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Culture liquid for animal cells, and culture vessel

A technology of animal cells and culture containers, applied in the direction of animal cells, vertebrate cells, culture process, etc., can solve problems such as uncertainty and ambiguity, and achieve the effect of high cell proliferation efficiency

Inactive Publication Date: 2016-10-26
TOYO SEIKAN GRP HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, if oxidative phosphorylation that can fully utilize the performance of a highly gas-permeable culture container is performed, it is not certain whether the growth efficiency of cells can be improved as expected.
In addition, it is not clear how to increase the ratio of oxidative phosphorylation in cellular respiration

Method used

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  • Culture liquid for animal cells, and culture vessel
  • Culture liquid for animal cells, and culture vessel
  • Culture liquid for animal cells, and culture vessel

Examples

Experimental program
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Effect test

Embodiment

[0075] Hereinafter, for the experiments conducted in order to confirm the effects of the animal cell culture medium and the culture container according to the embodiment of the present invention, refer to Figure 4 ~ Figure 12 Be explained.

[0076] [Experiment 1]

[0077] Cell culture using a highly gas-permeable culture container filled with the culture solution for animal cells of this embodiment (Example 1), and culture using a culture medium for animal cells filled with this embodiment were carried out under the following conditions, respectively. Cell culture in a normal gas-permeable culture vessel (Example 2), cell culture in a highly gas-permeable culture vessel filled with a conventional animal cell culture medium (Comparative Example 1), and Cell culture in a conventional gas-permeable culture container filled with a conventional culture solution for animal cells (Comparative Example 2).

[0078] (1) Culture medium for animal cells

[0079] First, as a convention...

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Abstract

Provided is a culture liquid for animal cells, which enables the performance of a highly gas-permeable culture vessel to be exhibited satisfactorily and also enables high cell growth efficiency to be achieved. A culture liquid for animal cells, which can be used for improving the metabolism activity of a TCA cycle (a tricarboxylic acid cycle) in the respiration of animal cells, said culture liquid containing, as an active ingredient, an amino acid that can be metabolized into succinyl CoA, which is an element constituting the TCA cycle, at a concentration of 2.3 to 6.0 mmol / L inclusive.

Description

technical field [0001] The present invention relates to a cell culture technique, in particular to a culture solution for animal cells and a culture container for improving the proliferation efficiency of animal cells. Background technique [0002] In recent years, in fields such as the production of pharmaceuticals, gene therapy, regenerative medicine, and immune cell therapy, it is required to efficiently culture large quantities of cells, tissues, and microorganisms in an artificial environment. [0003] Under such circumstances, cells and a culture solution are enclosed in a culture vessel made of a gas-permeable membrane, and a large number of cells are cultured in a closed system. [0004] In order to obtain excellent cell proliferation efficiency in such cell culture, the present applicant has developed a multilayer film with high gas permeability, and has completed a highly gas permeable culture container using the multilayer film (refer to patent Literature 1). It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12M1/00
CPCC12N5/0018C12N2500/32C12N5/0645C12N5/0634C12M23/24C12N1/00C12M1/00
Inventor 太田恭平田中乡史
Owner TOYO SEIKAN GRP HLDG LTD
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