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Method for detecting heat stability of foot and mouth disease viral vaccine with hot luminescence technology and application thereof

A technology of foot-and-mouth disease virus and fluorescence technology, applied in the field of biological detection, to achieve the effects of good versatility, loss reduction, and simple operation

Inactive Publication Date: 2016-06-22
JINYUBAOLING BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even so, when faced with the detection of thermal stability of virus vaccines, there are still problems to be overcome such as mechanism and application conditions (such as the choice of fluorescent dyes) for the specific situation of viral thermal stability.

Method used

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  • Method for detecting heat stability of foot and mouth disease viral vaccine with hot luminescence technology and application thereof
  • Method for detecting heat stability of foot and mouth disease viral vaccine with hot luminescence technology and application thereof
  • Method for detecting heat stability of foot and mouth disease viral vaccine with hot luminescence technology and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1, carry out protein unfolding and nucleic acid release thermostability detection to foot-and-mouth disease virus vaccine Re-A strain with thermal fluorescence technique

[0062] Carry out protein unfolding and nucleic acid release thermostability detection to foot-and-mouth disease virus vaccine Re-A strain with thermofluorescence technique, comprise the following steps:

[0063] 1) Coordination and preparation of foot-and-mouth disease virus vaccine Re-A strain samples

[0064] FMD virus vaccine Re-A strain (in this case, from Lanzhou Veterinary Research Institute) sample coordination: the production process of FMD virus vaccine samples is completed by the production department, and the production department provides concentrated and purified FMD virus vaccine samples;

[0065] Prepare the sample of FMD virus vaccine Re-A strain: the concentration of FMD virus particles (146S) in the FMD virus vaccine sample is measured by the quality inspection department ...

Embodiment 2

[0084] Embodiment 2, using thermal fluorescence technology to detect the thermal stability of nucleic acid release to foot-and-mouth disease virus vaccine Asia-1 / JS strain and O / MYA98 strain

[0085] Carry out nucleic acid release thermostability detection to foot-and-mouth disease virus vaccine Asia-1 / JS strain and O / MYA98 strain (from Lanzhou Veterinary Research Institute) with thermal fluorescence technology, comprising the following steps:

[0086] 1) Coordination and preparation of samples of foot-and-mouth disease virus vaccine Asia-1 / JS strain and O / MYA98 strain

[0087] Coordination of samples of foot-and-mouth disease virus vaccine Asia-1 / JS strain and O / MYA98 strain: the production process of foot-and-mouth disease virus vaccine samples is completed by the production department, and the production department provides concentrated and purified foot-and-mouth disease virus vaccine samples;

[0088] Prepare the Asia-1 / JS strain and O / MYA98 strain samples of foot-and-mou...

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Abstract

The invention discloses a method for detecting heat stability of foot and mouth disease viral vaccine with a hot luminescence technology. The method includes the steps that one kind of fluorochrome namely nucleic acid dye or two kinds of fluorochrome including nucleic acid dye and protein dye are used and combined with the foot and mouth disease viral vaccine, the combination is put into a quantitative PCR amplifier to conduct gradient temperature rise, the nucleic acid release temperature Tr or protein unfolding temperature Tm of the foot and mouth disease viral vaccine are obtained through a melting curve, and the low temperature value is determined as the heat stability temperature of the foot and mouth disease viral vaccine. The method is high in universality and sensitivity, high in test speed, small in standard error, easy to operate and capable of accurately evaluating heat stability of a viral capsid, is used for detecting the heat stability of the foot and mouth disease viral vaccine and plays a role in preparing, storing and transporting the foot and mouth disease viral vaccine.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting the thermal stability of a foot-and-mouth disease virus vaccine using thermal fluorescence technology and its application in the preparation, storage and transportation of the foot-and-mouth disease virus vaccine. Background technique [0002] foot and mouth disease [0003] Foot-and-mouth disease is an acute, febrile, and contagious infectious disease common to cloven-hoofed animals caused by Foot-and-Mouth Disease Virus (FMDV) infection. The most susceptible animals are cattle, buffaloes, pigs, camels, sheep, deer, etc. Wild animals such as gazelle, musk deer, wild boar, and bison are also susceptible to the disease. The disease is most susceptible to cattle, and the infection rate of sheep is low. Foot-and-mouth disease is endemic in Asia, Africa, the Middle East, and South America, and there are sporadic cases in non-endemic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N33/68
CPCG01N33/6803C12Q1/04C12Q1/686C12Q2304/10
Inventor 王云凌张贵刚刘艳霞魏学峰刘国英范秀丽韩志玲王艳杰王秀明张宸
Owner JINYUBAOLING BIO PHARMA CO LTD
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