -8 mol / l, the injection volume is 20μl. After adult female mice were induced by gonadotropin (PMSG 5IU / mouse, induced for 48 hours, then HCG 5IU / mouse, induced for 12 hours), inject 20 microliters of C-type natriuretic peptide into the cervix of female mice with a micro-syringe, immediately Cage with adult ...

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PUM

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Abstract

The present invention provides application of C-type natriuretic peptide in the preparation of a topical contraceptive and a sperm function test reagent. It is found in studies that at the time of ovulation, C-type natriuretic peptide is expressed at the ampulla of oviduct, at a site of fertilization, and this can induce the chemotactic movement of sperms toward an ovum to accomplish the fertilization. If a certain amount of C-type natriuretic peptide is added into the genital tract of a female mammal, or the C-type natriuretic peptide is incubated with the sperms, such that the concentration gradient distribution of the C-type natriuretic peptide in the oviduct is changed, the sperms cannot chemotactically move toward the ovum along the concentration gradient, and thus the fertilization is blocked.

Description

technical field [0001] The invention relates to the field of medicine and health, in particular to the application of C-type natriuretic peptide in the preparation of external contraceptives and sperm function detection reagents. Background technique [0002] The number of male infertile populations has increased due to factors such as climate change and faster pace of life and work, and some causes of male infertility have not yet been clarified. Routine semen analysis can mainly understand the information of semen volume, sperm density and total amount of sperm in semen, sperm agglutination, sperm motility and activity rate, sperm morphology, and semen liquefaction. In fact, it is difficult to truly understand the function and fertilization potential of sperm through the above-mentioned routine analysis, especially in artificial insemination and test-tube baby, it is still not possible to decide whether artificial insemination can be performed only through the information ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17C12Q1/02A61P15/18
CPCA61K38/17A61K38/22C12Q1/02
Inventor 张美佳夏国良孔娜娜张玉李佳
Owner CHINA AGRI UNIV
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