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Preparation method and application of spica prunellae polysaccharide extracts

A technology for polysaccharide extraction and Prunella vulgaris, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients to achieve the effects of inhibiting and inducing the proliferation of vascular smooth muscle cells, enriching extraction methods, and saving extraction time.

Active Publication Date: 2016-07-13
湖南丛桂草堂医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on its enzyme-assisted ultrasonic hot water extraction of polysaccharides from Prunella vulgaris

Method used

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  • Preparation method and application of spica prunellae polysaccharide extracts

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Experimental program
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Effect test

Embodiment 1

[0039] The preparation method step of the present embodiment Prunella vulgaris polysaccharide extract is as follows:

[0040] (1) Pretreatment of medicinal materials:

[0041] Grind the dried fruit ears of Labiatae plant Prunella vulgaris (Prunellavulgaris L.) to 60 mesh, add ethanol solution with a volume concentration of 95% at a liquid-to-solid ratio of 20mL / g, reflux and extract at 60°C for 5h, filter the residue, and collect the filter residue Place it in the air to dry naturally, and set it aside to obtain the Prunella vulgaris powder from which lipids, pigments and small molecular sugars have been removed.

[0042] (2) Extraction:

[0043] Take 150 g of Prunella vulgaris powder obtained in the step (1), add 5% cellulase solution at a ratio of 45 mL / g to liquid in an ultrasonic reactor, soak at 60° C. for 150 min, and then 65 Extract at ℃ for 2 hours, the extracted solution is centrifuged at 3000 rpm, and the supernatant A is collected after 10 minutes;

[0044] (3) C...

Embodiment 2

[0056] The preparation method step of the present embodiment Prunella vulgaris polysaccharide extract is as follows:

[0057] (1) Pretreatment of medicinal materials:

[0058] Grind the dried fruit ears of Prunella vulgaris L., a Labiatae plant, to 60 meshes, add an ethanol solution with a volume concentration of 95% at a liquid-to-solid ratio of 15mL / g, reflux and extract at 75°C for 4.5h, filter the residue, and collect The filter residue is placed in the air to dry naturally, and it is set aside to obtain the Prunella vulgaris powder from which lipids, pigments and small molecular sugars have been removed.

[0059] (2) Extraction:

[0060] Take 150 g of Prunella vulgaris powder obtained in the step (1), add 4.5% cellulase solution at a ratio of 50 mL / g to liquid in an ultrasonic reactor, soak for 140 min at 65° C. ℃ for 2.5h extraction, the extracted solution was centrifuged at 3000 rpm, and the supernatant A was collected after 12min;

[0061] (3) Concentration:

[006...

Embodiment 3

[0073] The preparation method step of the present embodiment Prunella vulgaris polysaccharide extract is as follows:

[0074] (1) Pretreatment of medicinal materials:

[0075] Grind the dried fruit ears of Labiatae plant Prunella vulgaris L. to 80 mesh, add 90% ethanol solution at a ratio of 10mL / g to liquid, reflux and extract at 55°C for 5h, filter the residue, and collect the filter residue Place it in the air to dry naturally, and set it aside to obtain the Prunella vulgaris powder from which lipids, pigments and small molecular sugars have been removed.

[0076] (2) Extraction:

[0077] Take 150 g of Prunella vulgaris powder obtained in the step (1), add 5.5% cellulase solution at a ratio of 30 mL / g to liquid in an ultrasonic reactor, soak and enzymolyze it for 160 min at 55° C. Extract at ℃ for 3 hours, centrifuge the extracted solution at 2500 rpm, and collect the supernatant A after 10 minutes;

[0078] (3) Concentration:

[0079] Supernatant A was concentrated by ...

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Abstract

The invention discloses a preparation method of spica prunellae polysaccharide extracts.The method comprises the following steps that spica prunellae is smashed and added into an ethanol water solution on the basis that the ratio of solid to liquid is 10-20 mL / g, reflux extraction is performed, and filtering and drying are performed to obtain spica prunellae powder; the spica prunellae powder is added into a cellulase water solution on the basis that the ratio of solid to liquid is 30-50 mL / g, soaking and enzymolysis are performed, ultrasonic extraction is performed, and a supernate is collected; concentration is performed; absolute ethyl alcohol with the volume being 3-4 times that of the obtained concentrated product is added into the obtained concentrated product, alcohol precipitation is performed, and precipitations are collected; the obtained precipitations are dissolved into a small amount of water and added into a n-butyl alcohol-chloroform solution with the volume being one quarter that of the precipitations on the basis of the volume ratio being 4:1, stirring, standing and configuring are performed, and a supernate is collected to obtain a deproteinization polysaccharide solution; secondary alcohol precipitation is performed, and precipitations are collected to obtain deproteinization polysaccharide; the obtained deproteinization is freezen and dried to obtain dried spica prunellae polysaccharide extract powder.The method is high in spica prunellae polysaccharide extraction rate, simple in technology, green, free of toxicity, environmentally friendly and suitable for industrial popularization.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method and application of a polysaccharide extract from Prunella vulgaris. Background technique [0002] Prunella vulgaris is the dried ear of Prunella vulgaris L., a plant of the Labiatae family. In my country, Prunella vulgaris is concentrated in the Huaihe River Basin and the middle and lower reaches of the Yangtze River, and the wild resources in Jiangsu, Anhui, Zhejiang and Henan are the most abundant; South Korea, Japan, Ukraine and Kashmir are also distributed. The main components of Prunella vulgaris are triterpenes and their saponins, sterols, flavonoids, coumarins, polysaccharides, etc. Prunella vulgaris has a long history of medicinal use. Its main effects include clearing the liver and purging fire, improving eyesight, dissipating stagnation and reducing swelling; Carbuncle, breast addiction, distending pain in the breast ("Pharmac...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P9/14
CPCA61K31/715A61K36/536C08B37/0003
Inventor 林丽美夏伯候廖端芳周亚敏
Owner 湖南丛桂草堂医药有限公司
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