Biotransformation method of agmatine sulfate

An agmatine sulfate, biotransformation technology, applied in chemical instruments and methods, organic chemistry, preparation of organic compounds, etc., can solve the problem of no agmatine sulfate biotransformation method, etc., and achieves low cost and reaction conditions. Gentle, simple process effect

Active Publication Date: 2016-06-29
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problem that there is no efficient biotransformation method for agmatine sulfate in the prior art, an embodiment of the present invention provides a biotransformation method for agmatine sulfate

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Embodiment 1

[0052] With L-arginine as substrate, arginine decarboxylase as biotransformation catalyst, and pyridoxal phosphate as coenzyme, decarboxylation reaction is carried out to generate crude agmatine, which is treated with dilute sulfuric acid Acidification to obtain the crude product of agmatine sulfate. The mass ratio of arginine decarboxylase to L-arginine is 1:40; the mass ratio of pyridoxal phosphate to L-arginine is 1:1000. The temperature of the reaction system is controlled to be 35°C; the pH of the reaction system is controlled to be 6.5.

[0053] Arginine decarboxylase is arginine decarboxylase after immobilization treatment, and the immobilization treatment of arginine decarboxylase comprises the following steps:

[0054] 1) crushing the engineering bacterial cells containing arginine decarboxylase, centrifuging the engineering bacterial cells, and collecting the arginine decarboxylase liquid;

[0055] 2) Add the arginine decarboxylase solution, additives and immobiliz...

Embodiment 2

[0066] With L-arginine as substrate, arginine decarboxylase as biotransformation catalyst, and pyridoxal phosphate as coenzyme, decarboxylation reaction is carried out to generate crude agmatine, which is treated with dilute sulfuric acid Acidification to obtain the crude product of agmatine sulfate. The mass ratio of arginine decarboxylase to L-arginine is 1:20; the mass ratio of pyridoxal phosphate to L-arginine is 1:800. The temperature of the reaction system is controlled to be 30°C; the pH of the reaction system is controlled to be 6.

[0067] Arginine decarboxylase is arginine decarboxylase after immobilization treatment, and the immobilization treatment of arginine decarboxylase comprises the following steps:

[0068] 1) crushing the engineering bacterial cells containing arginine decarboxylase, centrifuging the engineering bacterial cells, and collecting the arginine decarboxylase liquid;

[0069] 2) Add the arginine decarboxylase solution, additives and immobilized ...

Embodiment 3

[0078] With L-arginine as substrate, arginine decarboxylase as biotransformation catalyst, and pyridoxal phosphate as coenzyme, decarboxylation reaction is carried out to generate crude agmatine, which is treated with dilute sulfuric acid Acidification to obtain the crude product of agmatine sulfate. The mass ratio of arginine decarboxylase to L-arginine is 1:30; the mass ratio of pyridoxal phosphate to L-arginine is 1:500. The temperature of the reaction system is controlled to be 42°C; the pH of the reaction system is controlled to be 7.

[0079] Arginine decarboxylase is arginine decarboxylase after immobilization treatment, and the immobilization treatment of arginine decarboxylase comprises the following steps:

[0080] 1) crushing the engineering bacterial cells containing arginine decarboxylase, centrifuging the engineering bacterial cells, and collecting the arginine decarboxylase liquid;

[0081] 2) Add the arginine decarboxylase solution, additives and immobilized ...

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Abstract

The invention discloses a biotransformation method of agmatine sulfate and belongs to the field of pharmaceutical intermediates. The method comprises steps as follows: L-arginine taken as a substrate and arginine decarboxylase taken as a biotransformation catalyst are subjected to a decarboxylic reaction to produce a coarse agmatine product, the coarse agmatine product is acidized with dilute sulfuric acid, and a coarse agmatine sulfate product is obtained. The method adopts the simple technological process, has no special requirement for equipment and is applicable to industrial production; compared with a simple chemical method, the method has the advantages that the reaction condition is mild, the operation is simple and convenient, the cost is lower, the pollution is low, and an enzyme catalytic reaction which is difficult to conduct in the chemical reaction can be finished.

Description

technical field [0001] The invention relates to the field of preparation of pharmaceutical intermediates, in particular to a biotransformation method of agmatine sulfate. Background technique [0002] Agmatine is the product of L-arginine decarboxylase (L-ADC) catalyzing the decarboxylation of arginine. It is a neurotransmitter that is distributed in most organs and tissues of mammals. Its specificity Exist in certain cells, such as adrenal chromaffin cells, glial cells and globular cells of the carotid body. Research data show that agmatine has biological activities such as lowering blood sugar, lowering blood pressure, diuresis, anti-inflammation, anti-depression, and inhibiting cell proliferation, especially for N-methyl-D-aspartate (NMDA) receptors. The antagonism is strong and long-lasting, and it has a withdrawal effect on morphine dependence in animals. It is a kind of detoxification drug with great development value. And agmatine works by stimulating the release of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12N11/14C07C277/08C07C279/12
CPCC07C277/08C12N11/14C12P13/001C07C279/12
Inventor 陈建华
Owner JIANGHAN UNIVERSITY
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