Rapeseed CMS Lines with Leaf Edge Fully Cracked Marker, Breeding Method and Application
A technology of cleavage and character, which is applied in the field of rapeseed sterile lines and breeding, can solve the problems of difficult identification of population purity and true and false hybrids, inconspicuous marker characters of rapeseed, and easy to be affected by the environment, etc., and achieves easy identification of restorer lines. and sterile lines, ensuring seed purity and yield, and the effect of ensuring seed purity
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Embodiment 1
[0042] The rapeseed male sterile line breeding method described in this implementation with the leaf edge full crack mark character, it specifically comprises the following steps:
[0043] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:
[0044] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;
[0045] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and the BC was obtained by backcrossing with Brassica napus (RS013-008) AACC as the male parent 1 F backcross group;
[0046] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as the fem...
Embodiment 2
[0056] The method for breeding the rapeseed male sterile line with the full-cut mark character of leaf margin described in this implementation, specifically comprises the following steps:
[0057] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:
[0058] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;
[0059] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and the BC was obtained by backcrossing with Brassica napus (RS013-008) AACC as the male parent 1 F backcross group;
[0060] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as...
Embodiment 3
[0067] The method for breeding the rapeseed male sterile line with the full-cut mark character of leaf margin described in this implementation, specifically comprises the following steps:
[0068] The process of the stable rape breeding method with leaf edge full crack marker traits is as follows: figure 2 As shown, it specifically includes the following steps:
[0069] a) F 1 Substitute ABC, with F 1 F 2 generation segregating populations, from F 2 From the segregation population of the first generation, a single plant of AABBCC with the trait of full-cut leaf margin was obtained;
[0070] b) AABBCC obtained from the F2 segregation population with the trait of full leaf margin cleavage was used as the female parent, and the BC was obtained by backcrossing with Brassica napus (RS013-008) AACC as the male parent 1 F backcross group;
[0071] c) from the BC 1 In the backcross population of F, a single plant AABBCC with the trait of fully split leaf margin was selected as...
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