Significance analysis method

An analysis method and significant technology, applied in the field of significance analysis, can solve problems such as high error, and achieve the effect of reducing differential screening error and high accuracy

Inactive Publication Date: 2016-08-24
TARIM UNIV
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Problems solved by technology

When studying the difference between the two groups of samples, we take the differential gene as the research object for further research. Since th...

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Embodiment Construction

[0027] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0028] Significance Analysis of Pathway——Pathway-Analysis

[0029] Pathway-Analysis is a significant pathway analysis of all Pathways involved in genes, mainly using fisher's exact test and χ 2 To test, use the following four tables:

[0030]

[0031] no f : Indicates the number of differential genes in the pathway.

[0032] N f : Indicates the number of differential genes.

[0033] n: Indicates the total number of genes contained in the pathway.

[0034] N: Indicates the total number of genes detected on the chip.

[0035] p 1 : Indicates the probability that the differential gene falls in the pathway.

[0036] p 2 : Indicates the probability that the differential allogene does not fall in the pathway.

[0037] Hypothesis: H 0 :p 1 =p 2 ,

[0038] h 1 :p 1 ≠p 2 ,

[0039] Under this assumption, Fisher's exact test a...

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Abstract

The invention discloses a significance analysis method. A p value and a p (k) value are obtained through utilizing fisher accurate checking and x2 checking respectively; an FDR (False Positive Rate) of a pathway is determined through a plurality of times of comparing and checking. Under the condition of a small sample, differential gene screening is carried out through commonly utilizing t checking or F checking of a random variance model; with regard to two types of differential genes, the difference is identified by adopting the t checking. In order to reduce differential screening errors caused by the small sample, two groups are compared through utilizing T checking revised by the random variance model to calculate a significance level and the false positive rate between the genes, so that the differential genes are obtained; the method has the characteristic of high accuracy and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of genes, and relates to a significance analysis method, in particular to a pathway significance analysis method. Background technique [0002] The full name of KEGG (Kyoto Encyclopedia of Genes and Genomes) is the Kyoto Encyclopedia of Genes and Genomes. It is a database that systematically analyzes the relationship between genes (and their encoded products), gene functions, and genome information. It helps researchers to combine genes and expression information. Research the network as a whole. Genomic information is stored in the GENES database, including complete and partially sequenced genome sequences; more advanced functional information is stored in the PATHWAY database, including graphical cell biochemical processes such as metabolism, membrane transport, signal transmission, cell cycle, and homologous conservation sub-channels and other information. The integrated metabolic pathway (pathway) quer...

Claims

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Application Information

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IPC IPC(8): G06F19/24G06F19/16G06F19/12
CPCG16B5/00G16B15/00G16B40/00
Inventor 贺建忠刘坤陈宏伟王永常卫华白万胜郭心怀
Owner TARIM UNIV
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