A method for activating astrocytes using surface microstructures

A technology of glial cells and microstructure, applied in nervous system cells, animal cells, nervous system diseases, etc., to achieve the effect of avoiding glial scars and broad application prospects
CN105647868BActive Publication Date: 2020-03-31DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2020-03-31

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Abstract

The invention provides a method for activating astrocytes by using surface microstructures, which belongs to the field of cell culture technology. The specific steps are: (1) obtaining a PDMS cell culture surface with uneven microstructures; The subcultured glial cells are the activation objects. After the cells grow to the cell fusion, the cells are digested with 0.25% trypsin to make a cell suspension. (3) Add the cell suspension to the surface of the microstructure. After the cells adhere to the wall, Cultured for 1-14 days; (4) The astrocytes obtained by the above method were identified to show a series of activation performances such as EMT transformation, enhanced cell stemness, and enhanced glucose transport. The advantage of the present invention is that glial cells can be activated by using the physical microstructure on the surface of the culture, and glial scars caused by reactive hyperplasia of glial cells can be avoided. The invention has broad application prospects in the treatment of nervous system damage and degenerative diseases.
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Description

technical field

[0001] The invention relates to cell culture technology, and particularly provides a method for activating astrocytes by utilizing surface microstructures. Background technique

[0002] Astrocytes are the largest, most numerous and most widely distributed glial cells in the central nervous system, with a large number of radial processes. Astrocytes play a decisive role in neural development, synapse formation, blood-brain barrier construction, energy metabolism, etc., and are essential for maintaining brain homeostasis and normal neuron function.

[0003] Astrocytes exist in two states: resting state and activated state. When the nervous system is stimulated by various harmful factors, such as hypoxia, trauma, inflammation, etc., astrocytes transform from a resting state to an activated state and become activated astrocytes. The role of activated glial cells is a "double-edged sword". On the one hand, activated glial cells rapidly migrate and surround the ...

Claims

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