Establishment method for model for promoting tongue cancer development and lymphatic metastasis of mammals

A technology of mammalian and lymphatic transfer, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, inorganic active ingredients, etc.

Inactive Publication Date: 2016-08-17
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drinking 4-NQO alone, increasing the experimental period (more than 30 weeks), although lymphatic metastasis will al...

Method used

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  • Establishment method for model for promoting tongue cancer development and lymphatic metastasis of mammals
  • Establishment method for model for promoting tongue cancer development and lymphatic metastasis of mammals
  • Establishment method for model for promoting tongue cancer development and lymphatic metastasis of mammals

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Experimental program
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Embodiment Construction

[0033] The construction method of promoting mouse tongue cancer development and lymphatic metastasis model of the present invention:

[0034] (1) Selection of experimental animals and grouping: take 6-week-old female C57BL / 6J mice as experimental mice, and randomly divide them into experimental group 1, experimental group 2, experimental group 3 and control group; the conditions of experimental mice are: female C57BL / 6J mice, clean grade, with a body weight of 19.15±0.45g, were fed at a room temperature of 18-25°C, a humidity of 30%-50%, standard feed, and 24 hours of alternating light and dark conditions;

[0035] (2) The preparation of the medicine used in the experiment: the powdered reagent 4-NQO was made into 100 mg / L solution A with sterile water, and placed in a water bottle for the mice to drink freely; the NaNO 2 Make 500mg / L solution B with sterile water, put it in a water bottle and let the mice drink freely;

[0036] (3) Dosing cycle: first feed the mice with solu...

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Abstract

The invention relates to the field of animal models and particularly discloses an establishment method for a model for promoting tongue cancer development and lymphatic metastasis of mammals. According to the establishment method for the model for promoting tongue cancer development and lymphatic metastasis of the mammals, the mammals are fed with a 4-NQO solution and a NaNO2 solution in sequence for a period of time; the mammals are subjected to normal observation every week and killed randomly for HE staining observation and immunohistochemistry CK8 staining observation; when white patches are generated on the tongue faces of the mammals, invasive carcinoma is found through HE staining observation, and lymphatic metastasis is generated through CK8 staining. The establishment method for the animal model for promoting tongue cancer development and lymphatic metastasis with sodium nitrite is established, the progress of the actually-generated tongue cancer is simulated, and a novel and powerful tool is provided for research on the influences of sodium nitrite on the tongue cancer, treatment research on the tongue cancer and the like.

Description

technical field [0001] The invention relates to the field of animal models, and specifically discloses a method for promoting the development of mammalian tongue cancer and constructing a lymphatic metastasis model. Background technique [0002] 1.1 Tongue squamous cell carcinoma [0003] Tongue cancer, most of which are tongue squamous cell carcinoma (TSCC) of epithelial origin, can be divided into tongue body cancer (front 2 / 3 of tongue) and tongue base cancer (back 1 / 3 of tongue) according to anatomical classification. 3) Two types, among which the incidence of tongue cancer is higher. The tongue is one of the important tissues and organs of the oral and maxillofacial region. Its anatomical structure and physiological functions are extremely complex. Tumor cell invasion, frequent tongue movement and rich lymphatic drainage, so metastasis is very easy to occur. [0004] The occurrence of tongue cancer is related to the repeated injury, congestion and hyperplasia of tong...

Claims

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Application Information

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IPC IPC(8): A61K33/00
CPCA61K33/00
Inventor 张雁余姝毅郑骏恒肖嘉盈
Owner SUN YAT SEN UNIV
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